Effects of Cannabis Use on the Protein and Lipid Profile of Olfactory Neuroepithelium Cells from Schizophrenia Patients Studied by Synchrotron-Based FTIR Spectroscopy
Schizophrenia (SCZ) is a neurodevelopmental disorder with a high genetic component, but the presence of environmental stressors can be important for its onset and progression. Cannabis use can be a major risk factor for developing SCZ. However, despite the available data on the neurobiological under...
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| Format: | Article |
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MDPI AG
2020-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/2/329 |
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| author | Sergi Saladrigas-Manjón Tanja Dučić Liliana Galindo Cristina Fernández-Avilés Víctor Pérez Rafael de la Torre Patricia Robledo |
| author_facet | Sergi Saladrigas-Manjón Tanja Dučić Liliana Galindo Cristina Fernández-Avilés Víctor Pérez Rafael de la Torre Patricia Robledo |
| author_sort | Sergi Saladrigas-Manjón |
| collection | DOAJ |
| description | Schizophrenia (SCZ) is a neurodevelopmental disorder with a high genetic component, but the presence of environmental stressors can be important for its onset and progression. Cannabis use can be a major risk factor for developing SCZ. However, despite the available data on the neurobiological underpinnings of SCZ, there is an important lack of studies in human neuronal tissue and living cells addressing the effects of cannabis in SCZ patients. In this study, we analysed the most relevant bio-macromolecular constituents in olfactory neuroepithelium (ON) cells of healthy controls non-cannabis users, healthy cannabis users, SCZ patients non-cannabis users, and SCZ patients cannabis users using Synchrotron Radiation-Fourier Transform Infrared (SR-FTIR) spectrometry and microscopy. Our results revealed that SCZ patients non-cannabis users, and healthy cannabis users exhibit similar alterations in the macromolecular profile of ON cells, including disruption in lipid composition, increased lipid membrane renewal rate and lipid peroxidation, altered proteins containing more β-sheet structures, and showed an increase in DNA and histone methylation. Notably, these alterations were not observed in SCZ patients who use cannabis regularly. These data suggest a differential effect of cannabis in healthy controls and in SCZ patients in terms of the macromolecular constituents of ON cells. |
| first_indexed | 2024-12-12T13:18:01Z |
| format | Article |
| id | doaj.art-39d687919f834c62a493479c1801c693 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-12T13:18:01Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-39d687919f834c62a493479c1801c6932022-12-22T00:23:22ZengMDPI AGBiomolecules2218-273X2020-02-0110232910.3390/biom10020329biom10020329Effects of Cannabis Use on the Protein and Lipid Profile of Olfactory Neuroepithelium Cells from Schizophrenia Patients Studied by Synchrotron-Based FTIR SpectroscopySergi Saladrigas-Manjón0Tanja Dučić1Liliana Galindo2Cristina Fernández-Avilés3Víctor Pérez4Rafael de la Torre5Patricia Robledo6Integrative Pharmacology and Systems Neuroscience, IMIM-Hospital del Mar Research Institute, 08003 Barcelona, SpainALBA-CELLS Synchrotron, MIRAS Beamline, 08290 Cerdanyola del Vallès, SpainNeuropsychiatry and Addictions Institute (INAD) of Parc de Salut Mar, 08003 Barcelona, SpainIntegrative Pharmacology and Systems Neuroscience, IMIM-Hospital del Mar Research Institute, 08003 Barcelona, SpainNeuropsychiatry and Addictions Institute (INAD) of Parc de Salut Mar, 08003 Barcelona, SpainIntegrative Pharmacology and Systems Neuroscience, IMIM-Hospital del Mar Research Institute, 08003 Barcelona, SpainIntegrative Pharmacology and Systems Neuroscience, IMIM-Hospital del Mar Research Institute, 08003 Barcelona, SpainSchizophrenia (SCZ) is a neurodevelopmental disorder with a high genetic component, but the presence of environmental stressors can be important for its onset and progression. Cannabis use can be a major risk factor for developing SCZ. However, despite the available data on the neurobiological underpinnings of SCZ, there is an important lack of studies in human neuronal tissue and living cells addressing the effects of cannabis in SCZ patients. In this study, we analysed the most relevant bio-macromolecular constituents in olfactory neuroepithelium (ON) cells of healthy controls non-cannabis users, healthy cannabis users, SCZ patients non-cannabis users, and SCZ patients cannabis users using Synchrotron Radiation-Fourier Transform Infrared (SR-FTIR) spectrometry and microscopy. Our results revealed that SCZ patients non-cannabis users, and healthy cannabis users exhibit similar alterations in the macromolecular profile of ON cells, including disruption in lipid composition, increased lipid membrane renewal rate and lipid peroxidation, altered proteins containing more β-sheet structures, and showed an increase in DNA and histone methylation. Notably, these alterations were not observed in SCZ patients who use cannabis regularly. These data suggest a differential effect of cannabis in healthy controls and in SCZ patients in terms of the macromolecular constituents of ON cells.https://www.mdpi.com/2218-273X/10/2/329schizophreniaolfactory neuroepitheliumsr-ftircannabis |
| spellingShingle | Sergi Saladrigas-Manjón Tanja Dučić Liliana Galindo Cristina Fernández-Avilés Víctor Pérez Rafael de la Torre Patricia Robledo Effects of Cannabis Use on the Protein and Lipid Profile of Olfactory Neuroepithelium Cells from Schizophrenia Patients Studied by Synchrotron-Based FTIR Spectroscopy Biomolecules schizophrenia olfactory neuroepithelium sr-ftir cannabis |
| title | Effects of Cannabis Use on the Protein and Lipid Profile of Olfactory Neuroepithelium Cells from Schizophrenia Patients Studied by Synchrotron-Based FTIR Spectroscopy |
| title_full | Effects of Cannabis Use on the Protein and Lipid Profile of Olfactory Neuroepithelium Cells from Schizophrenia Patients Studied by Synchrotron-Based FTIR Spectroscopy |
| title_fullStr | Effects of Cannabis Use on the Protein and Lipid Profile of Olfactory Neuroepithelium Cells from Schizophrenia Patients Studied by Synchrotron-Based FTIR Spectroscopy |
| title_full_unstemmed | Effects of Cannabis Use on the Protein and Lipid Profile of Olfactory Neuroepithelium Cells from Schizophrenia Patients Studied by Synchrotron-Based FTIR Spectroscopy |
| title_short | Effects of Cannabis Use on the Protein and Lipid Profile of Olfactory Neuroepithelium Cells from Schizophrenia Patients Studied by Synchrotron-Based FTIR Spectroscopy |
| title_sort | effects of cannabis use on the protein and lipid profile of olfactory neuroepithelium cells from schizophrenia patients studied by synchrotron based ftir spectroscopy |
| topic | schizophrenia olfactory neuroepithelium sr-ftir cannabis |
| url | https://www.mdpi.com/2218-273X/10/2/329 |
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