Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid

Farahnaz Barahuie,1 Mohd Zobir Hussein,1 Shafinaz Abd Gani,2,3 Sharida Fakurazi,3,4 Zulkarnain Zainal11Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 2Department of Biochemistry, Faculty of Biotechnology and Biomolecular Scien...

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Main Authors: Barahuie F, Hussein MZ, Abd Gani S, Fakurazi S, Zainal Z
Format: Article
Language:English
Published: Dove Medical Press 2014-06-01
Series:International Journal of Nanomedicine
Online Access:http://www.dovepress.com/anticancer-nanodelivery-system-with-controlled-release-property-based--a17391
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author Barahuie F
Hussein MZ
Abd Gani S
Fakurazi S
Zainal Z
author_facet Barahuie F
Hussein MZ
Abd Gani S
Fakurazi S
Zainal Z
author_sort Barahuie F
collection DOAJ
description Farahnaz Barahuie,1 Mohd Zobir Hussein,1 Shafinaz Abd Gani,2,3 Sharida Fakurazi,3,4 Zulkarnain Zainal11Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 2Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 3Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 4Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, MalaysiaBackground: We characterize a novel nanocomposite that acts as an efficient anticancer agent. Methods: This nanocomposite consists of zinc layered hydroxide intercalated with protocatechuate (an anionic form of protocatechuic acid), that has been synthesized using a direct method with zinc oxide and protocatechuic acid as precursors.Results: The resulting protocatechuic acid nanocomposite (PAN) showed a basal spacing of 12.7 Å, indicating that protocatechuate was intercalated in a monolayer arrangement, with an angle of 54° from the Z-axis between the interlayers of the zinc layered hydroxide, and an estimated drug loading of about 35.7%. PAN exhibited the properties of a mesoporous type material, with greatly enhanced thermal stability of protocatechuate as compared to its free counterpart. The presence of protocatechuate in the interlayers of the zinc layered hydroxide was further supported by Fourier transform infrared spectroscopy. Protocatechuate was released from PAN in a slow and sustained manner. This mechanism of release was well represented by a pseudo-second order kinetics model. PAN has shown increased cytotoxicity compared to the free form of protocatechuic acid in all cancer cell lines tested. Tumor growth suppression was extensive, particularly in HepG2 and HT29 cell lines. Conclusion: PAN is suitable for use as a controlled release formulation, and our in vitro evidence indicates that PAN is an effective anticancer agent. PAN may have potential as a chemotherapeutic drug for human cancer.Keywords: protocatechuic acid, nanocomposite, zinc layered hydroxide, zinc oxide, 3T3, HepG2, HeLa, HT29
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spelling doaj.art-39d7cad72b3a4db39d8fa12063bb9e0a2022-12-21T20:03:35ZengDove Medical PressInternational Journal of Nanomedicine1178-20132014-06-012014Issue 13137314917391Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybridBarahuie FHussein MZAbd Gani SFakurazi SZainal ZFarahnaz Barahuie,1 Mohd Zobir Hussein,1 Shafinaz Abd Gani,2,3 Sharida Fakurazi,3,4 Zulkarnain Zainal11Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 2Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 3Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 4Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, MalaysiaBackground: We characterize a novel nanocomposite that acts as an efficient anticancer agent. Methods: This nanocomposite consists of zinc layered hydroxide intercalated with protocatechuate (an anionic form of protocatechuic acid), that has been synthesized using a direct method with zinc oxide and protocatechuic acid as precursors.Results: The resulting protocatechuic acid nanocomposite (PAN) showed a basal spacing of 12.7 Å, indicating that protocatechuate was intercalated in a monolayer arrangement, with an angle of 54° from the Z-axis between the interlayers of the zinc layered hydroxide, and an estimated drug loading of about 35.7%. PAN exhibited the properties of a mesoporous type material, with greatly enhanced thermal stability of protocatechuate as compared to its free counterpart. The presence of protocatechuate in the interlayers of the zinc layered hydroxide was further supported by Fourier transform infrared spectroscopy. Protocatechuate was released from PAN in a slow and sustained manner. This mechanism of release was well represented by a pseudo-second order kinetics model. PAN has shown increased cytotoxicity compared to the free form of protocatechuic acid in all cancer cell lines tested. Tumor growth suppression was extensive, particularly in HepG2 and HT29 cell lines. Conclusion: PAN is suitable for use as a controlled release formulation, and our in vitro evidence indicates that PAN is an effective anticancer agent. PAN may have potential as a chemotherapeutic drug for human cancer.Keywords: protocatechuic acid, nanocomposite, zinc layered hydroxide, zinc oxide, 3T3, HepG2, HeLa, HT29http://www.dovepress.com/anticancer-nanodelivery-system-with-controlled-release-property-based--a17391
spellingShingle Barahuie F
Hussein MZ
Abd Gani S
Fakurazi S
Zainal Z
Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
International Journal of Nanomedicine
title Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
title_full Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
title_fullStr Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
title_full_unstemmed Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
title_short Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
title_sort anticancer nanodelivery system with controlled release property based on protocatechuate ndash zinc layered hydroxide nanohybrid
url http://www.dovepress.com/anticancer-nanodelivery-system-with-controlled-release-property-based--a17391
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AT husseinmz anticancernanodeliverysystemwithcontrolledreleasepropertybasedonprotocatechuatendashzinclayeredhydroxidenanohybrid
AT abdganis anticancernanodeliverysystemwithcontrolledreleasepropertybasedonprotocatechuatendashzinclayeredhydroxidenanohybrid
AT fakurazis anticancernanodeliverysystemwithcontrolledreleasepropertybasedonprotocatechuatendashzinclayeredhydroxidenanohybrid
AT zainalz anticancernanodeliverysystemwithcontrolledreleasepropertybasedonprotocatechuatendashzinclayeredhydroxidenanohybrid