Inhibition study of biofilms production of Pseudomonas aeruginosa strains in the presence of propolis
One of the main failures of antibiotics treatments of P. aeruginosa infections is due to the production of biofilms. The objective of this work was to study the inhibition of biofilm production in Pseudomonas aeruginosa strains in the presence of propolis. Susceptibility testing and synergism were p...
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| Format: | Article |
| Language: | English |
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2020-06-01
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| Series: | Kasmera |
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| Online Access: | http://produccioncientificaluz.org/index.php/kasmera/article/view/30834 |
| _version_ | 1797716858038648832 |
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| author | Lorena Abadía-Patiño Carlos Enrique Bravo-Rodriguez José Luís Prin Saúl José Mosqueda-Paraqueimo |
| author_facet | Lorena Abadía-Patiño Carlos Enrique Bravo-Rodriguez José Luís Prin Saúl José Mosqueda-Paraqueimo |
| author_sort | Lorena Abadía-Patiño |
| collection | DOAJ |
| description | One of the main failures of antibiotics treatments of P. aeruginosa infections is due to the production of biofilms. The objective of this work was to study the inhibition of biofilm production in Pseudomonas aeruginosa strains in the presence of propolis. Susceptibility testing and synergism were performed in all strains. Biofilm production level was determined onto nitrocellulose membranes and transmission electronic microscopy. Biofilm inhibition propolis was did on plate dilution. “Dr. Julio Rodríguez” hospital, in Cumaná, P. aeruginosa strains were isolated from different infectious processes, mainly diabetic foot. All the strains produced biofilm at different levels (60% mild, 24% moderate and 16% high). Sixty percent of the strains are producers of MBL, and 49% of AmpC. The predominant antibiotype was resistance to aminoglycosides and fluoroquinolones (XII). Seven strains were resistant’s to colistin. Biofilms treated with propolis (10 μg/ml) were almost completely inhibited. In conclusion, the propolis achieved to eradicate the in vitro biofilm production, breaking the barriers of the different mechanisms of resistance to the antibiotics expressed by the P. aeruginosa strains studied in this work |
| first_indexed | 2024-03-12T08:27:34Z |
| format | Article |
| id | doaj.art-39db59eaf65f4065b83af3ca8e3e4180 |
| institution | Directory Open Access Journal |
| issn | 0075-5222 2477-9628 |
| language | English |
| last_indexed | 2024-03-12T08:27:34Z |
| publishDate | 2020-06-01 |
| publisher | Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
| record_format | Article |
| series | Kasmera |
| spelling | doaj.art-39db59eaf65f4065b83af3ca8e3e41802023-09-02T17:59:14ZengUniversidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y TropicalesKasmera0075-52222477-96282020-06-01481e48130843e4813084310.5281/zenodo.387064332076Inhibition study of biofilms production of Pseudomonas aeruginosa strains in the presence of propolisLorena Abadía-Patiño0Carlos Enrique Bravo-Rodriguez1José Luís Prin2Saúl José Mosqueda-Paraqueimo3Universidad de Oriente. Núcleo Sucre. Instituto de Investigaciones en Biomedicina y Ciencias Aplicadas. Departamento de Biomedicina. Departamento de Bioanálisis. Laboratorio de Resistencia Bacteriana. Cumaná-SucreUniversidad de Oriente. Núcleo Sucre. Departamento de Bioanálisis. Cumaná-Sucre.Universidad de Oriente. Núcleo Sucre. Instituto de Investigaciones en Biomedicina y Ciencias Aplicadas. Departamento de Ciencias de los Materiales. Cumaná-SucreUniversidad de Oriente. Núcleo Sucre. Departamento de Matemáticas. Cumaná-Sucre. VenezuelaOne of the main failures of antibiotics treatments of P. aeruginosa infections is due to the production of biofilms. The objective of this work was to study the inhibition of biofilm production in Pseudomonas aeruginosa strains in the presence of propolis. Susceptibility testing and synergism were performed in all strains. Biofilm production level was determined onto nitrocellulose membranes and transmission electronic microscopy. Biofilm inhibition propolis was did on plate dilution. “Dr. Julio Rodríguez” hospital, in Cumaná, P. aeruginosa strains were isolated from different infectious processes, mainly diabetic foot. All the strains produced biofilm at different levels (60% mild, 24% moderate and 16% high). Sixty percent of the strains are producers of MBL, and 49% of AmpC. The predominant antibiotype was resistance to aminoglycosides and fluoroquinolones (XII). Seven strains were resistant’s to colistin. Biofilms treated with propolis (10 μg/ml) were almost completely inhibited. In conclusion, the propolis achieved to eradicate the in vitro biofilm production, breaking the barriers of the different mechanisms of resistance to the antibiotics expressed by the P. aeruginosa strains studied in this workhttp://produccioncientificaluz.org/index.php/kasmera/article/view/30834infecciones, antibióticos, gram negativos, antibiogramas |
| spellingShingle | Lorena Abadía-Patiño Carlos Enrique Bravo-Rodriguez José Luís Prin Saúl José Mosqueda-Paraqueimo Inhibition study of biofilms production of Pseudomonas aeruginosa strains in the presence of propolis Kasmera infecciones, antibióticos, gram negativos, antibiogramas |
| title | Inhibition study of biofilms production of Pseudomonas aeruginosa strains in the presence of propolis |
| title_full | Inhibition study of biofilms production of Pseudomonas aeruginosa strains in the presence of propolis |
| title_fullStr | Inhibition study of biofilms production of Pseudomonas aeruginosa strains in the presence of propolis |
| title_full_unstemmed | Inhibition study of biofilms production of Pseudomonas aeruginosa strains in the presence of propolis |
| title_short | Inhibition study of biofilms production of Pseudomonas aeruginosa strains in the presence of propolis |
| title_sort | inhibition study of biofilms production of pseudomonas aeruginosa strains in the presence of propolis |
| topic | infecciones, antibióticos, gram negativos, antibiogramas |
| url | http://produccioncientificaluz.org/index.php/kasmera/article/view/30834 |
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