The Characterization and Cytotoxic Evaluation of <i>Chondrosia reniformis</i> Collagen Isolated from Different Body Parts (Ectosome and Choanosome) Envisaging the Development of Biomaterials
<i>Chondrosia reniformis</i> is a collagen-rich marine sponge that is considered a sustainable and viable option for producing an alternative to mammalian-origin collagens. However, there is a lack of knowledge regarding the properties of collagen isolated from different sponge parts, na...
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MDPI AG
2024-01-01
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| Series: | Marine Drugs |
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| author | Miguel S. Rocha Catarina F. Marques Ana C. Carvalho Eva Martins Alexander Ereskovsky Rui L. Reis Tiago H. Silva |
| author_facet | Miguel S. Rocha Catarina F. Marques Ana C. Carvalho Eva Martins Alexander Ereskovsky Rui L. Reis Tiago H. Silva |
| author_sort | Miguel S. Rocha |
| collection | DOAJ |
| description | <i>Chondrosia reniformis</i> is a collagen-rich marine sponge that is considered a sustainable and viable option for producing an alternative to mammalian-origin collagens. However, there is a lack of knowledge regarding the properties of collagen isolated from different sponge parts, namely the outer region, or cortex, (ectosome) and the inner region (choanosome), and how it affects the development of biomaterials. In this study, a brief histological analysis focusing on <i>C. reniformis</i> collagen spatial distribution and a comprehensive comparative analysis between collagen isolated from ectosome and choanosome are presented. The isolated collagen characterization was based on isolation yield, Fourier-transformed infrared spectroscopy (FTIR), circular dichroism (CD), SDS-PAGE, dot blot, and amino acid composition, as well as their cytocompatibility envisaging the development of future biomedical applications. An isolation yield of approximately 20% was similar for both sponge parts, as well as the FTIR, CD, and SDS-PAGE profiles, which demonstrated that both isolated collagens presented a high purity degree and preserved their triple helix and fibrillar conformation. Ectosome collagen had a higher OHpro content and possessed collagen type I and IV, while the choanosome was predominately constituted by collagen type IV. In vitro cytotoxicity assays using the L929 fibroblast cell line displayed a significant cytotoxic effect of choanosome collagen at 2 mg/mL, while ectosome collagen enhanced cell metabolism and proliferation, thus indicating the latter as being more suitable for the development of biomaterials. This research represents a unique comparative study of <i>C. reniformis</i> body parts, serving as a support for further establishing this marine sponge as a promising alternative collagen source for the future development of biomedical applications. |
| first_indexed | 2024-03-07T22:23:38Z |
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| language | English |
| last_indexed | 2024-03-07T22:23:38Z |
| publishDate | 2024-01-01 |
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| series | Marine Drugs |
| spelling | doaj.art-39e25bcc0ca6425a86b1d1abe5050d142024-02-23T15:25:12ZengMDPI AGMarine Drugs1660-33972024-01-012225510.3390/md22020055The Characterization and Cytotoxic Evaluation of <i>Chondrosia reniformis</i> Collagen Isolated from Different Body Parts (Ectosome and Choanosome) Envisaging the Development of BiomaterialsMiguel S. Rocha0Catarina F. Marques1Ana C. Carvalho2Eva Martins3Alexander Ereskovsky4Rui L. Reis5Tiago H. Silva63B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimaraes, Portugal3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimaraes, Portugal3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimaraes, Portugal3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimaraes, PortugalInstitut Méditerranéen de Biodiversité et d’Ecologie Marine et Continentale (IMBE), Aix Marseille University, Avignon University, Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD), 13007 Marseille, France3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimaraes, Portugal3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimaraes, Portugal<i>Chondrosia reniformis</i> is a collagen-rich marine sponge that is considered a sustainable and viable option for producing an alternative to mammalian-origin collagens. However, there is a lack of knowledge regarding the properties of collagen isolated from different sponge parts, namely the outer region, or cortex, (ectosome) and the inner region (choanosome), and how it affects the development of biomaterials. In this study, a brief histological analysis focusing on <i>C. reniformis</i> collagen spatial distribution and a comprehensive comparative analysis between collagen isolated from ectosome and choanosome are presented. The isolated collagen characterization was based on isolation yield, Fourier-transformed infrared spectroscopy (FTIR), circular dichroism (CD), SDS-PAGE, dot blot, and amino acid composition, as well as their cytocompatibility envisaging the development of future biomedical applications. An isolation yield of approximately 20% was similar for both sponge parts, as well as the FTIR, CD, and SDS-PAGE profiles, which demonstrated that both isolated collagens presented a high purity degree and preserved their triple helix and fibrillar conformation. Ectosome collagen had a higher OHpro content and possessed collagen type I and IV, while the choanosome was predominately constituted by collagen type IV. In vitro cytotoxicity assays using the L929 fibroblast cell line displayed a significant cytotoxic effect of choanosome collagen at 2 mg/mL, while ectosome collagen enhanced cell metabolism and proliferation, thus indicating the latter as being more suitable for the development of biomaterials. This research represents a unique comparative study of <i>C. reniformis</i> body parts, serving as a support for further establishing this marine sponge as a promising alternative collagen source for the future development of biomedical applications.https://www.mdpi.com/1660-3397/22/2/55collagen<i>C. reniformis</i>marine spongesectosomechoanosome |
| spellingShingle | Miguel S. Rocha Catarina F. Marques Ana C. Carvalho Eva Martins Alexander Ereskovsky Rui L. Reis Tiago H. Silva The Characterization and Cytotoxic Evaluation of <i>Chondrosia reniformis</i> Collagen Isolated from Different Body Parts (Ectosome and Choanosome) Envisaging the Development of Biomaterials Marine Drugs collagen <i>C. reniformis</i> marine sponges ectosome choanosome |
| title | The Characterization and Cytotoxic Evaluation of <i>Chondrosia reniformis</i> Collagen Isolated from Different Body Parts (Ectosome and Choanosome) Envisaging the Development of Biomaterials |
| title_full | The Characterization and Cytotoxic Evaluation of <i>Chondrosia reniformis</i> Collagen Isolated from Different Body Parts (Ectosome and Choanosome) Envisaging the Development of Biomaterials |
| title_fullStr | The Characterization and Cytotoxic Evaluation of <i>Chondrosia reniformis</i> Collagen Isolated from Different Body Parts (Ectosome and Choanosome) Envisaging the Development of Biomaterials |
| title_full_unstemmed | The Characterization and Cytotoxic Evaluation of <i>Chondrosia reniformis</i> Collagen Isolated from Different Body Parts (Ectosome and Choanosome) Envisaging the Development of Biomaterials |
| title_short | The Characterization and Cytotoxic Evaluation of <i>Chondrosia reniformis</i> Collagen Isolated from Different Body Parts (Ectosome and Choanosome) Envisaging the Development of Biomaterials |
| title_sort | characterization and cytotoxic evaluation of i chondrosia reniformis i collagen isolated from different body parts ectosome and choanosome envisaging the development of biomaterials |
| topic | collagen <i>C. reniformis</i> marine sponges ectosome choanosome |
| url | https://www.mdpi.com/1660-3397/22/2/55 |
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