A Na<sup>+</sup>/H<sup>+</sup> Antiporter Gene from <i>Rosa multiflora</i> (<i>RmNHX2</i>) Functions in Salt Tolerance via Modulating ROS Levels and Ion Homeostasis
High salinity restricts plant growth and geographic distribution. Plant intracellular Na<sup>+</sup>/H<sup>+</sup> (NHX) antiporters have critical roles in plant development and stress response. However, the molecular functions of <i>RmNHXs</i> in <i>Rosa mu...
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MDPI AG
2023-02-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/3/290 |
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| author | Haiyan Luo Yuxiao Shen Linmei Chen Yongyi Cui Ping Luo |
| author_facet | Haiyan Luo Yuxiao Shen Linmei Chen Yongyi Cui Ping Luo |
| author_sort | Haiyan Luo |
| collection | DOAJ |
| description | High salinity restricts plant growth and geographic distribution. Plant intracellular Na<sup>+</sup>/H<sup>+</sup> (NHX) antiporters have critical roles in plant development and stress response. However, the molecular functions of <i>RmNHXs</i> in <i>Rosa multiflora</i> remain unclear. In this study, we identified 11 putative <i>RmNHXs</i> in <i>R. multiflora</i> according to the genome-wide analysis. The <i>RmNHX</i>s were classified into three classes. Most of the <i>RmNHX</i>s were responsive to salt stress, with the greatest upregulation being observed in <i>RmNHX2</i>. <i>RmNHX2</i> was localized at the tonoplast. <i>RmNHX2</i> overexpression resulted in the enhanced salt tolerance in tobacco, whereas virus-induced gene silencing (VIGS) of <i>RmNHX2</i> in <i>R. multiflora</i> elevated salt susceptibility. Under salt treatment, the transgenic tobaccos achieved less reactive oxygen species (ROS) accumulation and higher activities of antioxidant enzymes, which complied with the upregulated expressions of antioxidant genes. Moreover, <i>RmNHX2-</i>overexpression lines had a lower level of Na<sup>+</sup>, a higher level of K<sup>+</sup>, and a lower Na/K ratio. In contrast to the mentioned, VIGS of <i>RmNHX2</i> in <i>R. multiflora</i> exhibited the opposite phenotype, accompanied by a compromised salt tolerance. Regarded together, these results demonstrate that <i>RmNHX2</i> enhances plant salt tolerance by maintaining proper ion homeostasis, as well as by accelerating ROS scavenging. |
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| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T06:27:52Z |
| publishDate | 2023-02-01 |
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| series | Horticulturae |
| spelling | doaj.art-39c789237e474c4685b02e026cff8fc42023-11-17T11:23:37ZengMDPI AGHorticulturae2311-75242023-02-019329010.3390/horticulturae9030290A Na<sup>+</sup>/H<sup>+</sup> Antiporter Gene from <i>Rosa multiflora</i> (<i>RmNHX2</i>) Functions in Salt Tolerance via Modulating ROS Levels and Ion HomeostasisHaiyan Luo0Yuxiao Shen1Linmei Chen2Yongyi Cui3Ping Luo4College of Horticulture Science, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Zhejiang Moutainous, Zhejiang A & F University, Hangzhou 311300, ChinaCollege of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Horticulture Science, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Zhejiang Moutainous, Zhejiang A & F University, Hangzhou 311300, ChinaCollege of Horticulture Science, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Zhejiang Moutainous, Zhejiang A & F University, Hangzhou 311300, ChinaCollege of Horticulture Science, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Zhejiang Moutainous, Zhejiang A & F University, Hangzhou 311300, ChinaHigh salinity restricts plant growth and geographic distribution. Plant intracellular Na<sup>+</sup>/H<sup>+</sup> (NHX) antiporters have critical roles in plant development and stress response. However, the molecular functions of <i>RmNHXs</i> in <i>Rosa multiflora</i> remain unclear. In this study, we identified 11 putative <i>RmNHXs</i> in <i>R. multiflora</i> according to the genome-wide analysis. The <i>RmNHX</i>s were classified into three classes. Most of the <i>RmNHX</i>s were responsive to salt stress, with the greatest upregulation being observed in <i>RmNHX2</i>. <i>RmNHX2</i> was localized at the tonoplast. <i>RmNHX2</i> overexpression resulted in the enhanced salt tolerance in tobacco, whereas virus-induced gene silencing (VIGS) of <i>RmNHX2</i> in <i>R. multiflora</i> elevated salt susceptibility. Under salt treatment, the transgenic tobaccos achieved less reactive oxygen species (ROS) accumulation and higher activities of antioxidant enzymes, which complied with the upregulated expressions of antioxidant genes. Moreover, <i>RmNHX2-</i>overexpression lines had a lower level of Na<sup>+</sup>, a higher level of K<sup>+</sup>, and a lower Na/K ratio. In contrast to the mentioned, VIGS of <i>RmNHX2</i> in <i>R. multiflora</i> exhibited the opposite phenotype, accompanied by a compromised salt tolerance. Regarded together, these results demonstrate that <i>RmNHX2</i> enhances plant salt tolerance by maintaining proper ion homeostasis, as well as by accelerating ROS scavenging.https://www.mdpi.com/2311-7524/9/3/290<i>Rosa multiflora</i><i>RmNHX2</i>salt toleranceion homeostasisROS scavenging |
| spellingShingle | Haiyan Luo Yuxiao Shen Linmei Chen Yongyi Cui Ping Luo A Na<sup>+</sup>/H<sup>+</sup> Antiporter Gene from <i>Rosa multiflora</i> (<i>RmNHX2</i>) Functions in Salt Tolerance via Modulating ROS Levels and Ion Homeostasis Horticulturae <i>Rosa multiflora</i> <i>RmNHX2</i> salt tolerance ion homeostasis ROS scavenging |
| title | A Na<sup>+</sup>/H<sup>+</sup> Antiporter Gene from <i>Rosa multiflora</i> (<i>RmNHX2</i>) Functions in Salt Tolerance via Modulating ROS Levels and Ion Homeostasis |
| title_full | A Na<sup>+</sup>/H<sup>+</sup> Antiporter Gene from <i>Rosa multiflora</i> (<i>RmNHX2</i>) Functions in Salt Tolerance via Modulating ROS Levels and Ion Homeostasis |
| title_fullStr | A Na<sup>+</sup>/H<sup>+</sup> Antiporter Gene from <i>Rosa multiflora</i> (<i>RmNHX2</i>) Functions in Salt Tolerance via Modulating ROS Levels and Ion Homeostasis |
| title_full_unstemmed | A Na<sup>+</sup>/H<sup>+</sup> Antiporter Gene from <i>Rosa multiflora</i> (<i>RmNHX2</i>) Functions in Salt Tolerance via Modulating ROS Levels and Ion Homeostasis |
| title_short | A Na<sup>+</sup>/H<sup>+</sup> Antiporter Gene from <i>Rosa multiflora</i> (<i>RmNHX2</i>) Functions in Salt Tolerance via Modulating ROS Levels and Ion Homeostasis |
| title_sort | na sup sup h sup sup antiporter gene from i rosa multiflora i i rmnhx2 i functions in salt tolerance via modulating ros levels and ion homeostasis |
| topic | <i>Rosa multiflora</i> <i>RmNHX2</i> salt tolerance ion homeostasis ROS scavenging |
| url | https://www.mdpi.com/2311-7524/9/3/290 |
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