Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice.
A single-injection vaccine formulation that provides for both a prime and a boost immunization would have various advantages over a multiple-injection regime. For such a vaccine formulation, it is essential that the booster dose is released after a certain, preferably adjustable, lag time. In this s...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6117011?pdf=render |
| _version_ | 1819072936199323648 |
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| author | Katie Amssoms Philip A Born Max Beugeling Ben De Clerck Ellen Van Gulck Wouter L J Hinrichs Henderik W Frijlink Niels Grasmeijer Guenter Kraus Roger Sutmuller Kenny Simmen Lieven Baert |
| author_facet | Katie Amssoms Philip A Born Max Beugeling Ben De Clerck Ellen Van Gulck Wouter L J Hinrichs Henderik W Frijlink Niels Grasmeijer Guenter Kraus Roger Sutmuller Kenny Simmen Lieven Baert |
| author_sort | Katie Amssoms |
| collection | DOAJ |
| description | A single-injection vaccine formulation that provides for both a prime and a boost immunization would have various advantages over a multiple-injection regime. For such a vaccine formulation, it is essential that the booster dose is released after a certain, preferably adjustable, lag time. In this study we investigated whether a core-shell based implant, containing ovalbumin as core material and poly(DL-lactic-co-glycolic acid) of various monomer ratios as shell material can be used to obtain such a booster release. An in vitro release study showed that the lag time after which the ovalbumin was released from the core-shell implant increased with increasing lactic to glycolic acid ratio of the polymer and ranged from 3-6 weeks. Fluorescence spectroscopy showed minimal differences between native ovalbumin and ovalbumin from core-shell implants that were incubated until just before the observed in vitro release. In addition, mice immunized with a subcutaneous inserted core-shell implant containing ovalbumin showed an ovalbumin-specific IgG1 antibody response after a lag time of 4 or 6-8 weeks. Moreover, delayed release of ovalbumin caused higher IgG1 antibody titers than conventional subcutaneous vaccination with ovalbumin dissolved in PBS. Collectively, these findings could contribute to the further development of a single-injection vaccine, making multiple injections of the vaccine superfluous. |
| first_indexed | 2024-12-21T17:45:38Z |
| format | Article |
| id | doaj.art-39d9cf7d09974343b19898981fbcf509 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T17:45:38Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-39d9cf7d09974343b19898981fbcf5092022-12-21T18:55:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01138e020296110.1371/journal.pone.0202961Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice.Katie AmssomsPhilip A BornMax BeugelingBen De ClerckEllen Van GulckWouter L J HinrichsHenderik W FrijlinkNiels GrasmeijerGuenter KrausRoger SutmullerKenny SimmenLieven BaertA single-injection vaccine formulation that provides for both a prime and a boost immunization would have various advantages over a multiple-injection regime. For such a vaccine formulation, it is essential that the booster dose is released after a certain, preferably adjustable, lag time. In this study we investigated whether a core-shell based implant, containing ovalbumin as core material and poly(DL-lactic-co-glycolic acid) of various monomer ratios as shell material can be used to obtain such a booster release. An in vitro release study showed that the lag time after which the ovalbumin was released from the core-shell implant increased with increasing lactic to glycolic acid ratio of the polymer and ranged from 3-6 weeks. Fluorescence spectroscopy showed minimal differences between native ovalbumin and ovalbumin from core-shell implants that were incubated until just before the observed in vitro release. In addition, mice immunized with a subcutaneous inserted core-shell implant containing ovalbumin showed an ovalbumin-specific IgG1 antibody response after a lag time of 4 or 6-8 weeks. Moreover, delayed release of ovalbumin caused higher IgG1 antibody titers than conventional subcutaneous vaccination with ovalbumin dissolved in PBS. Collectively, these findings could contribute to the further development of a single-injection vaccine, making multiple injections of the vaccine superfluous.http://europepmc.org/articles/PMC6117011?pdf=render |
| spellingShingle | Katie Amssoms Philip A Born Max Beugeling Ben De Clerck Ellen Van Gulck Wouter L J Hinrichs Henderik W Frijlink Niels Grasmeijer Guenter Kraus Roger Sutmuller Kenny Simmen Lieven Baert Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice. PLoS ONE |
| title | Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice. |
| title_full | Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice. |
| title_fullStr | Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice. |
| title_full_unstemmed | Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice. |
| title_short | Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice. |
| title_sort | ovalbumin containing core shell implants suitable to obtain a delayed igg1 antibody response in support of a biphasic pulsatile release profile in mice |
| url | http://europepmc.org/articles/PMC6117011?pdf=render |
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