Analytical validation of anti-toxoplasma IgG immunoassays
There are often discrepancies when using different methods to measure anti-Toxoplasma gondii IgG levels in patient samples. The diagnostic performance of a chemiluminescent immunoassay (CLIA) and an enzyme-linked fluorescent assay (ELFA) used as confirmatory tests for samples identified as positive...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-12-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600012 |
| _version_ | 1819265033440329728 |
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| author | Guenael Freire de Souza Darlene Carvalho William Pedrosa Jacqueline Franck Renaud Piarroux |
| author_facet | Guenael Freire de Souza Darlene Carvalho William Pedrosa Jacqueline Franck Renaud Piarroux |
| author_sort | Guenael Freire de Souza |
| collection | DOAJ |
| description | There are often discrepancies when using different methods to measure anti-Toxoplasma gondii IgG levels in patient samples. The diagnostic performance of a chemiluminescent immunoassay (CLIA) and an enzyme-linked fluorescent assay (ELFA) used as confirmatory tests for samples identified as positive or equivocal by an electrochemiluminescent immunoassay (ECLIA) were examined. Cut-off values were those stated by the manufacturer, and Western blot was used to confirm the results of all methods. All samples identified as positive by ECLIA (n = 93) were confirmed as positive by Western blot, as were 14 of the 28 samples identified as equivocal. When these 121 samples were retested, the sensitivities of CLIA and ELFA were 64.4% and 73.8%, respectively. Both methods exhibited a specificity of 100%. This study confirms that the results obtained from the different immunoassays are not comparable, and neither CLIA nor ELFA should be used to confirm ECLIA results, which should instead be confirmed by methods such as Western blot or Sabin-Feldman dye test. |
| first_indexed | 2024-12-23T20:38:57Z |
| format | Article |
| id | doaj.art-39e0b2e328c641138ef58c9d21cb7381 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 1678-4391 |
| language | English |
| last_indexed | 2024-12-23T20:38:57Z |
| publishDate | 2012-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-39e0b2e328c641138ef58c9d21cb73812022-12-21T17:31:59ZengElsevierBrazilian Journal of Infectious Diseases1413-86701678-43912012-12-0116657457610.1590/S1413-86702012000600012Analytical validation of anti-toxoplasma IgG immunoassaysGuenael Freire de SouzaDarlene CarvalhoWilliam PedrosaJacqueline FranckRenaud PiarrouxThere are often discrepancies when using different methods to measure anti-Toxoplasma gondii IgG levels in patient samples. The diagnostic performance of a chemiluminescent immunoassay (CLIA) and an enzyme-linked fluorescent assay (ELFA) used as confirmatory tests for samples identified as positive or equivocal by an electrochemiluminescent immunoassay (ECLIA) were examined. Cut-off values were those stated by the manufacturer, and Western blot was used to confirm the results of all methods. All samples identified as positive by ECLIA (n = 93) were confirmed as positive by Western blot, as were 14 of the 28 samples identified as equivocal. When these 121 samples were retested, the sensitivities of CLIA and ELFA were 64.4% and 73.8%, respectively. Both methods exhibited a specificity of 100%. This study confirms that the results obtained from the different immunoassays are not comparable, and neither CLIA nor ELFA should be used to confirm ECLIA results, which should instead be confirmed by methods such as Western blot or Sabin-Feldman dye test.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600012ToxoplasmaImmunoassayECLIAELFA |
| spellingShingle | Guenael Freire de Souza Darlene Carvalho William Pedrosa Jacqueline Franck Renaud Piarroux Analytical validation of anti-toxoplasma IgG immunoassays Brazilian Journal of Infectious Diseases Toxoplasma Immunoassay ECLIA ELFA |
| title | Analytical validation of anti-toxoplasma IgG immunoassays |
| title_full | Analytical validation of anti-toxoplasma IgG immunoassays |
| title_fullStr | Analytical validation of anti-toxoplasma IgG immunoassays |
| title_full_unstemmed | Analytical validation of anti-toxoplasma IgG immunoassays |
| title_short | Analytical validation of anti-toxoplasma IgG immunoassays |
| title_sort | analytical validation of anti toxoplasma igg immunoassays |
| topic | Toxoplasma Immunoassay ECLIA ELFA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600012 |
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