Neutrophils Mediate Pulmonary Artery Thrombosis In Situ
Pulmonary embolism is a life-threatening condition, which can result in respiratory insufficiency and death. Blood clots occluding branches of the pulmonary artery (PA) are traditionally considered to originate from thrombi in deep veins (usually in legs). However, growing evidence suggests that occ...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5829 |
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| author | Olga Porembskaya Vsevolod Zinserling Vladimir Tomson Yana Toropova Eleonora A. Starikova Vitaliy V. Maslei Nika I. Bulavinova Olga V. Kirik Marina A. Syrtsova Leonid Laberko Maxim I. Galchenko Vyacheslav Kravchuk Sergey Saiganov Alexander Brill |
| author_facet | Olga Porembskaya Vsevolod Zinserling Vladimir Tomson Yana Toropova Eleonora A. Starikova Vitaliy V. Maslei Nika I. Bulavinova Olga V. Kirik Marina A. Syrtsova Leonid Laberko Maxim I. Galchenko Vyacheslav Kravchuk Sergey Saiganov Alexander Brill |
| author_sort | Olga Porembskaya |
| collection | DOAJ |
| description | Pulmonary embolism is a life-threatening condition, which can result in respiratory insufficiency and death. Blood clots occluding branches of the pulmonary artery (PA) are traditionally considered to originate from thrombi in deep veins (usually in legs). However, growing evidence suggests that occlusion of the vessels in the lungs can develop without preceding deep vein thrombosis (DVT). In this work, we used an inferior vena cava (IVC) complete ligation model of DVT in Wistar rats to explore the possibility and mechanisms of PA thrombosis under the conditions where all routes of thrombotic mass migration from peripheral veins are blocked. We demonstrate that rats both with normal and reduced neutrophil counts developed thrombi in the IVC, although, neutropenia caused a substantial decrease in thrombus size and a shift from fresh fibrin toward mature fibrin and connective tissue inside the thrombus. Massive fibrin deposition was found in the PA branches in the majority of DVT rats with normal neutrophil counts, but in none of the neutropenic animals. Neutrophil ablation also abolished macroscopic signs of lung damage. Altogether, the results demonstrate that thrombi in the lung vasculature can form in situ by mechanisms that require local neutrophil recruitment taking place in the DVT setting. |
| first_indexed | 2024-03-10T03:42:18Z |
| format | Article |
| id | doaj.art-39e463e8e9f64339aee835a09c6c1e45 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:42:18Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-39e463e8e9f64339aee835a09c6c1e452023-11-23T11:29:09ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310582910.3390/ijms23105829Neutrophils Mediate Pulmonary Artery Thrombosis In SituOlga Porembskaya0Vsevolod Zinserling1Vladimir Tomson2Yana Toropova3Eleonora A. Starikova4Vitaliy V. Maslei5Nika I. Bulavinova6Olga V. Kirik7Marina A. Syrtsova8Leonid Laberko9Maxim I. Galchenko10Vyacheslav Kravchuk11Sergey Saiganov12Alexander Brill13Cardio-Vascular Department, Mechnikov North-Western State Medical University, 191015 Saint Petersburg, RussiaInstitute of Experimental Medicine, Almazov National Medical Research Center, 197341 Saint Petersburg, RussiaScientific and Research Center, Pavlov University, 197022 Saint Petersburg, RussiaInstitute of Experimental Medicine, Almazov National Medical Research Center, 197341 Saint Petersburg, RussiaDepartment of Immunology, Institute of Experimental Medicine, 197376 Saint Petersburg, RussiaInstitute of Experimental Medicine, Almazov National Medical Research Center, 197341 Saint Petersburg, RussiaInstitute of Experimental Medicine, Almazov National Medical Research Center, 197341 Saint Petersburg, RussiaDepartment of Immunology, Institute of Experimental Medicine, 197376 Saint Petersburg, RussiaNovartis, 194362 Saint Petersburg, RussiaDepartment of General Surgery and Radiology, Pirogov Russian National Research Medical University, 117997 Moscow, RussiaDepartment of Electrical Engineering and Electrical Equipment, State Agrarian University, 196601 Saint Petersburg, RussiaCardio-Vascular Department, Mechnikov North-Western State Medical University, 191015 Saint Petersburg, RussiaCardio-Vascular Department, Mechnikov North-Western State Medical University, 191015 Saint Petersburg, RussiaInstitute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UKPulmonary embolism is a life-threatening condition, which can result in respiratory insufficiency and death. Blood clots occluding branches of the pulmonary artery (PA) are traditionally considered to originate from thrombi in deep veins (usually in legs). However, growing evidence suggests that occlusion of the vessels in the lungs can develop without preceding deep vein thrombosis (DVT). In this work, we used an inferior vena cava (IVC) complete ligation model of DVT in Wistar rats to explore the possibility and mechanisms of PA thrombosis under the conditions where all routes of thrombotic mass migration from peripheral veins are blocked. We demonstrate that rats both with normal and reduced neutrophil counts developed thrombi in the IVC, although, neutropenia caused a substantial decrease in thrombus size and a shift from fresh fibrin toward mature fibrin and connective tissue inside the thrombus. Massive fibrin deposition was found in the PA branches in the majority of DVT rats with normal neutrophil counts, but in none of the neutropenic animals. Neutrophil ablation also abolished macroscopic signs of lung damage. Altogether, the results demonstrate that thrombi in the lung vasculature can form in situ by mechanisms that require local neutrophil recruitment taking place in the DVT setting.https://www.mdpi.com/1422-0067/23/10/5829deep vein thrombosispulmonary embolismneutrophils |
| spellingShingle | Olga Porembskaya Vsevolod Zinserling Vladimir Tomson Yana Toropova Eleonora A. Starikova Vitaliy V. Maslei Nika I. Bulavinova Olga V. Kirik Marina A. Syrtsova Leonid Laberko Maxim I. Galchenko Vyacheslav Kravchuk Sergey Saiganov Alexander Brill Neutrophils Mediate Pulmonary Artery Thrombosis In Situ International Journal of Molecular Sciences deep vein thrombosis pulmonary embolism neutrophils |
| title | Neutrophils Mediate Pulmonary Artery Thrombosis In Situ |
| title_full | Neutrophils Mediate Pulmonary Artery Thrombosis In Situ |
| title_fullStr | Neutrophils Mediate Pulmonary Artery Thrombosis In Situ |
| title_full_unstemmed | Neutrophils Mediate Pulmonary Artery Thrombosis In Situ |
| title_short | Neutrophils Mediate Pulmonary Artery Thrombosis In Situ |
| title_sort | neutrophils mediate pulmonary artery thrombosis in situ |
| topic | deep vein thrombosis pulmonary embolism neutrophils |
| url | https://www.mdpi.com/1422-0067/23/10/5829 |
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