Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections
Background: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicem...
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| Format: | Article |
| Language: | English |
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Veterinary World
2015-06-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.8/June-2015/17.pdf |
| _version_ | 1819137562263945216 |
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| author | Eric Lim Teik Chung Faez Firdaus Jesse Abdullah Lawan Adamu Ali Dhiaa Marza Hayder Hamzah Ibrahim Mohd Zamri-Saad Abdul Wahid Haron Abdul Aziz Saharee Mohd Azmi Mohd Lila Abdul Rahman Omar Md Zuki Abu Bakar Mohd Jefri Norsidin |
| author_facet | Eric Lim Teik Chung Faez Firdaus Jesse Abdullah Lawan Adamu Ali Dhiaa Marza Hayder Hamzah Ibrahim Mohd Zamri-Saad Abdul Wahid Haron Abdul Aziz Saharee Mohd Azmi Mohd Lila Abdul Rahman Omar Md Zuki Abu Bakar Mohd Jefri Norsidin |
| author_sort | Eric Lim Teik Chung |
| collection | DOAJ |
| description | Background: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicemic disease with high morbidity and mortality in cattle and more susceptible in buffaloes. Therefore, the aim of this study was to investigate the clinical signs, blood parameters, post mortem and histopathology changes caused by P. multocida Type B:2 infections initiated through the oral and subcutaneous routes.
Methods: Nine buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 ml of phosphate buffer saline; Groups 2 and 3 were inoculated with 10 ml of 1012 colony forming unit of P. multocida Type B:2 subcutaneously and orally respectively.
Results: There was a significant difference (p<0.05) in temperature between the subcutaneous and the control group. The results revealed significant differences (p<0.05) in erythrocytes, hemoglobin, packed cell volume, leukocytes, monocytes, and A: G ratio between the subcutaneous and the control group. Furthermore, there were significant differences (p<0.05) in leukocytes, band neutrophils, segmented neutrophils, lymphocytes, eosinophils, basophils, thrombocytes, plasma protein, icterus index, gamma glutamyl tranferase and A: G ratio between the oral and the control group. The post mortem lesions of the subcutaneous group buffaloes showed generalized hyperemia, congestion and hemorrhage of the immune organs, gastrointestinal tract organs and vital organs. The oral group buffaloes showed mild lesions in the lung and liver. Histologically, there were significant differences (p<0.05) in hemorrhage and congestion; necrosis and degeneration; inflammatory cells infiltration; and edema in between the groups.
Conclusion: This study was a proof that oral route infection of P. multocida Type B:2 can be used to stimulate host cell responses where oral vaccine through feed can be developed in the near future. |
| first_indexed | 2024-12-22T10:52:51Z |
| format | Article |
| id | doaj.art-39e08a4a0e6a40c599f6084b3c8a4320 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-22T10:52:51Z |
| publishDate | 2015-06-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-39e08a4a0e6a40c599f6084b3c8a43202022-12-21T18:28:42ZengVeterinary WorldVeterinary World0972-89882231-09162015-06-018678379210.14202/vetworld.2015.783-792Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infectionsEric Lim Teik Chung0Faez Firdaus Jesse Abdullah1Lawan Adamu2Ali Dhiaa Marza3Hayder Hamzah Ibrahim4Mohd Zamri-Saad5Abdul Wahid Haron6Abdul Aziz Saharee7Mohd Azmi Mohd Lila8Abdul Rahman Omar9Md Zuki Abu Bakar 10Mohd Jefri Norsidin11Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; eric88lim@gmail.comDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Ruminant Disease, Research Centre for Ruminant Disease, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; jesseariasamy@gmail.comDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Maiduguri, PMB1069, Borno State, Nigeria; drlawan3758@yahoo.comDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Veterinary Internal Medicine, Faculty of Veterinary Medicine, Al-Qasim Green University, Iraq; ali.alhashimi471969@yahoo.comDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Veterinary Medicine, Technical Institute Babil, Al Furat Alawast Technical University, Iraq; altee.hayder@yahoo.comDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; mzamri@upm.edu.myDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Ruminant Disease, Research Centre for Ruminant Disease, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; wahidh@upm.edu.myDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; abdaziz@upm.edu.myDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; azmi@upm.edu.myDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; aro@upm.edu.myDepartment of Preclinical, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; zuki@upm.edu.myDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; jaretguli@gmail.comBackground: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicemic disease with high morbidity and mortality in cattle and more susceptible in buffaloes. Therefore, the aim of this study was to investigate the clinical signs, blood parameters, post mortem and histopathology changes caused by P. multocida Type B:2 infections initiated through the oral and subcutaneous routes. Methods: Nine buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 ml of phosphate buffer saline; Groups 2 and 3 were inoculated with 10 ml of 1012 colony forming unit of P. multocida Type B:2 subcutaneously and orally respectively. Results: There was a significant difference (p<0.05) in temperature between the subcutaneous and the control group. The results revealed significant differences (p<0.05) in erythrocytes, hemoglobin, packed cell volume, leukocytes, monocytes, and A: G ratio between the subcutaneous and the control group. Furthermore, there were significant differences (p<0.05) in leukocytes, band neutrophils, segmented neutrophils, lymphocytes, eosinophils, basophils, thrombocytes, plasma protein, icterus index, gamma glutamyl tranferase and A: G ratio between the oral and the control group. The post mortem lesions of the subcutaneous group buffaloes showed generalized hyperemia, congestion and hemorrhage of the immune organs, gastrointestinal tract organs and vital organs. The oral group buffaloes showed mild lesions in the lung and liver. Histologically, there were significant differences (p<0.05) in hemorrhage and congestion; necrosis and degeneration; inflammatory cells infiltration; and edema in between the groups. Conclusion: This study was a proof that oral route infection of P. multocida Type B:2 can be used to stimulate host cell responses where oral vaccine through feed can be developed in the near future.http://www.veterinaryworld.org/Vol.8/June-2015/17.pdfbuffalo heifersclinico-pathologyhematology and biochemistry responsesoral routePasteurella multocida Type B:2subcutaneous route |
| spellingShingle | Eric Lim Teik Chung Faez Firdaus Jesse Abdullah Lawan Adamu Ali Dhiaa Marza Hayder Hamzah Ibrahim Mohd Zamri-Saad Abdul Wahid Haron Abdul Aziz Saharee Mohd Azmi Mohd Lila Abdul Rahman Omar Md Zuki Abu Bakar Mohd Jefri Norsidin Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections Veterinary World buffalo heifers clinico-pathology hematology and biochemistry responses oral route Pasteurella multocida Type B:2 subcutaneous route |
| title | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections |
| title_full | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections |
| title_fullStr | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections |
| title_full_unstemmed | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections |
| title_short | Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections |
| title_sort | clinico pathology hematology and biochemistry responses toward pasteurella multocida type b 2 via oral and subcutaneous route of infections |
| topic | buffalo heifers clinico-pathology hematology and biochemistry responses oral route Pasteurella multocida Type B:2 subcutaneous route |
| url | http://www.veterinaryworld.org/Vol.8/June-2015/17.pdf |
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