Genome-Wide Characterization and Sequence Polymorphism Analyses of <i>Glycine max</i> Fibrillin (<i>FBN</i>) Revealed Its Role in Response to Drought Condition
The fibrillin (<i>FBN</i>) gene family is widely distributed in all photosynthetic organisms. Members of this gene family are involved in plant growth and development and their response to various biotic and abiotic stress factors. In this study, 16 members of <i>FBN</i> were...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/2073-4425/14/6/1188 |
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| author | Muhammad Zeshan Zafer Muhammad Hammad Nadeem Tahir Zulqurnain Khan Muhammad Sajjad Xiangkuo Gao Muhammad Amir Bakhtavar Ummara Waheed Maria Siddique Zhide Geng Shoaib Ur Rehman |
| author_facet | Muhammad Zeshan Zafer Muhammad Hammad Nadeem Tahir Zulqurnain Khan Muhammad Sajjad Xiangkuo Gao Muhammad Amir Bakhtavar Ummara Waheed Maria Siddique Zhide Geng Shoaib Ur Rehman |
| author_sort | Muhammad Zeshan Zafer |
| collection | DOAJ |
| description | The fibrillin (<i>FBN</i>) gene family is widely distributed in all photosynthetic organisms. Members of this gene family are involved in plant growth and development and their response to various biotic and abiotic stress factors. In this study, 16 members of <i>FBN</i> were identified in <i>Glycine max</i> and characterized by using different bioinformatics tools. Phylogenetic analysis classified <i>FBN</i> genes into seven groups. The presence of stress-related <i>cis</i>-elements in the upstream region of <i>GmFBN</i> highlighted their role in tolerance against abiotic stresses. To further decipher the function, physiochemical properties, conserved motifs, chromosomal localization, subcellular localization, and <i>cis</i>-acting regulatory elements were also analyzed. Gene expression analysis based on FPKM values revealed that <i>GmFBNs</i> greatly enhanced soybean drought tolerance and controlled the expression of several genes involved in drought response, except for <i>GmFBN-4</i>, <i>GmFBN-5</i>, <i>GmFBN-6</i>, <i>GmFBN-7</i> and <i>GmFBN-9</i>. For high throughput genotyping, an SNP-based CAPS marker was also developed for the <i>GmFBN-15</i> gene. The CAPS marker differentiated soybean genotypes based on the presence of either the <i>GmFBN-15-G</i> or <i>GmFBN-15-A</i> alleles in the CDS region. Association analysis showed that <i>G. max</i> accessions containing the <i>GmFBN-15-A</i> allele at the respective locus showed higher thousand seed weight compared to accessions containing the <i>GmFBN-15-G</i> allele. This research has provided the basic information to further decipher the function of <i>FBN</i> in soybean. |
| first_indexed | 2024-03-11T02:25:12Z |
| format | Article |
| id | doaj.art-39dbd30fb63e4acf82a46caf2b2a78eb |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T02:25:12Z |
| publishDate | 2023-05-01 |
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| series | Genes |
| spelling | doaj.art-39dbd30fb63e4acf82a46caf2b2a78eb2023-11-18T10:34:01ZengMDPI AGGenes2073-44252023-05-01146118810.3390/genes14061188Genome-Wide Characterization and Sequence Polymorphism Analyses of <i>Glycine max</i> Fibrillin (<i>FBN</i>) Revealed Its Role in Response to Drought ConditionMuhammad Zeshan Zafer0Muhammad Hammad Nadeem Tahir1Zulqurnain Khan2Muhammad Sajjad3Xiangkuo Gao4Muhammad Amir Bakhtavar5Ummara Waheed6Maria Siddique7Zhide Geng8Shoaib Ur Rehman9SINO-PAK Joint Research Laboratory, Institute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef, University of Agriculture, Multan 66000, PakistanInstitute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef, University of Agriculture, Multan 66000, PakistanSINO-PAK Joint Research Laboratory, Institute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef, University of Agriculture, Multan 66000, PakistanDepartment of Biosciences, COMSATS University, Islamabad (CUI), Park Road, Islamabad 45550, PakistanInstitute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650204, ChinaInstitute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef, University of Agriculture, Multan 66000, PakistanInstitute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef, University of Agriculture, Multan 66000, PakistanDepartment of Environmental Sciences, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, PakistanInstitute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650204, ChinaSINO-PAK Joint Research Laboratory, Institute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef, University of Agriculture, Multan 66000, PakistanThe fibrillin (<i>FBN</i>) gene family is widely distributed in all photosynthetic organisms. Members of this gene family are involved in plant growth and development and their response to various biotic and abiotic stress factors. In this study, 16 members of <i>FBN</i> were identified in <i>Glycine max</i> and characterized by using different bioinformatics tools. Phylogenetic analysis classified <i>FBN</i> genes into seven groups. The presence of stress-related <i>cis</i>-elements in the upstream region of <i>GmFBN</i> highlighted their role in tolerance against abiotic stresses. To further decipher the function, physiochemical properties, conserved motifs, chromosomal localization, subcellular localization, and <i>cis</i>-acting regulatory elements were also analyzed. Gene expression analysis based on FPKM values revealed that <i>GmFBNs</i> greatly enhanced soybean drought tolerance and controlled the expression of several genes involved in drought response, except for <i>GmFBN-4</i>, <i>GmFBN-5</i>, <i>GmFBN-6</i>, <i>GmFBN-7</i> and <i>GmFBN-9</i>. For high throughput genotyping, an SNP-based CAPS marker was also developed for the <i>GmFBN-15</i> gene. The CAPS marker differentiated soybean genotypes based on the presence of either the <i>GmFBN-15-G</i> or <i>GmFBN-15-A</i> alleles in the CDS region. Association analysis showed that <i>G. max</i> accessions containing the <i>GmFBN-15-A</i> allele at the respective locus showed higher thousand seed weight compared to accessions containing the <i>GmFBN-15-G</i> allele. This research has provided the basic information to further decipher the function of <i>FBN</i> in soybean.https://www.mdpi.com/2073-4425/14/6/1188<i>Glycine max</i>fibrillin (<i>FBN</i>)genome-wide identificationmarker-assisted breedingCAPSdrought |
| spellingShingle | Muhammad Zeshan Zafer Muhammad Hammad Nadeem Tahir Zulqurnain Khan Muhammad Sajjad Xiangkuo Gao Muhammad Amir Bakhtavar Ummara Waheed Maria Siddique Zhide Geng Shoaib Ur Rehman Genome-Wide Characterization and Sequence Polymorphism Analyses of <i>Glycine max</i> Fibrillin (<i>FBN</i>) Revealed Its Role in Response to Drought Condition Genes <i>Glycine max</i> fibrillin (<i>FBN</i>) genome-wide identification marker-assisted breeding CAPS drought |
| title | Genome-Wide Characterization and Sequence Polymorphism Analyses of <i>Glycine max</i> Fibrillin (<i>FBN</i>) Revealed Its Role in Response to Drought Condition |
| title_full | Genome-Wide Characterization and Sequence Polymorphism Analyses of <i>Glycine max</i> Fibrillin (<i>FBN</i>) Revealed Its Role in Response to Drought Condition |
| title_fullStr | Genome-Wide Characterization and Sequence Polymorphism Analyses of <i>Glycine max</i> Fibrillin (<i>FBN</i>) Revealed Its Role in Response to Drought Condition |
| title_full_unstemmed | Genome-Wide Characterization and Sequence Polymorphism Analyses of <i>Glycine max</i> Fibrillin (<i>FBN</i>) Revealed Its Role in Response to Drought Condition |
| title_short | Genome-Wide Characterization and Sequence Polymorphism Analyses of <i>Glycine max</i> Fibrillin (<i>FBN</i>) Revealed Its Role in Response to Drought Condition |
| title_sort | genome wide characterization and sequence polymorphism analyses of i glycine max i fibrillin i fbn i revealed its role in response to drought condition |
| topic | <i>Glycine max</i> fibrillin (<i>FBN</i>) genome-wide identification marker-assisted breeding CAPS drought |
| url | https://www.mdpi.com/2073-4425/14/6/1188 |
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