Genetic determinants of mycotoxin synthesis in genus Fusarium
The fungi of the genus Fusarium occur worldwide mainly on cereal grains. They are considered to be not only plant-pathogenic, but also the producers of secondary metabolites - mycotoxins. In our study, we focused on the analysis by Q-PCR of two genes expression - Tri4, Tri10 - involved in the produc...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2011-12-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201110-0015_genetic-determinants-of-mycotoxin-synthesis-in-genus-fusarium.php |
| _version_ | 1797899733702803456 |
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| author | Hana Havránková Jarmila Pazlarová Jaroslava Ovesná |
| author_facet | Hana Havránková Jarmila Pazlarová Jaroslava Ovesná |
| author_sort | Hana Havránková |
| collection | DOAJ |
| description | The fungi of the genus Fusarium occur worldwide mainly on cereal grains. They are considered to be not only plant-pathogenic, but also the producers of secondary metabolites - mycotoxins. In our study, we focused on the analysis by Q-PCR of two genes expression - Tri4, Tri10 - involved in the production of Fusarium mycotoxin deoxynivalenol. Reference genes β-tub and UBC were found to be suitable for the expression level normalisation using the program geNorm. The level of expression of the target genes was analysed on four isolates of Fusarium graminearum grown in vitro. In all four isolates, Tri10 gene expression level was lower than that of Tri4 gene expression. |
| first_indexed | 2024-04-10T08:34:39Z |
| format | Article |
| id | doaj.art-39d59db1fd8b435e849cc4093132e3fb |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:34:39Z |
| publishDate | 2011-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-39d59db1fd8b435e849cc4093132e3fb2023-02-23T03:27:40ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172011-12-0129Special IssueS86S9310.17221/314/2011-CJFScjf-201110-0015Genetic determinants of mycotoxin synthesis in genus FusariumHana Havránková0Jarmila Pazlarová1Jaroslava Ovesná2Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech RepublicDepartment of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech RepublicThe fungi of the genus Fusarium occur worldwide mainly on cereal grains. They are considered to be not only plant-pathogenic, but also the producers of secondary metabolites - mycotoxins. In our study, we focused on the analysis by Q-PCR of two genes expression - Tri4, Tri10 - involved in the production of Fusarium mycotoxin deoxynivalenol. Reference genes β-tub and UBC were found to be suitable for the expression level normalisation using the program geNorm. The level of expression of the target genes was analysed on four isolates of Fusarium graminearum grown in vitro. In all four isolates, Tri10 gene expression level was lower than that of Tri4 gene expression.https://cjfs.agriculturejournals.cz/artkey/cjf-201110-0015_genetic-determinants-of-mycotoxin-synthesis-in-genus-fusarium.phpfusariumtrichothecene biosynthesistri4tri10real-time pcrgenorm |
| spellingShingle | Hana Havránková Jarmila Pazlarová Jaroslava Ovesná Genetic determinants of mycotoxin synthesis in genus Fusarium Czech Journal of Food Sciences fusarium trichothecene biosynthesis tri4 tri10 real-time pcr genorm |
| title | Genetic determinants of mycotoxin synthesis in genus Fusarium |
| title_full | Genetic determinants of mycotoxin synthesis in genus Fusarium |
| title_fullStr | Genetic determinants of mycotoxin synthesis in genus Fusarium |
| title_full_unstemmed | Genetic determinants of mycotoxin synthesis in genus Fusarium |
| title_short | Genetic determinants of mycotoxin synthesis in genus Fusarium |
| title_sort | genetic determinants of mycotoxin synthesis in genus fusarium |
| topic | fusarium trichothecene biosynthesis tri4 tri10 real-time pcr genorm |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201110-0015_genetic-determinants-of-mycotoxin-synthesis-in-genus-fusarium.php |
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