Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers
Engineering of the skeletal muscles has attracted attention for the restoration of damaged muscles from myopathy, injury, and extraction of malignant tumors. Reconstructing a three-dimensional muscle using living cells could be a promising approach. However, the regenerated tissue exhibits a weak co...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-06-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/10/6/399 |
| _version_ | 1818658227883081728 |
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| author | Shinako Bansai Takashi Morikura Hiroaki Onoe Shogo Miyata |
| author_facet | Shinako Bansai Takashi Morikura Hiroaki Onoe Shogo Miyata |
| author_sort | Shinako Bansai |
| collection | DOAJ |
| description | Engineering of the skeletal muscles has attracted attention for the restoration of damaged muscles from myopathy, injury, and extraction of malignant tumors. Reconstructing a three-dimensional muscle using living cells could be a promising approach. However, the regenerated tissue exhibits a weak construction force due to the insufficient tissue maturation. The purpose of this study is to establish the reconstruction system for the skeletal muscle. We used a cell-laden core-shell hydrogel microfiber as a three-dimensional culture to control the cellular orientation. Moreover, to mature the muscle tissue in the microfiber, we also developed a custom-made culture device for imposing cyclic stretch stimulation using a motorized stage and the fiber-grab system. As a result, the directions of the myotubes were oriented and the mature myotubes could be formed by cyclic stretch stimulation. |
| first_indexed | 2024-12-17T03:54:02Z |
| format | Article |
| id | doaj.art-39d0359df72e4a19a495809f39e9799e |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-17T03:54:02Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-39d0359df72e4a19a495809f39e9799e2022-12-21T22:04:40ZengMDPI AGMicromachines2072-666X2019-06-0110639910.3390/mi10060399mi10060399Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel FibersShinako Bansai0Takashi Morikura1Hiroaki Onoe2Shogo Miyata3Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, JapanGraduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, JapanDepartment of Mechanical Engineering, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, JapanDepartment of Mechanical Engineering, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, JapanEngineering of the skeletal muscles has attracted attention for the restoration of damaged muscles from myopathy, injury, and extraction of malignant tumors. Reconstructing a three-dimensional muscle using living cells could be a promising approach. However, the regenerated tissue exhibits a weak construction force due to the insufficient tissue maturation. The purpose of this study is to establish the reconstruction system for the skeletal muscle. We used a cell-laden core-shell hydrogel microfiber as a three-dimensional culture to control the cellular orientation. Moreover, to mature the muscle tissue in the microfiber, we also developed a custom-made culture device for imposing cyclic stretch stimulation using a motorized stage and the fiber-grab system. As a result, the directions of the myotubes were oriented and the mature myotubes could be formed by cyclic stretch stimulation.https://www.mdpi.com/2072-666X/10/6/399myoblastskeletal musclecore-shell hydrogel fibercyclic stretchengineered muscle |
| spellingShingle | Shinako Bansai Takashi Morikura Hiroaki Onoe Shogo Miyata Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers Micromachines myoblast skeletal muscle core-shell hydrogel fiber cyclic stretch engineered muscle |
| title | Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers |
| title_full | Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers |
| title_fullStr | Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers |
| title_full_unstemmed | Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers |
| title_short | Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers |
| title_sort | effect of cyclic stretch on tissue maturation in myoblast laden hydrogel fibers |
| topic | myoblast skeletal muscle core-shell hydrogel fiber cyclic stretch engineered muscle |
| url | https://www.mdpi.com/2072-666X/10/6/399 |
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