Exploring the potential anti-inflammatory effect of biosynthesized gold nanoparticles using Isodon excisus leaf tissue in human keratinocytes
The skin plays a crucial role in maintaining hydration, preventing dehydration, and protecting against harmful microorganisms. Skin injury can lead to inflammation and fluid loss, which are detrimental to human health. Nanotechnology has been proposed as a potential solution to prevent skin damage b...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223005750 |
| _version_ | 1797690913644871680 |
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| author | Sanjeevram Dhandapani Rongbo Wang Ki cheol Hwang Hoon Kim Yeon-Ju Kim |
| author_facet | Sanjeevram Dhandapani Rongbo Wang Ki cheol Hwang Hoon Kim Yeon-Ju Kim |
| author_sort | Sanjeevram Dhandapani |
| collection | DOAJ |
| description | The skin plays a crucial role in maintaining hydration, preventing dehydration, and protecting against harmful microorganisms. Skin injury can lead to inflammation and fluid loss, which are detrimental to human health. Nanotechnology has been proposed as a potential solution to prevent skin damage because it can more effectively penetrate the skin barrier and deliver targeted treatments directly to the cells that require them. In this study, we biosynthesized gold nanoparticles (AuNPs) using Isodon excisus (Maxim.) Kudo (IE) leaf tissue. The formation of IE gold nanoparticles (IE-AuNPs) was confirmed using various techniques. Furthermore, the cytotoxicity of IE-AuNPs on human keratinocytes (HaCaT cells) was evaluated, and it was found that the IE-AuNPs had no cytotoxic effect on HaCaT cells (50 µg/mL). The ability of IE-AuNPs to penetrate HaCaT cells and reduce skin inflammation was investigated using dark-field microscopy (DFM) and enzyme-linked immunosorbent assay (ELISA). Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to assess inflammatory biomarkers such as IL-6, IL-8, CCL17/TARC, CCL27/CTACK, and CCL5/RANTES. Additionally, western blotting demonstrated that IE-AuNPs exhibited anti-inflammatory effects on the skin by activating autophagy biomarkers and inhibiting the PI3K/AKT signaling pathway at the upstream level. Overall, our findings suggest that IE-AuNPs enhance the anti-inflammatory effect on skin cells and can be used in the development of nano-based treatments for skin-related diseases. |
| first_indexed | 2024-03-12T02:05:56Z |
| format | Article |
| id | doaj.art-39cda5ff2fea4e4894bc2fda46f9e6a0 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-12T02:05:56Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-39cda5ff2fea4e4894bc2fda46f9e6a02023-09-07T04:43:27ZengElsevierArabian Journal of Chemistry1878-53522023-10-011610105113Exploring the potential anti-inflammatory effect of biosynthesized gold nanoparticles using Isodon excisus leaf tissue in human keratinocytesSanjeevram Dhandapani0Rongbo Wang1Ki cheol Hwang2Hoon Kim3Yeon-Ju Kim4Graduate School of Biotechnology, and College of Life Science, Kyung Hee University, Yongin-si, 17104, Gyeonggi-do, Republic of KoreaGraduate School of Biotechnology, and College of Life Science, Kyung Hee University, Yongin-si, 17104, Gyeonggi-do, Republic of KoreaRafarophe Co, Venture Research Center, Cheongju, Republic of KoreaDepartment of Food and Nutrition, Chung Ang University, Seodong-daero 4726, Daedeok-myeon, Anseong 17546, Republic of Korea; Corresponding authors at: Graduate School of Biotechnology, and College of Life Science, Kyung Hee University, Yongin-si, 17104, Gyeonggi-do, Republic of Korea.Graduate School of Biotechnology, and College of Life Science, Kyung Hee University, Yongin-si, 17104, Gyeonggi-do, Republic of Korea; Corresponding authors at: Graduate School of Biotechnology, and College of Life Science, Kyung Hee University, Yongin-si, 17104, Gyeonggi-do, Republic of Korea.The skin plays a crucial role in maintaining hydration, preventing dehydration, and protecting against harmful microorganisms. Skin injury can lead to inflammation and fluid loss, which are detrimental to human health. Nanotechnology has been proposed as a potential solution to prevent skin damage because it can more effectively penetrate the skin barrier and deliver targeted treatments directly to the cells that require them. In this study, we biosynthesized gold nanoparticles (AuNPs) using Isodon excisus (Maxim.) Kudo (IE) leaf tissue. The formation of IE gold nanoparticles (IE-AuNPs) was confirmed using various techniques. Furthermore, the cytotoxicity of IE-AuNPs on human keratinocytes (HaCaT cells) was evaluated, and it was found that the IE-AuNPs had no cytotoxic effect on HaCaT cells (50 µg/mL). The ability of IE-AuNPs to penetrate HaCaT cells and reduce skin inflammation was investigated using dark-field microscopy (DFM) and enzyme-linked immunosorbent assay (ELISA). Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to assess inflammatory biomarkers such as IL-6, IL-8, CCL17/TARC, CCL27/CTACK, and CCL5/RANTES. Additionally, western blotting demonstrated that IE-AuNPs exhibited anti-inflammatory effects on the skin by activating autophagy biomarkers and inhibiting the PI3K/AKT signaling pathway at the upstream level. Overall, our findings suggest that IE-AuNPs enhance the anti-inflammatory effect on skin cells and can be used in the development of nano-based treatments for skin-related diseases.http://www.sciencedirect.com/science/article/pii/S1878535223005750Isodon excisusGold nano-based treatmentsSkin inflammationAutophagyPI3K/AKT signaling pathway |
| spellingShingle | Sanjeevram Dhandapani Rongbo Wang Ki cheol Hwang Hoon Kim Yeon-Ju Kim Exploring the potential anti-inflammatory effect of biosynthesized gold nanoparticles using Isodon excisus leaf tissue in human keratinocytes Arabian Journal of Chemistry Isodon excisus Gold nano-based treatments Skin inflammation Autophagy PI3K/AKT signaling pathway |
| title | Exploring the potential anti-inflammatory effect of biosynthesized gold nanoparticles using Isodon excisus leaf tissue in human keratinocytes |
| title_full | Exploring the potential anti-inflammatory effect of biosynthesized gold nanoparticles using Isodon excisus leaf tissue in human keratinocytes |
| title_fullStr | Exploring the potential anti-inflammatory effect of biosynthesized gold nanoparticles using Isodon excisus leaf tissue in human keratinocytes |
| title_full_unstemmed | Exploring the potential anti-inflammatory effect of biosynthesized gold nanoparticles using Isodon excisus leaf tissue in human keratinocytes |
| title_short | Exploring the potential anti-inflammatory effect of biosynthesized gold nanoparticles using Isodon excisus leaf tissue in human keratinocytes |
| title_sort | exploring the potential anti inflammatory effect of biosynthesized gold nanoparticles using isodon excisus leaf tissue in human keratinocytes |
| topic | Isodon excisus Gold nano-based treatments Skin inflammation Autophagy PI3K/AKT signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223005750 |
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