Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content>
ABSTRACT The vast majority of annotated transcripts in bacteria are mRNAs. Here we identify ~1,000 antisense transcripts in the model bacterium Escherichia coli. We propose that these transcripts are generated by promiscuous transcription initiation within genes and that many of them regulate expres...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2010-05-01
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| Series: | mBio |
| Online Access: | https://journals.asm.org/doi/10.1128/mBio.00024-10 |
| _version_ | 1818740156365012992 |
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| author | James E. Dornenburg Anne M. DeVita Michael J. Palumbo Joseph T. Wade |
| author_facet | James E. Dornenburg Anne M. DeVita Michael J. Palumbo Joseph T. Wade |
| author_sort | James E. Dornenburg |
| collection | DOAJ |
| description | ABSTRACT The vast majority of annotated transcripts in bacteria are mRNAs. Here we identify ~1,000 antisense transcripts in the model bacterium Escherichia coli. We propose that these transcripts are generated by promiscuous transcription initiation within genes and that many of them regulate expression of the overlapping gene. IMPORTANCE The vast majority of known genes in bacteria are protein coding, and there are very few known antisense transcripts within these genes, i.e., RNAs that are encoded opposite the gene. Here we demonstrate the existence of ~1,000 antisense RNAs in the model bacterium Escherichia coli. Given the high potential for these RNAs to base pair with mRNA of the overlapping gene and the likelihood of clashes between transcription complexes of antisense and sense transcripts, we propose that antisense RNAs represent an important but overlooked class of regulatory molecule. |
| first_indexed | 2024-12-18T01:36:15Z |
| format | Article |
| id | doaj.art-39d6736279744c988881d8bc48371103 |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-12-18T01:36:15Z |
| publishDate | 2010-05-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-39d6736279744c988881d8bc483711032022-12-21T21:25:26ZengAmerican Society for MicrobiologymBio2150-75112010-05-011110.1128/mBio.00024-10Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content>James E. Dornenburg0Anne M. DeVita1Michael J. Palumbo2Joseph T. Wade3Wadsworth Center, New York State Department of Health, Albany, New York, USAWadsworth Center, New York State Department of Health, Albany, New York, USAWadsworth Center, New York State Department of Health, Albany, New York, USAWadsworth Center, New York State Department of Health, Albany, New York, USAABSTRACT The vast majority of annotated transcripts in bacteria are mRNAs. Here we identify ~1,000 antisense transcripts in the model bacterium Escherichia coli. We propose that these transcripts are generated by promiscuous transcription initiation within genes and that many of them regulate expression of the overlapping gene. IMPORTANCE The vast majority of known genes in bacteria are protein coding, and there are very few known antisense transcripts within these genes, i.e., RNAs that are encoded opposite the gene. Here we demonstrate the existence of ~1,000 antisense RNAs in the model bacterium Escherichia coli. Given the high potential for these RNAs to base pair with mRNA of the overlapping gene and the likelihood of clashes between transcription complexes of antisense and sense transcripts, we propose that antisense RNAs represent an important but overlooked class of regulatory molecule.https://journals.asm.org/doi/10.1128/mBio.00024-10 |
| spellingShingle | James E. Dornenburg Anne M. DeVita Michael J. Palumbo Joseph T. Wade Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content> mBio |
| title | Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content> |
| title_full | Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content> |
| title_fullStr | Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content> |
| title_full_unstemmed | Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content> |
| title_short | Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content> |
| title_sort | widespread antisense transcription in named content content type genus species escherichia coli named content |
| url | https://journals.asm.org/doi/10.1128/mBio.00024-10 |
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