NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of <i>Vibrio diabolicus</i> CNCM I-1629
Diabolican, or HE800, is an exopolysaccharide secreted by the non-pathogenic Gram-negative marine bacterium <i>Vibrio diabolicus</i> (CNCM I-1629). This polysaccharide was enzymatically degraded by the <i>Bacteroides cellulosilyticus</i> WH2 hyaluronan lyase. The end products...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/20/12/731 |
| _version_ | 1797456579078914048 |
|---|---|
| author | Sophie Drouillard Laurent Poulet Claire Boisset Christine Delbarre-Ladrat William Helbert |
| author_facet | Sophie Drouillard Laurent Poulet Claire Boisset Christine Delbarre-Ladrat William Helbert |
| author_sort | Sophie Drouillard |
| collection | DOAJ |
| description | Diabolican, or HE800, is an exopolysaccharide secreted by the non-pathogenic Gram-negative marine bacterium <i>Vibrio diabolicus</i> (CNCM I-1629). This polysaccharide was enzymatically degraded by the <i>Bacteroides cellulosilyticus</i> WH2 hyaluronan lyase. The end products were purified by size-exclusion chromatography and their structures were analyzed in depth by nuclear magnetic resonance (NMR). The oligosaccharide structures confirmed the possible site of cleavage of the enzyme showing plasticity in the substrate recognitions. The production of glycosaminoglycan-mimetic oligosaccharides of defined molecular weight and structure opens new perspectives in the valorization of the marine polysaccharide diabolican. |
| first_indexed | 2024-03-09T16:09:50Z |
| format | Article |
| id | doaj.art-39d9bd8eb5364fdc8e9b028dcfe25488 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T16:09:50Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-39d9bd8eb5364fdc8e9b028dcfe254882023-11-24T16:19:12ZengMDPI AGMarine Drugs1660-33972022-11-01201273110.3390/md20120731NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of <i>Vibrio diabolicus</i> CNCM I-1629Sophie Drouillard0Laurent Poulet1Claire Boisset2Christine Delbarre-Ladrat3William Helbert4CERMAV, CNRS and Grenoble Alpes Université, 38000 Grenoble, FranceCERMAV, CNRS and Grenoble Alpes Université, 38000 Grenoble, FranceCERMAV, CNRS and Grenoble Alpes Université, 38000 Grenoble, FranceIfremer, MASAE, EM3B Laboratory, F-44300 Nantes, FranceCERMAV, CNRS and Grenoble Alpes Université, 38000 Grenoble, FranceDiabolican, or HE800, is an exopolysaccharide secreted by the non-pathogenic Gram-negative marine bacterium <i>Vibrio diabolicus</i> (CNCM I-1629). This polysaccharide was enzymatically degraded by the <i>Bacteroides cellulosilyticus</i> WH2 hyaluronan lyase. The end products were purified by size-exclusion chromatography and their structures were analyzed in depth by nuclear magnetic resonance (NMR). The oligosaccharide structures confirmed the possible site of cleavage of the enzyme showing plasticity in the substrate recognitions. The production of glycosaminoglycan-mimetic oligosaccharides of defined molecular weight and structure opens new perspectives in the valorization of the marine polysaccharide diabolican.https://www.mdpi.com/1660-3397/20/12/731exopolysaccharidediabolicanhyaluronan lyaseNMR |
| spellingShingle | Sophie Drouillard Laurent Poulet Claire Boisset Christine Delbarre-Ladrat William Helbert NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of <i>Vibrio diabolicus</i> CNCM I-1629 Marine Drugs exopolysaccharide diabolican hyaluronan lyase NMR |
| title | NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of <i>Vibrio diabolicus</i> CNCM I-1629 |
| title_full | NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of <i>Vibrio diabolicus</i> CNCM I-1629 |
| title_fullStr | NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of <i>Vibrio diabolicus</i> CNCM I-1629 |
| title_full_unstemmed | NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of <i>Vibrio diabolicus</i> CNCM I-1629 |
| title_short | NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of <i>Vibrio diabolicus</i> CNCM I-1629 |
| title_sort | nmr analyses of the enzymatic degradation end products of diabolican the secreted eps of i vibrio diabolicus i cncm i 1629 |
| topic | exopolysaccharide diabolican hyaluronan lyase NMR |
| url | https://www.mdpi.com/1660-3397/20/12/731 |
| work_keys_str_mv | AT sophiedrouillard nmranalysesoftheenzymaticdegradationendproductsofdiabolicanthesecretedepsofivibriodiabolicusicncmi1629 AT laurentpoulet nmranalysesoftheenzymaticdegradationendproductsofdiabolicanthesecretedepsofivibriodiabolicusicncmi1629 AT claireboisset nmranalysesoftheenzymaticdegradationendproductsofdiabolicanthesecretedepsofivibriodiabolicusicncmi1629 AT christinedelbarreladrat nmranalysesoftheenzymaticdegradationendproductsofdiabolicanthesecretedepsofivibriodiabolicusicncmi1629 AT williamhelbert nmranalysesoftheenzymaticdegradationendproductsofdiabolicanthesecretedepsofivibriodiabolicusicncmi1629 |