Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into <it>Streptomyces ansochromogenes </it>lead to an improved nikkomycin production
<p>Abstract</p> <p>Background</p> <p>Nikkomycins are a group of peptidyl nucleoside antibiotics produced by <it>Streptomyces ansochromogenes</it>. They are competitive inhibitors of chitin synthase and show potent fungicidal, insecticidal, and acaricidal act...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-01-01
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| Series: | Microbial Cell Factories |
| Online Access: | http://www.microbialcellfactories.com/content/9/1/6 |
| _version_ | 1811322214458327040 |
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| author | Yang Haihua Li Lei Li Jine Liao Guojian Tian Yuqing Tan Huarong |
| author_facet | Yang Haihua Li Lei Li Jine Liao Guojian Tian Yuqing Tan Huarong |
| author_sort | Yang Haihua |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Nikkomycins are a group of peptidyl nucleoside antibiotics produced by <it>Streptomyces ansochromogenes</it>. They are competitive inhibitors of chitin synthase and show potent fungicidal, insecticidal, and acaricidal activities. Nikkomycin X and Z are the main components produced by <it>S. ansochromogenes</it>. Generation of a high-producing strain is crucial to scale up nikkomycins production for further clinical trials.</p> <p>Results</p> <p>To increase the yields of nikkomycins, an additional copy of nikkomycin biosynthetic gene cluster (35 kb) was introduced into nikkomycin producing strain, <it>S. ansochromogenes </it>7100. The gene cluster was first reassembled into an integrative plasmid by Red/ET technology combining with classic cloning methods and then the resulting plasmid(pNIK)was introduced into <it>S. ansochromogenes </it>by conjugal transfer. Introduction of pNIK led to enhanced production of nikkomycins (880 mg L<sup>-1</sup>, 4 -fold nikkomycin X and 210 mg L<sup>-1</sup>, 1.8-fold nikkomycin Z) in the resulting exconjugants comparing with the parent strain (220 mg L<sup>-1 </sup>nikkomycin X and 120 mg L<sup>-1 </sup>nikkomycin Z). The exconjugants are genetically stable in the absence of antibiotic resistance selection pressure.</p> <p>Conclusion</p> <p>A high nikkomycins producing strain (1100 mg L<sup>-1 </sup>nikkomycins) was obtained by introduction of an extra nikkomycin biosynthetic gene cluster into the genome of <it>S. ansochromogenes</it>. The strategies presented here could be applicable to other bacteria to improve the yields of secondary metabolites.</p> |
| first_indexed | 2024-04-13T13:31:16Z |
| format | Article |
| id | doaj.art-39e0138c8b614eb3b4ad8faccfe86645 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-13T13:31:16Z |
| publishDate | 2010-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-39e0138c8b614eb3b4ad8faccfe866452022-12-22T02:44:56ZengBMCMicrobial Cell Factories1475-28592010-01-0191610.1186/1475-2859-9-6Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into <it>Streptomyces ansochromogenes </it>lead to an improved nikkomycin productionYang HaihuaLi LeiLi JineLiao GuojianTian YuqingTan Huarong<p>Abstract</p> <p>Background</p> <p>Nikkomycins are a group of peptidyl nucleoside antibiotics produced by <it>Streptomyces ansochromogenes</it>. They are competitive inhibitors of chitin synthase and show potent fungicidal, insecticidal, and acaricidal activities. Nikkomycin X and Z are the main components produced by <it>S. ansochromogenes</it>. Generation of a high-producing strain is crucial to scale up nikkomycins production for further clinical trials.</p> <p>Results</p> <p>To increase the yields of nikkomycins, an additional copy of nikkomycin biosynthetic gene cluster (35 kb) was introduced into nikkomycin producing strain, <it>S. ansochromogenes </it>7100. The gene cluster was first reassembled into an integrative plasmid by Red/ET technology combining with classic cloning methods and then the resulting plasmid(pNIK)was introduced into <it>S. ansochromogenes </it>by conjugal transfer. Introduction of pNIK led to enhanced production of nikkomycins (880 mg L<sup>-1</sup>, 4 -fold nikkomycin X and 210 mg L<sup>-1</sup>, 1.8-fold nikkomycin Z) in the resulting exconjugants comparing with the parent strain (220 mg L<sup>-1 </sup>nikkomycin X and 120 mg L<sup>-1 </sup>nikkomycin Z). The exconjugants are genetically stable in the absence of antibiotic resistance selection pressure.</p> <p>Conclusion</p> <p>A high nikkomycins producing strain (1100 mg L<sup>-1 </sup>nikkomycins) was obtained by introduction of an extra nikkomycin biosynthetic gene cluster into the genome of <it>S. ansochromogenes</it>. The strategies presented here could be applicable to other bacteria to improve the yields of secondary metabolites.</p>http://www.microbialcellfactories.com/content/9/1/6 |
| spellingShingle | Yang Haihua Li Lei Li Jine Liao Guojian Tian Yuqing Tan Huarong Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into <it>Streptomyces ansochromogenes </it>lead to an improved nikkomycin production Microbial Cell Factories |
| title | Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into <it>Streptomyces ansochromogenes </it>lead to an improved nikkomycin production |
| title_full | Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into <it>Streptomyces ansochromogenes </it>lead to an improved nikkomycin production |
| title_fullStr | Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into <it>Streptomyces ansochromogenes </it>lead to an improved nikkomycin production |
| title_full_unstemmed | Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into <it>Streptomyces ansochromogenes </it>lead to an improved nikkomycin production |
| title_short | Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into <it>Streptomyces ansochromogenes </it>lead to an improved nikkomycin production |
| title_sort | cloning reassembling and integration of the entire nikkomycin biosynthetic gene cluster into it streptomyces ansochromogenes it lead to an improved nikkomycin production |
| url | http://www.microbialcellfactories.com/content/9/1/6 |
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