Association of CYP1A1 M2 (A2455G) polymorphism with susceptibility to breast cancer in Mazandaran Province, Northern Iran: A case–control study
Background: Breast cancer is one of the most frequent women malignancies in the world. The cytochrome P450 1A1 (CYP1A1) is a key enzyme in xenobiotics metabolism. Moreover, CYP1A1 plays a critical role in the etiology of breast cancer by involving in 2-hydroxylation of estrogen. Therefore, single-nu...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | International Journal of Preventive Medicine |
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| Online Access: | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2019;volume=10;issue=1;spage=92;epage=92;aulast=Khalili-Tanha |
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| author | Ghazaleh Khalili-Tanha Ali Barzegar Novin Nikbakhsh Zarbakht Ansari-Pirsaraei |
| author_facet | Ghazaleh Khalili-Tanha Ali Barzegar Novin Nikbakhsh Zarbakht Ansari-Pirsaraei |
| author_sort | Ghazaleh Khalili-Tanha |
| collection | DOAJ |
| description | Background: Breast cancer is one of the most frequent women malignancies in the world. The cytochrome P450 1A1 (CYP1A1) is a key enzyme in xenobiotics metabolism. Moreover, CYP1A1 plays a critical role in the etiology of breast cancer by involving in 2-hydroxylation of estrogen. Therefore, single-nucleotide polymorphisms (SNPs) of its coding gene have been verified to be important in cancer susceptibility. The aim of the study was to evaluate the association of CYP1A1 M2 (A2455G) includes rs1048943 of this SNP polymorphism with the risk of breast cancer in Mazandaran province. Methods: Ninety-six breast cancer patients with known clinicopathological characters and 110 healthy women as control were genotyped for CYP1A1 M2 polymorphisms by the restriction fragment length polymorphism technique. Results: The analysis of CYP1A1 gene (polymorphism M2) showed that the frequency of homozygous wild genotypes (AA), heterozygous (AG), and mutant genotype (GG) in the patient group, respectively, 78%, 22%, and 0%, and also the frequency of genotypes AA, AG, and GG in healthy included 82%, 16%, and 2%, respectively. Statistical analysis by Logistic regression model at P < 0.05 showed no significant correlation between polymorphisms in CYP 1A1M2 and breast cancer risk (odds ratio = 0.84, confidence interval = 0.33–2.17). Conclusions: The results indicated that the M2 allelic genotypes were significantly associated neither with breast cancer risk nor with clinicopathological characteristics in Mazandaran province. |
| first_indexed | 2024-04-13T21:46:09Z |
| format | Article |
| id | doaj.art-39c7721ea21a4ea99c9a2c3531d3c7cb |
| institution | Directory Open Access Journal |
| issn | 2008-7802 2008-8213 |
| language | English |
| last_indexed | 2024-04-13T21:46:09Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Preventive Medicine |
| spelling | doaj.art-39c7721ea21a4ea99c9a2c3531d3c7cb2022-12-22T02:28:34ZengWolters Kluwer Medknow PublicationsInternational Journal of Preventive Medicine2008-78022008-82132019-01-01101929210.4103/ijpvm.IJPVM_57_18Association of CYP1A1 M2 (A2455G) polymorphism with susceptibility to breast cancer in Mazandaran Province, Northern Iran: A case–control studyGhazaleh Khalili-TanhaAli BarzegarNovin NikbakhshZarbakht Ansari-PirsaraeiBackground: Breast cancer is one of the most frequent women malignancies in the world. The cytochrome P450 1A1 (CYP1A1) is a key enzyme in xenobiotics metabolism. Moreover, CYP1A1 plays a critical role in the etiology of breast cancer by involving in 2-hydroxylation of estrogen. Therefore, single-nucleotide polymorphisms (SNPs) of its coding gene have been verified to be important in cancer susceptibility. The aim of the study was to evaluate the association of CYP1A1 M2 (A2455G) includes rs1048943 of this SNP polymorphism with the risk of breast cancer in Mazandaran province. Methods: Ninety-six breast cancer patients with known clinicopathological characters and 110 healthy women as control were genotyped for CYP1A1 M2 polymorphisms by the restriction fragment length polymorphism technique. Results: The analysis of CYP1A1 gene (polymorphism M2) showed that the frequency of homozygous wild genotypes (AA), heterozygous (AG), and mutant genotype (GG) in the patient group, respectively, 78%, 22%, and 0%, and also the frequency of genotypes AA, AG, and GG in healthy included 82%, 16%, and 2%, respectively. Statistical analysis by Logistic regression model at P < 0.05 showed no significant correlation between polymorphisms in CYP 1A1M2 and breast cancer risk (odds ratio = 0.84, confidence interval = 0.33–2.17). Conclusions: The results indicated that the M2 allelic genotypes were significantly associated neither with breast cancer risk nor with clinicopathological characteristics in Mazandaran province.http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2019;volume=10;issue=1;spage=92;epage=92;aulast=Khalili-Tanhabreast neoplasmscytochrome p-450iranpolymorphismrestriction fragment length |
| spellingShingle | Ghazaleh Khalili-Tanha Ali Barzegar Novin Nikbakhsh Zarbakht Ansari-Pirsaraei Association of CYP1A1 M2 (A2455G) polymorphism with susceptibility to breast cancer in Mazandaran Province, Northern Iran: A case–control study International Journal of Preventive Medicine breast neoplasms cytochrome p-450 iran polymorphism restriction fragment length |
| title | Association of CYP1A1 M2 (A2455G) polymorphism with susceptibility to breast cancer in Mazandaran Province, Northern Iran: A case–control study |
| title_full | Association of CYP1A1 M2 (A2455G) polymorphism with susceptibility to breast cancer in Mazandaran Province, Northern Iran: A case–control study |
| title_fullStr | Association of CYP1A1 M2 (A2455G) polymorphism with susceptibility to breast cancer in Mazandaran Province, Northern Iran: A case–control study |
| title_full_unstemmed | Association of CYP1A1 M2 (A2455G) polymorphism with susceptibility to breast cancer in Mazandaran Province, Northern Iran: A case–control study |
| title_short | Association of CYP1A1 M2 (A2455G) polymorphism with susceptibility to breast cancer in Mazandaran Province, Northern Iran: A case–control study |
| title_sort | association of cyp1a1 m2 a2455g polymorphism with susceptibility to breast cancer in mazandaran province northern iran a case control study |
| topic | breast neoplasms cytochrome p-450 iran polymorphism restriction fragment length |
| url | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2019;volume=10;issue=1;spage=92;epage=92;aulast=Khalili-Tanha |
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