Microfluidic electrospray photo-crosslinkable κ-Carrageenan microparticles for wound healing
When organs, especially external organs are injured, pathogens and excessive water loss can threaten the wound healing process and cause local irreversible tissue damage. Here, novel natural κ-Carrageenan microparticles (MPs) able to deliver bioactive proteins and small molecules to the wound surfac...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-01-01
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| Series: | Engineered Regeneration |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666138121000177 |
| _version_ | 1819282452350238720 |
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| author | Zhiqiang Luo Junyi Che Lingyu Sun Lei Yang Yan Zu Huan Wang Yuanjin Zhao |
| author_facet | Zhiqiang Luo Junyi Che Lingyu Sun Lei Yang Yan Zu Huan Wang Yuanjin Zhao |
| author_sort | Zhiqiang Luo |
| collection | DOAJ |
| description | When organs, especially external organs are injured, pathogens and excessive water loss can threaten the wound healing process and cause local irreversible tissue damage. Here, novel natural κ-Carrageenan microparticles (MPs) able to deliver bioactive proteins and small molecules to the wound surface for promoted regeneration were developed using microfluidic electrospray. The side chains of κ-Carrageenan were modified with methacrylate pendant groups to make the monomer photonic cross-linkable. By changing the concentration of methacrylated-κ-Carrageenan, different porosities of MPs with controllable drug loading were achieved. The size of the MPs can also be tailored by adjusting the voltage of the applied electric field. Vascular endothelial growth factor (VEGF) and antimicrobial peptides Eumenitin were co-loaded into the microparticles (VEGF-Eumenitin-MPs) and released sustainedly in phosphate-buffered saline. The VEGF-Eumenitin-MPs had high biocompatibility and intensive antibacterial activities for bacteria. Furthermore, the antibacterial and angiogenetic properties of the VEGF-Eumenitin-MPs were demonstrated in a infectious wound model and the chronic wound healing process was significantly enhanced in vivo. Therefore, the developed porous MPs with sustained drug release properties have the potential to serve as a platform to deliver bioactive proteins and small molecules to the wound surface for promoted regeneration. |
| first_indexed | 2024-12-24T01:15:49Z |
| format | Article |
| id | doaj.art-39c86fc10d4c4724aa75f90ed84d02b9 |
| institution | Directory Open Access Journal |
| issn | 2666-1381 |
| language | English |
| last_indexed | 2024-12-24T01:15:49Z |
| publishDate | 2021-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Engineered Regeneration |
| spelling | doaj.art-39c86fc10d4c4724aa75f90ed84d02b92022-12-21T17:22:46ZengKeAi Communications Co., Ltd.Engineered Regeneration2666-13812021-01-012257262Microfluidic electrospray photo-crosslinkable κ-Carrageenan microparticles for wound healingZhiqiang Luo0Junyi Che1Lingyu Sun2Lei Yang3Yan Zu4Huan Wang5Yuanjin Zhao6Department of Rheumatology and Immunology, Institute of Translational Medicine, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaDepartment of Rheumatology and Immunology, Institute of Translational Medicine, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Rheumatology and Immunology, Institute of Translational Medicine, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China; Oujiang Laboratory, Zhejiang Lab for Regenerative Medicine, Vision and Brain Health, Wenzhou, Zhejiang 325001, China; Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang 325001, ChinaOujiang Laboratory, Zhejiang Lab for Regenerative Medicine, Vision and Brain Health, Wenzhou, Zhejiang 325001, China; Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang 325001, China; Corresponding author at: Oujiang Laboratory, Zhejiang Lab for Regenerative Medicine, Vision and Brain Health, Wenzhou, Zhejiang 325001, China.The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen 518033, China; Corresponding author at: The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen 518033, ChinaDepartment of Rheumatology and Immunology, Institute of Translational Medicine, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China; Corresponding author at: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China..When organs, especially external organs are injured, pathogens and excessive water loss can threaten the wound healing process and cause local irreversible tissue damage. Here, novel natural κ-Carrageenan microparticles (MPs) able to deliver bioactive proteins and small molecules to the wound surface for promoted regeneration were developed using microfluidic electrospray. The side chains of κ-Carrageenan were modified with methacrylate pendant groups to make the monomer photonic cross-linkable. By changing the concentration of methacrylated-κ-Carrageenan, different porosities of MPs with controllable drug loading were achieved. The size of the MPs can also be tailored by adjusting the voltage of the applied electric field. Vascular endothelial growth factor (VEGF) and antimicrobial peptides Eumenitin were co-loaded into the microparticles (VEGF-Eumenitin-MPs) and released sustainedly in phosphate-buffered saline. The VEGF-Eumenitin-MPs had high biocompatibility and intensive antibacterial activities for bacteria. Furthermore, the antibacterial and angiogenetic properties of the VEGF-Eumenitin-MPs were demonstrated in a infectious wound model and the chronic wound healing process was significantly enhanced in vivo. Therefore, the developed porous MPs with sustained drug release properties have the potential to serve as a platform to deliver bioactive proteins and small molecules to the wound surface for promoted regeneration.http://www.sciencedirect.com/science/article/pii/S2666138121000177MicroparticleHydrogelMicrofluidicsWound healingVEGFAntimicrobial peptide |
| spellingShingle | Zhiqiang Luo Junyi Che Lingyu Sun Lei Yang Yan Zu Huan Wang Yuanjin Zhao Microfluidic electrospray photo-crosslinkable κ-Carrageenan microparticles for wound healing Engineered Regeneration Microparticle Hydrogel Microfluidics Wound healing VEGF Antimicrobial peptide |
| title | Microfluidic electrospray photo-crosslinkable κ-Carrageenan microparticles for wound healing |
| title_full | Microfluidic electrospray photo-crosslinkable κ-Carrageenan microparticles for wound healing |
| title_fullStr | Microfluidic electrospray photo-crosslinkable κ-Carrageenan microparticles for wound healing |
| title_full_unstemmed | Microfluidic electrospray photo-crosslinkable κ-Carrageenan microparticles for wound healing |
| title_short | Microfluidic electrospray photo-crosslinkable κ-Carrageenan microparticles for wound healing |
| title_sort | microfluidic electrospray photo crosslinkable κ carrageenan microparticles for wound healing |
| topic | Microparticle Hydrogel Microfluidics Wound healing VEGF Antimicrobial peptide |
| url | http://www.sciencedirect.com/science/article/pii/S2666138121000177 |
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