Development of a highly sensitive lateral flow strip device for nucleic acid detection using molecular beacons
Extensive research is focused on the development of highly sensitive, rapid on-site diagnostic devices. The lateral flow strip (LFS) is a paper-based point-of-care diagnostic device, which is highly promising because of its ease of use and low cost. Despite these advantages, LFS device is still less...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Sensors |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsens.2022.1012775/full |
| _version_ | 1797989884792668160 |
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| author | Youngkwang Moon Hyeokgyun Moon Junhyuck Chang Harold D. Kim Jung Heon Lee Jung Heon Lee Jinkee Lee Jinkee Lee |
| author_facet | Youngkwang Moon Hyeokgyun Moon Junhyuck Chang Harold D. Kim Jung Heon Lee Jung Heon Lee Jinkee Lee Jinkee Lee |
| author_sort | Youngkwang Moon |
| collection | DOAJ |
| description | Extensive research is focused on the development of highly sensitive, rapid on-site diagnostic devices. The lateral flow strip (LFS) is a paper-based point-of-care diagnostic device, which is highly promising because of its ease of use and low cost. Despite these advantages, LFS device is still less popular than other methods such as enzyme-linked immunosorbent assay (ELISA) or real-time polymerase chain reaction (qPCR) due to its low sensitivity. Here, we have developed a fluorescence-based lateral flow strip (f-LFS) device for DNA detection using a molecular beacon (MB), a short hairpin-forming DNA strand tagged with a fluorophore-quencher pair. Each paper and membrane component of f-LFS device was carefully selected based on their physicochemical properties including porosity, surface functionality, and autofluorescence. The limit of detection (LOD) of this device was substantially improved to 2.1 fg/mL by adding MgCl2 to the reaction buffer and narrowing the test membrane dimension. Also, a portable fluorescence detection system for f-LFS was developed using a multi-pixel photon counter (MPPC), a sensitive detector detecting the signal on site. We anticipate that this highly sensitive paper-based diagnostic device can be utilized for on-site diagnosis of various diseases. |
| first_indexed | 2024-04-11T08:27:45Z |
| format | Article |
| id | doaj.art-39c5c3ff1ca7458f915d4dd06aa5c279 |
| institution | Directory Open Access Journal |
| issn | 2673-5067 |
| language | English |
| last_indexed | 2024-04-11T08:27:45Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sensors |
| spelling | doaj.art-39c5c3ff1ca7458f915d4dd06aa5c2792022-12-22T04:34:41ZengFrontiers Media S.A.Frontiers in Sensors2673-50672022-10-01310.3389/fsens.2022.10127751012775Development of a highly sensitive lateral flow strip device for nucleic acid detection using molecular beaconsYoungkwang Moon0Hyeokgyun Moon1Junhyuck Chang2Harold D. Kim3Jung Heon Lee4Jung Heon Lee5Jinkee Lee6Jinkee Lee7School of Mechanical Engineering, Sungkyunkwan University, Suwon, South KoreaSchool of Mechanical Engineering, Sungkyunkwan University, Suwon, South KoreaSchool of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, South KoreaSchool of Physics, Georgia Institute of Technology, Atlanta, GA, United StatesSchool of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, South KoreaInstitute of Quantum Biophysics, Sungkyunkwan University, Suwon, South KoreaSchool of Mechanical Engineering, Sungkyunkwan University, Suwon, South KoreaInstitute of Quantum Biophysics, Sungkyunkwan University, Suwon, South KoreaExtensive research is focused on the development of highly sensitive, rapid on-site diagnostic devices. The lateral flow strip (LFS) is a paper-based point-of-care diagnostic device, which is highly promising because of its ease of use and low cost. Despite these advantages, LFS device is still less popular than other methods such as enzyme-linked immunosorbent assay (ELISA) or real-time polymerase chain reaction (qPCR) due to its low sensitivity. Here, we have developed a fluorescence-based lateral flow strip (f-LFS) device for DNA detection using a molecular beacon (MB), a short hairpin-forming DNA strand tagged with a fluorophore-quencher pair. Each paper and membrane component of f-LFS device was carefully selected based on their physicochemical properties including porosity, surface functionality, and autofluorescence. The limit of detection (LOD) of this device was substantially improved to 2.1 fg/mL by adding MgCl2 to the reaction buffer and narrowing the test membrane dimension. Also, a portable fluorescence detection system for f-LFS was developed using a multi-pixel photon counter (MPPC), a sensitive detector detecting the signal on site. We anticipate that this highly sensitive paper-based diagnostic device can be utilized for on-site diagnosis of various diseases.https://www.frontiersin.org/articles/10.3389/fsens.2022.1012775/fullpaper-based diagnostic devicemolecular beacon (MB)nucleic acid detectionpoint-of-care (POC)lateral flow strip (LFS) |
| spellingShingle | Youngkwang Moon Hyeokgyun Moon Junhyuck Chang Harold D. Kim Jung Heon Lee Jung Heon Lee Jinkee Lee Jinkee Lee Development of a highly sensitive lateral flow strip device for nucleic acid detection using molecular beacons Frontiers in Sensors paper-based diagnostic device molecular beacon (MB) nucleic acid detection point-of-care (POC) lateral flow strip (LFS) |
| title | Development of a highly sensitive lateral flow strip device for nucleic acid detection using molecular beacons |
| title_full | Development of a highly sensitive lateral flow strip device for nucleic acid detection using molecular beacons |
| title_fullStr | Development of a highly sensitive lateral flow strip device for nucleic acid detection using molecular beacons |
| title_full_unstemmed | Development of a highly sensitive lateral flow strip device for nucleic acid detection using molecular beacons |
| title_short | Development of a highly sensitive lateral flow strip device for nucleic acid detection using molecular beacons |
| title_sort | development of a highly sensitive lateral flow strip device for nucleic acid detection using molecular beacons |
| topic | paper-based diagnostic device molecular beacon (MB) nucleic acid detection point-of-care (POC) lateral flow strip (LFS) |
| url | https://www.frontiersin.org/articles/10.3389/fsens.2022.1012775/full |
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