Effects of steroid hormones on epithelial and stromal cells culture and proliferation of laying hens, in vitro
The aim of this study was to study the effect of steroid hormones on the morphology, growth and proliferation of endometrial cells of laying hens in vitro. Endometrial epithelial and stromal cells were isolated and cultured via type I collagenase digestion, which then were identified by using kerati...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2018-03-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2351 |
| _version_ | 1797800629817573376 |
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| author | Jie SUN Yourong YE Herong LIAO Lei ZHANG Heng YANG |
| author_facet | Jie SUN Yourong YE Herong LIAO Lei ZHANG Heng YANG |
| author_sort | Jie SUN |
| collection | DOAJ |
| description | The aim of this study was to study the effect of steroid hormones on the morphology, growth and proliferation of endometrial cells of laying
hens in vitro. Endometrial epithelial and stromal cells were isolated and cultured via type I collagenase digestion, which then were identified
by using keratin CK18 or vimentin by immunohistochemistry and treated with steroid hormones, such as E2 and P4, and the growth curves
in vitro evaluated by MTT assay. The results showed that endometrial epithelial and stromal cells were successfully isolated and cultured, both
of which began to enter the logarithmic phase of growth after 24 h and reached the plateau phase at 7 days after inoculation. Compared to
control group, the endometrial epithelial cells were significantly proliferated (P<0.05) by treating with E2 (100 nmol/L) and P4 (100 nmol/L),
however the stromal cells were only significantly proliferated (P<0.05) by treating with P4 (100 nmol/L). From these results we can suppose
that steroid hormones could positively effect on the proliferation of endometrial cells of laying hens, which would contribute to explore a
method to examine the most suitable conditions for endometrial culture in vitro and help to improve further studies on the physiological and
functional characteristics of the uterus in the poultry. |
| first_indexed | 2024-03-13T04:37:09Z |
| format | Article |
| id | doaj.art-39da3b9f0d2f48ccb177b7b3f94ee845 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:09Z |
| publishDate | 2018-03-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-39da3b9f0d2f48ccb177b7b3f94ee8452023-06-19T06:43:42ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512018-03-0124340140710.9775/kvfd.2017.190552351Effects of steroid hormones on epithelial and stromal cells culture and proliferation of laying hens, in vitroJie SUN0Yourong YE1Herong LIAO2Lei ZHANG3Heng YANG4College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINAThe aim of this study was to study the effect of steroid hormones on the morphology, growth and proliferation of endometrial cells of laying hens in vitro. Endometrial epithelial and stromal cells were isolated and cultured via type I collagenase digestion, which then were identified by using keratin CK18 or vimentin by immunohistochemistry and treated with steroid hormones, such as E2 and P4, and the growth curves in vitro evaluated by MTT assay. The results showed that endometrial epithelial and stromal cells were successfully isolated and cultured, both of which began to enter the logarithmic phase of growth after 24 h and reached the plateau phase at 7 days after inoculation. Compared to control group, the endometrial epithelial cells were significantly proliferated (P<0.05) by treating with E2 (100 nmol/L) and P4 (100 nmol/L), however the stromal cells were only significantly proliferated (P<0.05) by treating with P4 (100 nmol/L). From these results we can suppose that steroid hormones could positively effect on the proliferation of endometrial cells of laying hens, which would contribute to explore a method to examine the most suitable conditions for endometrial culture in vitro and help to improve further studies on the physiological and functional characteristics of the uterus in the poultry.https://vetdergikafkas.org/pdf.php?id=2351endometriumepithelium celllaying hensex hormonein vitro |
| spellingShingle | Jie SUN Yourong YE Herong LIAO Lei ZHANG Heng YANG Effects of steroid hormones on epithelial and stromal cells culture and proliferation of laying hens, in vitro Kafkas Universitesi Veteriner Fakültesi Dergisi endometrium epithelium cell laying hen sex hormone in vitro |
| title | Effects of steroid hormones on epithelial and stromal cells culture and proliferation of laying hens, in vitro |
| title_full | Effects of steroid hormones on epithelial and stromal cells culture and proliferation of laying hens, in vitro |
| title_fullStr | Effects of steroid hormones on epithelial and stromal cells culture and proliferation of laying hens, in vitro |
| title_full_unstemmed | Effects of steroid hormones on epithelial and stromal cells culture and proliferation of laying hens, in vitro |
| title_short | Effects of steroid hormones on epithelial and stromal cells culture and proliferation of laying hens, in vitro |
| title_sort | effects of steroid hormones on epithelial and stromal cells culture and proliferation of laying hens in vitro |
| topic | endometrium epithelium cell laying hen sex hormone in vitro |
| url | https://vetdergikafkas.org/pdf.php?id=2351 |
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