A new inhibitor of apoptosis from vaccinia virus and eukaryotes.
A new apoptosis inhibitor is described from vaccinia virus, camelpox virus, and eukaryotic cells. The inhibitor is a hydrophobic, multiple transmembrane protein that is resident in the Golgi and is named GAAP (Golgi anti-apoptotic protein). Stable expression of both viral GAAP (v-GAAP) and human GAA...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2007-02-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.0030017 |
| _version_ | 1819007573236383744 |
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| author | Caroline Gubser Daniele Bergamaschi Michael Hollinshead Xin Lu Frank J M van Kuppeveld Geoffrey L Smith |
| author_facet | Caroline Gubser Daniele Bergamaschi Michael Hollinshead Xin Lu Frank J M van Kuppeveld Geoffrey L Smith |
| author_sort | Caroline Gubser |
| collection | DOAJ |
| description | A new apoptosis inhibitor is described from vaccinia virus, camelpox virus, and eukaryotic cells. The inhibitor is a hydrophobic, multiple transmembrane protein that is resident in the Golgi and is named GAAP (Golgi anti-apoptotic protein). Stable expression of both viral GAAP (v-GAAP) and human GAAP (h-GAAP), which is expressed in all human tissues tested, inhibited apoptosis induced by intrinsic and extrinsic apoptotic stimuli. Conversely, knockout of h-GAAP by siRNA induced cell death by apoptosis. v-GAAP and h-GAAP display overlapping functions as shown by the ability of v-GAAP to complement for the loss of h-GAAP. Lastly, deletion of the v-GAAP gene from vaccinia virus did not affect virus replication in cell culture, but affected virus virulence in a murine infection model. This study identifies a new regulator of cell death that is highly conserved in evolution from plants to insects, amphibians, mammals, and poxviruses. |
| first_indexed | 2024-12-21T00:26:43Z |
| format | Article |
| id | doaj.art-39c2bb64e76d4093bc90f7110fc7b7b5 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-21T00:26:43Z |
| publishDate | 2007-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-39c2bb64e76d4093bc90f7110fc7b7b52022-12-21T19:21:58ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742007-02-0132e1710.1371/journal.ppat.0030017A new inhibitor of apoptosis from vaccinia virus and eukaryotes.Caroline GubserDaniele BergamaschiMichael HollinsheadXin LuFrank J M van KuppeveldGeoffrey L SmithA new apoptosis inhibitor is described from vaccinia virus, camelpox virus, and eukaryotic cells. The inhibitor is a hydrophobic, multiple transmembrane protein that is resident in the Golgi and is named GAAP (Golgi anti-apoptotic protein). Stable expression of both viral GAAP (v-GAAP) and human GAAP (h-GAAP), which is expressed in all human tissues tested, inhibited apoptosis induced by intrinsic and extrinsic apoptotic stimuli. Conversely, knockout of h-GAAP by siRNA induced cell death by apoptosis. v-GAAP and h-GAAP display overlapping functions as shown by the ability of v-GAAP to complement for the loss of h-GAAP. Lastly, deletion of the v-GAAP gene from vaccinia virus did not affect virus replication in cell culture, but affected virus virulence in a murine infection model. This study identifies a new regulator of cell death that is highly conserved in evolution from plants to insects, amphibians, mammals, and poxviruses.https://doi.org/10.1371/journal.ppat.0030017 |
| spellingShingle | Caroline Gubser Daniele Bergamaschi Michael Hollinshead Xin Lu Frank J M van Kuppeveld Geoffrey L Smith A new inhibitor of apoptosis from vaccinia virus and eukaryotes. PLoS Pathogens |
| title | A new inhibitor of apoptosis from vaccinia virus and eukaryotes. |
| title_full | A new inhibitor of apoptosis from vaccinia virus and eukaryotes. |
| title_fullStr | A new inhibitor of apoptosis from vaccinia virus and eukaryotes. |
| title_full_unstemmed | A new inhibitor of apoptosis from vaccinia virus and eukaryotes. |
| title_short | A new inhibitor of apoptosis from vaccinia virus and eukaryotes. |
| title_sort | new inhibitor of apoptosis from vaccinia virus and eukaryotes |
| url | https://doi.org/10.1371/journal.ppat.0030017 |
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