Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing
Polydopamine can form biocompatible particles that convert light into heat. Recently, a protocol has been optimized to synthesize polydopamine/protein hybrid nanoparticles that retain the biological function of proteins, and combine it with the stimuli-induced heat generation of polydopamine. We hav...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/8/12/1065 |
| _version_ | 1819156725147631616 |
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| author | Daniel Hauser Manuela Estermann Ana Milosevic Lukas Steinmetz Dimitri Vanhecke Dedy Septiadi Barbara Drasler Alke Petri-Fink Vincent Ball Barbara Rothen-Rutishauser |
| author_facet | Daniel Hauser Manuela Estermann Ana Milosevic Lukas Steinmetz Dimitri Vanhecke Dedy Septiadi Barbara Drasler Alke Petri-Fink Vincent Ball Barbara Rothen-Rutishauser |
| author_sort | Daniel Hauser |
| collection | DOAJ |
| description | Polydopamine can form biocompatible particles that convert light into heat. Recently, a protocol has been optimized to synthesize polydopamine/protein hybrid nanoparticles that retain the biological function of proteins, and combine it with the stimuli-induced heat generation of polydopamine. We have utilized this novel system to form polydopamine particles, containing transferrin (PDA/Tf). Mouse melanoma cells, which strongly express the transferrin receptor, were exposed to PDA/Tf nanoparticles (NPs) and, subsequently, were irradiated with a UV laser. The cell death rate was monitored in real-time. When irradiated, the melanoma cells exposed to PDA/Tf NPs underwent apoptosis, faster than the control cells, pointing towards the ability of PDA/Tf to mediate UV-light-induced cell death. The system was also validated in an organotypic, 3D-printed tumor spheroid model, comprising mouse melanoma cells, and the exposure and subsequent irradiation with UV-light, yielded similar results to the 2D cell culture. The process of apoptosis was found to be targeted and mediated by the lysosomal membrane permeabilization. Therefore, the herein presented polydopamine/protein NPs constitute a versatile and stable system for cancer cell-targeting and photothermal apoptosis induction. |
| first_indexed | 2024-12-22T15:57:26Z |
| format | Article |
| id | doaj.art-39c2880a080549e99b27c58f7990e17e |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-22T15:57:26Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-39c2880a080549e99b27c58f7990e17e2022-12-21T18:20:44ZengMDPI AGNanomaterials2079-49912018-12-01812106510.3390/nano8121065nano8121065Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-KillingDaniel Hauser0Manuela Estermann1Ana Milosevic2Lukas Steinmetz3Dimitri Vanhecke4Dedy Septiadi5Barbara Drasler6Alke Petri-Fink7Vincent Ball8Barbara Rothen-Rutishauser9Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, SwitzerlandAdolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, SwitzerlandAdolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, SwitzerlandAdolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, SwitzerlandAdolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, SwitzerlandAdolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, SwitzerlandAdolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, SwitzerlandAdolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, SwitzerlandUniversité de Strasbourg, Faculté de Chirurgie Dentaire, 8 Rue Sainte Elisabeth, 67000 Strasbourg, FranceAdolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, SwitzerlandPolydopamine can form biocompatible particles that convert light into heat. Recently, a protocol has been optimized to synthesize polydopamine/protein hybrid nanoparticles that retain the biological function of proteins, and combine it with the stimuli-induced heat generation of polydopamine. We have utilized this novel system to form polydopamine particles, containing transferrin (PDA/Tf). Mouse melanoma cells, which strongly express the transferrin receptor, were exposed to PDA/Tf nanoparticles (NPs) and, subsequently, were irradiated with a UV laser. The cell death rate was monitored in real-time. When irradiated, the melanoma cells exposed to PDA/Tf NPs underwent apoptosis, faster than the control cells, pointing towards the ability of PDA/Tf to mediate UV-light-induced cell death. The system was also validated in an organotypic, 3D-printed tumor spheroid model, comprising mouse melanoma cells, and the exposure and subsequent irradiation with UV-light, yielded similar results to the 2D cell culture. The process of apoptosis was found to be targeted and mediated by the lysosomal membrane permeabilization. Therefore, the herein presented polydopamine/protein NPs constitute a versatile and stable system for cancer cell-targeting and photothermal apoptosis induction.https://www.mdpi.com/2079-4991/8/12/1065cell targetinglysosomal membrane permeabilizationpolydopamine/transferrin nanoparticleslive cell imagingtargeted apoptosis in vitro3D cell printingspheroids |
| spellingShingle | Daniel Hauser Manuela Estermann Ana Milosevic Lukas Steinmetz Dimitri Vanhecke Dedy Septiadi Barbara Drasler Alke Petri-Fink Vincent Ball Barbara Rothen-Rutishauser Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing Nanomaterials cell targeting lysosomal membrane permeabilization polydopamine/transferrin nanoparticles live cell imaging targeted apoptosis in vitro 3D cell printing spheroids |
| title | Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing |
| title_full | Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing |
| title_fullStr | Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing |
| title_full_unstemmed | Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing |
| title_short | Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing |
| title_sort | polydopamine transferrin hybrid nanoparticles for targeted cell killing |
| topic | cell targeting lysosomal membrane permeabilization polydopamine/transferrin nanoparticles live cell imaging targeted apoptosis in vitro 3D cell printing spheroids |
| url | https://www.mdpi.com/2079-4991/8/12/1065 |
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