Cloning and Functional Identification of <i>SlPG49</i> in <i>Solanum lycopersicum</i>
The modification and degradation of pectin in cell walls are necessary for the fruit softening process, which involves a series of pectin-modifying enzymes. Polygalacturonases (PGs) are a major group of pectin-hydrolyzing enzymes, which participate in fruit maturation, organ shedding, pollen develop...
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MDPI AG
2021-12-01
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| Series: | Applied Sciences |
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| author | Weiqiang Li Liai Xu Rui Xia Ying Shen Zhujun Zhu Youjian Yu Yunxiang Zang |
| author_facet | Weiqiang Li Liai Xu Rui Xia Ying Shen Zhujun Zhu Youjian Yu Yunxiang Zang |
| author_sort | Weiqiang Li |
| collection | DOAJ |
| description | The modification and degradation of pectin in cell walls are necessary for the fruit softening process, which involves a series of pectin-modifying enzymes. Polygalacturonases (PGs) are a major group of pectin-hydrolyzing enzymes, which participate in fruit maturation, organ shedding, pollen development, and other processes by catalyzing the degradation of polygalacturonic acid. However, their function in plants has not yet been fully elucidated. In this paper, a full-length cDNA encoding SlPG49 was cloned from a tomato. Sequence alignment and phylogenetic analysis demonstrated that SlPG49 contains four typical conserved domains and belongs to clade E in PG classification. Quantitative real-time PCR analysis showed that <i>SlPG49</i> was highly expressed in fruits during the softening stage, indicating that <i>SlPG49</i> may be involved in fruit softening. Subcellular localization results revealed that SlPG49 was located in the cell membrane and the cell wall. In addition, an in vitro enzymatic activity assay confirmed that SlPG49 does have the ability to catalyze the hydrolysis of polygalacturonic acid. These results indicate that <i>SlPG49</i> is a newly discovered PG gene involved in tomato fruit softening, and provide an experimental basis for elucidating the biological functions of plant PGs during fruit softening. |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T04:57:03Z |
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| spelling | doaj.art-39e835f177814794877fcec074842ff92023-11-23T02:08:24ZengMDPI AGApplied Sciences2076-34172021-12-0111231145010.3390/app112311450Cloning and Functional Identification of <i>SlPG49</i> in <i>Solanum lycopersicum</i>Weiqiang Li0Liai Xu1Rui Xia2Ying Shen3Zhujun Zhu4Youjian Yu5Yunxiang Zang6Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaThe modification and degradation of pectin in cell walls are necessary for the fruit softening process, which involves a series of pectin-modifying enzymes. Polygalacturonases (PGs) are a major group of pectin-hydrolyzing enzymes, which participate in fruit maturation, organ shedding, pollen development, and other processes by catalyzing the degradation of polygalacturonic acid. However, their function in plants has not yet been fully elucidated. In this paper, a full-length cDNA encoding SlPG49 was cloned from a tomato. Sequence alignment and phylogenetic analysis demonstrated that SlPG49 contains four typical conserved domains and belongs to clade E in PG classification. Quantitative real-time PCR analysis showed that <i>SlPG49</i> was highly expressed in fruits during the softening stage, indicating that <i>SlPG49</i> may be involved in fruit softening. Subcellular localization results revealed that SlPG49 was located in the cell membrane and the cell wall. In addition, an in vitro enzymatic activity assay confirmed that SlPG49 does have the ability to catalyze the hydrolysis of polygalacturonic acid. These results indicate that <i>SlPG49</i> is a newly discovered PG gene involved in tomato fruit softening, and provide an experimental basis for elucidating the biological functions of plant PGs during fruit softening.https://www.mdpi.com/2076-3417/11/23/11450polygalacturonases (PG)<i>Solanum lycopersicum</i>SlPG49gene expressionenzymatic activity |
| spellingShingle | Weiqiang Li Liai Xu Rui Xia Ying Shen Zhujun Zhu Youjian Yu Yunxiang Zang Cloning and Functional Identification of <i>SlPG49</i> in <i>Solanum lycopersicum</i> Applied Sciences polygalacturonases (PG) <i>Solanum lycopersicum</i> SlPG49 gene expression enzymatic activity |
| title | Cloning and Functional Identification of <i>SlPG49</i> in <i>Solanum lycopersicum</i> |
| title_full | Cloning and Functional Identification of <i>SlPG49</i> in <i>Solanum lycopersicum</i> |
| title_fullStr | Cloning and Functional Identification of <i>SlPG49</i> in <i>Solanum lycopersicum</i> |
| title_full_unstemmed | Cloning and Functional Identification of <i>SlPG49</i> in <i>Solanum lycopersicum</i> |
| title_short | Cloning and Functional Identification of <i>SlPG49</i> in <i>Solanum lycopersicum</i> |
| title_sort | cloning and functional identification of i slpg49 i in i solanum lycopersicum i |
| topic | polygalacturonases (PG) <i>Solanum lycopersicum</i> SlPG49 gene expression enzymatic activity |
| url | https://www.mdpi.com/2076-3417/11/23/11450 |
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