Analysis of the LacI Family Regulators of Erwinia chrysanthemi 3937, Involvement in the Bacterial Phytopathogenicity
Analysis of the regulators of the LacI family was performed in order to identify those potentially involved in pathogenicity of Erwinia chrysanthemi (Dickeya dadantii). Among the 18 members of the LacI family, the function of 11 members is either known or predicted and only 7 members have, as yet, n...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2008-11-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-11-1471 |
| _version_ | 1811291319531732992 |
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| author | Frédérique Van Gijsegem Aleksandra Wlodarczyk Amandine Cornu Sylvie Reverchon Nicole Hugouvieux-Cotte-Pattat |
| author_facet | Frédérique Van Gijsegem Aleksandra Wlodarczyk Amandine Cornu Sylvie Reverchon Nicole Hugouvieux-Cotte-Pattat |
| author_sort | Frédérique Van Gijsegem |
| collection | DOAJ |
| description | Analysis of the regulators of the LacI family was performed in order to identify those potentially involved in pathogenicity of Erwinia chrysanthemi (Dickeya dadantii). Among the 18 members of the LacI family, the function of 11 members is either known or predicted and only 7 members have, as yet, no proposed function. Inactivation of these seven genes, called lfaR, lfbR, lfcR, lfdR, lfeR, lffR, and lfgR, demonstrated that four of them are important for plant infection. The lfaR and lfcR mutants showed a reduced virulence on chicory, Saintpaulia sp., and Arabidopsis. The lfeR mutant showed a reduced virulence on Arabidopsis. The lfdR mutant was more efficient than the wild-type strain in initiating maceration on Saintpaulia sp. The genetic environment of each regulator was examined to detect adjacent genes potentially involved in a common function. Construction of transcriptional fusions in these neighboring genes demonstrated that five regulators, LfaR, LfcR, LfeR, LffR, and LfgR, act as repressors of adjacent genes. Analysis of these fusions also indicated that the genes controlled by LfaR, LfcR, LfgR, and LffR are expressed during plant infection. Moreover, addition of crude plant extracts to culture medium demonstrated that the expression of the LfaR- and LfgR-controlled genes is specifically induced by plant components. |
| first_indexed | 2024-04-13T04:27:30Z |
| format | Article |
| id | doaj.art-39d8ed052a744528b7be2dc41074f6fc |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:27:30Z |
| publishDate | 2008-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-39d8ed052a744528b7be2dc41074f6fc2022-12-22T03:02:26ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062008-11-0121111471148110.1094/MPMI-21-11-1471Analysis of the LacI Family Regulators of Erwinia chrysanthemi 3937, Involvement in the Bacterial PhytopathogenicityFrédérique Van GijsegemAleksandra WlodarczykAmandine CornuSylvie ReverchonNicole Hugouvieux-Cotte-PattatAnalysis of the regulators of the LacI family was performed in order to identify those potentially involved in pathogenicity of Erwinia chrysanthemi (Dickeya dadantii). Among the 18 members of the LacI family, the function of 11 members is either known or predicted and only 7 members have, as yet, no proposed function. Inactivation of these seven genes, called lfaR, lfbR, lfcR, lfdR, lfeR, lffR, and lfgR, demonstrated that four of them are important for plant infection. The lfaR and lfcR mutants showed a reduced virulence on chicory, Saintpaulia sp., and Arabidopsis. The lfeR mutant showed a reduced virulence on Arabidopsis. The lfdR mutant was more efficient than the wild-type strain in initiating maceration on Saintpaulia sp. The genetic environment of each regulator was examined to detect adjacent genes potentially involved in a common function. Construction of transcriptional fusions in these neighboring genes demonstrated that five regulators, LfaR, LfcR, LfeR, LffR, and LfgR, act as repressors of adjacent genes. Analysis of these fusions also indicated that the genes controlled by LfaR, LfcR, LfgR, and LffR are expressed during plant infection. Moreover, addition of crude plant extracts to culture medium demonstrated that the expression of the LfaR- and LfgR-controlled genes is specifically induced by plant components.https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-11-1471inductionin planta expressionregulation |
| spellingShingle | Frédérique Van Gijsegem Aleksandra Wlodarczyk Amandine Cornu Sylvie Reverchon Nicole Hugouvieux-Cotte-Pattat Analysis of the LacI Family Regulators of Erwinia chrysanthemi 3937, Involvement in the Bacterial Phytopathogenicity Molecular Plant-Microbe Interactions induction in planta expression regulation |
| title | Analysis of the LacI Family Regulators of Erwinia chrysanthemi 3937, Involvement in the Bacterial Phytopathogenicity |
| title_full | Analysis of the LacI Family Regulators of Erwinia chrysanthemi 3937, Involvement in the Bacterial Phytopathogenicity |
| title_fullStr | Analysis of the LacI Family Regulators of Erwinia chrysanthemi 3937, Involvement in the Bacterial Phytopathogenicity |
| title_full_unstemmed | Analysis of the LacI Family Regulators of Erwinia chrysanthemi 3937, Involvement in the Bacterial Phytopathogenicity |
| title_short | Analysis of the LacI Family Regulators of Erwinia chrysanthemi 3937, Involvement in the Bacterial Phytopathogenicity |
| title_sort | analysis of the laci family regulators of erwinia chrysanthemi 3937 involvement in the bacterial phytopathogenicity |
| topic | induction in planta expression regulation |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-11-1471 |
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