Determination of flavonolignans of dried fruits of Silybum marianum )L.( Gaertn. collected from different areas of Iran by spectrophotometry, TLC and HPLC
Background: Silymarin complex consisted of five flavonolignans (silybin A & B, Isosylibin A & B, silychristin, silydianin and taxifolin) isolated from dried fruiets of Silybum marianum (L.) Gaertn. Silybin is the main component of silymarin complex. Hepatoprotective activeity of silymarin ma...
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2005-02-01
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| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-829-en.html |
| _version_ | 1819123280350543872 |
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| author | T Hasanloo R Khavari-Negad E Majidi SA Ziai MR Shams- Ardekani |
| author_facet | T Hasanloo R Khavari-Negad E Majidi SA Ziai MR Shams- Ardekani |
| author_sort | T Hasanloo |
| collection | DOAJ |
| description | Background: Silymarin complex consisted of five flavonolignans (silybin A & B, Isosylibin A & B, silychristin, silydianin and taxifolin) isolated from dried fruiets of Silybum marianum (L.) Gaertn. Silybin is the main component of silymarin complex. Hepatoprotective activeity of silymarin may related to its antioxidant property. Objective: This study was conducted to understand the environmental condition on silymarin methabolism. Methods: Silymarin extraction and flavolignans assay by spectrophotometery, TLC and HPLC was performed on Milk thistle seeds collected from different areas of Iran, Hungarian seeds cultivated in greenhouse and field in karaj. Results: Valasht and Borazjan had the highest levels of silymain content by spectrophotometery method. In TLC method all 5 components were identified. We analyzed the flavolignans contents by HPLC and results showed that Borazjan had the highest level of silymarin. Conclusion: To produce commercially silymarin we need to search for the best genotype. |
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| format | Article |
| id | doaj.art-39db0a08a31e441884996e8eb42b03b8 |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-22T07:05:50Z |
| publishDate | 2005-02-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-39db0a08a31e441884996e8eb42b03b82022-12-21T18:34:41ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582005-02-014132532Determination of flavonolignans of dried fruits of Silybum marianum )L.( Gaertn. collected from different areas of Iran by spectrophotometry, TLC and HPLCT Hasanloo0R Khavari-Negad1E Majidi2SA Ziai3MR Shams- Ardekani4 1- Faculty of science, Teacher Training University, Tehran, Iran 2- Faculty of science, Teacher Training University, Tehran, Iran 3- Department of physiology, Agricultural Biotechnology Research Institute of Iran (ABRII), Karadj, Iran 4- Department of pharmacology, Institute of Medicinal Plants ACECR 5- Faculty of pharmacology, Tehran University, Karadj, Iran Background: Silymarin complex consisted of five flavonolignans (silybin A & B, Isosylibin A & B, silychristin, silydianin and taxifolin) isolated from dried fruiets of Silybum marianum (L.) Gaertn. Silybin is the main component of silymarin complex. Hepatoprotective activeity of silymarin may related to its antioxidant property. Objective: This study was conducted to understand the environmental condition on silymarin methabolism. Methods: Silymarin extraction and flavolignans assay by spectrophotometery, TLC and HPLC was performed on Milk thistle seeds collected from different areas of Iran, Hungarian seeds cultivated in greenhouse and field in karaj. Results: Valasht and Borazjan had the highest levels of silymain content by spectrophotometery method. In TLC method all 5 components were identified. We analyzed the flavolignans contents by HPLC and results showed that Borazjan had the highest level of silymarin. Conclusion: To produce commercially silymarin we need to search for the best genotype.http://jmp.ir/article-1-829-en.htmlflavonolignanssilybinsilymarinsilybum marianum l. |
| spellingShingle | T Hasanloo R Khavari-Negad E Majidi SA Ziai MR Shams- Ardekani Determination of flavonolignans of dried fruits of Silybum marianum )L.( Gaertn. collected from different areas of Iran by spectrophotometry, TLC and HPLC Journal of Medicinal Plants flavonolignans silybin silymarin silybum marianum l. |
| title | Determination of flavonolignans of dried fruits of Silybum marianum )L.( Gaertn. collected from different areas of Iran by spectrophotometry, TLC and HPLC |
| title_full | Determination of flavonolignans of dried fruits of Silybum marianum )L.( Gaertn. collected from different areas of Iran by spectrophotometry, TLC and HPLC |
| title_fullStr | Determination of flavonolignans of dried fruits of Silybum marianum )L.( Gaertn. collected from different areas of Iran by spectrophotometry, TLC and HPLC |
| title_full_unstemmed | Determination of flavonolignans of dried fruits of Silybum marianum )L.( Gaertn. collected from different areas of Iran by spectrophotometry, TLC and HPLC |
| title_short | Determination of flavonolignans of dried fruits of Silybum marianum )L.( Gaertn. collected from different areas of Iran by spectrophotometry, TLC and HPLC |
| title_sort | determination of flavonolignans of dried fruits of silybum marianum l gaertn collected from different areas of iran by spectrophotometry tlc and hplc |
| topic | flavonolignans silybin silymarin silybum marianum l. |
| url | http://jmp.ir/article-1-829-en.html |
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