Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activity
Microbial production of oleochemicals involves strategies of expressing thioesterase to narrow the substrate pool for the termination enzyme at the expense of one ATP. Here, the authors developed an alternative energy-efficient strategy to use of an acyl-ACP transacylase to produce medium chain oleo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-29218-3 |
| _version_ | 1811335547311882240 |
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| author | Qiang Yan William T. Cordell Michael A. Jindra Dylan K. Courtney Madeline K. Kuckuk Xuanqi Chen Brian F. Pfleger |
| author_facet | Qiang Yan William T. Cordell Michael A. Jindra Dylan K. Courtney Madeline K. Kuckuk Xuanqi Chen Brian F. Pfleger |
| author_sort | Qiang Yan |
| collection | DOAJ |
| description | Microbial production of oleochemicals involves strategies of expressing thioesterase to narrow the substrate pool for the termination enzyme at the expense of one ATP. Here, the authors developed an alternative energy-efficient strategy to use of an acyl-ACP transacylase to produce medium chain oleochemicals in E. coli. |
| first_indexed | 2024-04-13T17:25:46Z |
| format | Article |
| id | doaj.art-39dd51880fbc497ba33cb80b8f5b33b6 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-13T17:25:46Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-39dd51880fbc497ba33cb80b8f5b33b62022-12-22T02:37:47ZengNature PortfolioNature Communications2041-17232022-03-0113111010.1038/s41467-022-29218-3Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activityQiang Yan0William T. Cordell1Michael A. Jindra2Dylan K. Courtney3Madeline K. Kuckuk4Xuanqi Chen5Brian F. Pfleger6Department of Chemical and Biological Engineering, University of Wisconsin-MadisonDepartment of Chemical and Biological Engineering, University of Wisconsin-MadisonDepartment of Chemical and Biological Engineering, University of Wisconsin-MadisonDepartment of Chemical and Biological Engineering, University of Wisconsin-MadisonDepartment of Chemical and Biological Engineering, University of Wisconsin-MadisonDepartment of Chemical and Biological Engineering, University of Wisconsin-MadisonDepartment of Chemical and Biological Engineering, University of Wisconsin-MadisonMicrobial production of oleochemicals involves strategies of expressing thioesterase to narrow the substrate pool for the termination enzyme at the expense of one ATP. Here, the authors developed an alternative energy-efficient strategy to use of an acyl-ACP transacylase to produce medium chain oleochemicals in E. coli.https://doi.org/10.1038/s41467-022-29218-3 |
| spellingShingle | Qiang Yan William T. Cordell Michael A. Jindra Dylan K. Courtney Madeline K. Kuckuk Xuanqi Chen Brian F. Pfleger Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activity Nature Communications |
| title | Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activity |
| title_full | Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activity |
| title_fullStr | Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activity |
| title_full_unstemmed | Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activity |
| title_short | Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activity |
| title_sort | metabolic engineering strategies to produce medium chain oleochemicals via acyl acp coa transacylase activity |
| url | https://doi.org/10.1038/s41467-022-29218-3 |
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