Effect of Propolis on Precocious Puberty in Female Rats
INTRODUCTION: Nutrition and exposure to various chemicals, including environmental pollution, insecticides, and plant phytoestrogens (having oestrogen-like effects), are environmental factors that affect puberty onset. The aim of this study was to identify the effects of propolis, which has been rep...
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| Format: | Article |
| Language: | English |
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Galenos Yayincilik
2022-12-01
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| Series: | JCRPE |
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| Online Access: | https://jcrpe.org/jvi.aspx?pdir=jcrpe&plng=eng&un=JCRPE-97658&look4= |
| _version_ | 1797921219942547456 |
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| author | Recep Polat Erdem Çokluk Özcan Budak Fatıma Betül Tuncer |
| author_facet | Recep Polat Erdem Çokluk Özcan Budak Fatıma Betül Tuncer |
| author_sort | Recep Polat |
| collection | DOAJ |
| description | INTRODUCTION: Nutrition and exposure to various chemicals, including environmental pollution, insecticides, and plant phytoestrogens (having oestrogen-like effects), are environmental factors that affect puberty onset. The aim of this study was to identify the effects of propolis, which has been reported to have oestrogenic effects, on precocious puberty and the reproductive system in prepubertal female rats (ovary, endometrium, breast).
METHODS: Thirty-four 25-day-old, prepubertal, female Sprague-Dawley rats were included. Rats were randomly divided into the propolis (n=17) and control groups (n=17). The primary endpoint was the number of rats that developed vaginal opening, a sign of puberty, at 12-day follow-up. In addition, the effect of propolis on ovary, uterus and breast tissue was evaluated histologically.
RESULTS: Vaginal patency occurred earlier (about 7.5 days sooner) in the propolis group and all animals in the propolis group had vaginal patency by day 12. The number of ovarian follicles (in all follicles), endometrial thickness, and mammary gland secretory gland area were significantly higher in the propolis group than in the control group (all p<0.001). In addition, Ki-67 activity in the endometrium, breast tissue and ovary was more intense in the propolis group compared to the control group (all p<0.001).
DISCUSSION AND CONCLUSION: Propolis triggers precocious puberty in female rats, possibly by interacting with the oestrogen receptor. The mechanism of action of propolis should be considered before prescribing it. In addition, further studies are needed to explore the mechanism of action of propolis and to determine the component of propolis that triggers puberty. |
| first_indexed | 2024-04-10T14:13:04Z |
| format | Article |
| id | doaj.art-39d93008b4d747cf8da88ff7feb88361 |
| institution | Directory Open Access Journal |
| issn | 1308-5727 1308-5735 |
| language | English |
| last_indexed | 2024-04-10T14:13:04Z |
| publishDate | 2022-12-01 |
| publisher | Galenos Yayincilik |
| record_format | Article |
| series | JCRPE |
| spelling | doaj.art-39d93008b4d747cf8da88ff7feb883612023-02-15T16:09:38ZengGalenos YayincilikJCRPE1308-57271308-57352022-12-0114441542110.4274/jcrpe.galenos.2022.2022-1-18Effect of Propolis on Precocious Puberty in Female RatsRecep Polat0https://orcid.org/0000-0002-3786-0739Erdem Çokluk1https://orcid.org/0000-0002-6205-5109Özcan Budak2https://orcid.org/0000-0002-2617-3175Fatıma Betül Tuncer3https://orcid.org/0000-0002-4034-4188Sakarya University, Sakarya Training and Research Hospital, Clinic of Pediatric Endocrinology, Sakarya, TurkeySakarya University Faculty of Medicine, Department of Medical Biochemistry, Sakarya, TurkeySakarya University Faculty of Medicine, Department of Histology-Embryology, Sakarya, TurkeySakarya University, Sakarya Training and Research Hospital, Clinic of Medical Biochemistry, Sakarya, TurkeyINTRODUCTION: Nutrition and exposure to various chemicals, including environmental pollution, insecticides, and plant phytoestrogens (having oestrogen-like effects), are environmental factors that affect puberty onset. The aim of this study was to identify the effects of propolis, which has been reported to have oestrogenic effects, on precocious puberty and the reproductive system in prepubertal female rats (ovary, endometrium, breast). METHODS: Thirty-four 25-day-old, prepubertal, female Sprague-Dawley rats were included. Rats were randomly divided into the propolis (n=17) and control groups (n=17). The primary endpoint was the number of rats that developed vaginal opening, a sign of puberty, at 12-day follow-up. In addition, the effect of propolis on ovary, uterus and breast tissue was evaluated histologically. RESULTS: Vaginal patency occurred earlier (about 7.5 days sooner) in the propolis group and all animals in the propolis group had vaginal patency by day 12. The number of ovarian follicles (in all follicles), endometrial thickness, and mammary gland secretory gland area were significantly higher in the propolis group than in the control group (all p<0.001). In addition, Ki-67 activity in the endometrium, breast tissue and ovary was more intense in the propolis group compared to the control group (all p<0.001). DISCUSSION AND CONCLUSION: Propolis triggers precocious puberty in female rats, possibly by interacting with the oestrogen receptor. The mechanism of action of propolis should be considered before prescribing it. In addition, further studies are needed to explore the mechanism of action of propolis and to determine the component of propolis that triggers puberty.https://jcrpe.org/jvi.aspx?pdir=jcrpe&plng=eng&un=JCRPE-97658&look4=phytoestrogenspropolisprecocious pubertyrat |
| spellingShingle | Recep Polat Erdem Çokluk Özcan Budak Fatıma Betül Tuncer Effect of Propolis on Precocious Puberty in Female Rats JCRPE phytoestrogens propolis precocious puberty rat |
| title | Effect of Propolis on Precocious Puberty in Female Rats |
| title_full | Effect of Propolis on Precocious Puberty in Female Rats |
| title_fullStr | Effect of Propolis on Precocious Puberty in Female Rats |
| title_full_unstemmed | Effect of Propolis on Precocious Puberty in Female Rats |
| title_short | Effect of Propolis on Precocious Puberty in Female Rats |
| title_sort | effect of propolis on precocious puberty in female rats |
| topic | phytoestrogens propolis precocious puberty rat |
| url | https://jcrpe.org/jvi.aspx?pdir=jcrpe&plng=eng&un=JCRPE-97658&look4= |
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