Mulberry Leaf (<i>Morus alba</i> L.) Extracts and Its Chlorogenic Acid Isomer Component Improve Glucolipotoxicity-Induced Hepatic Lipid Accumulation via Downregulating miR-34a and Decreased Inflammation
Mulberry leaf (<i>Morus alba</i> L.) is used as a traditional medicine and potential health food to treat various metabolic diseases, such as hypertension, diabetes, and hyperlipidemia. However, we sought the mechanisms by which functional components of mulberry leaves mediate diabetic s...
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MDPI AG
2022-11-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/22/4808 |
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| author | Tsung-Yuan Yang Meng-Hsun Yu Yi-Liang Wu Ching-Chun Hong Chin-Shuh Chen Kuei-Chuan Chan Chau-Jong Wang |
| author_facet | Tsung-Yuan Yang Meng-Hsun Yu Yi-Liang Wu Ching-Chun Hong Chin-Shuh Chen Kuei-Chuan Chan Chau-Jong Wang |
| author_sort | Tsung-Yuan Yang |
| collection | DOAJ |
| description | Mulberry leaf (<i>Morus alba</i> L.) is used as a traditional medicine and potential health food to treat various metabolic diseases, such as hypertension, diabetes, and hyperlipidemia. However, we sought the mechanisms by which functional components of mulberry leaves mediate diabetic steatohepatitis. We applied an in vitro model of HepG2 cells induced by glucolipotoxicity and evaluated the effects of MLE and its major components nCGA, Crp, and CGA. The results showed that MLE and nCGA reduced liver fat accumulation by inhibiting SREBP-1/FASN, SREBP-2/HMG-CoAR, and activating PPARα/CPT-1. Additionally, MLE and nCGA decreased inflammatory responses associated with NF-κB, TNF-α, and IL-6 to alleviate steatohepatitis. Furthermore, we showed that MLE and nCGA exerted anti-glucolipotoxicity effects by downregulating miR-34a, thus activating SIRT1/AMPK signaling, and subsequently suppressing hepatic lipid accumulation. |
| first_indexed | 2024-03-09T18:05:50Z |
| format | Article |
| id | doaj.art-39e855313fc845578ddb693df677657d |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T18:05:50Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-39e855313fc845578ddb693df677657d2023-11-24T09:31:29ZengMDPI AGNutrients2072-66432022-11-011422480810.3390/nu14224808Mulberry Leaf (<i>Morus alba</i> L.) Extracts and Its Chlorogenic Acid Isomer Component Improve Glucolipotoxicity-Induced Hepatic Lipid Accumulation via Downregulating miR-34a and Decreased InflammationTsung-Yuan Yang0Meng-Hsun Yu1Yi-Liang Wu2Ching-Chun Hong3Chin-Shuh Chen4Kuei-Chuan Chan5Chau-Jong Wang6Department of Internal Medicine, Chung-Shan Medical University Hospital, No. 110, Section 1, Jianguo N. Road, Taichung 402, TaiwanDepartment of Health Industry Technology Management, Chung Shan Medical University, No. 110, Section 1, Jianguo N. Road, Taichung 402, TaiwanDivision of Cardiovascular Surgery, Surgical Department, Chung Shan Medical University Hospital, No. 110, Section 1, Jianguo N. Road, Taichung 402, TaiwanDepartment of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, TaiwanDepartment of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, TaiwanDepartment of Internal Medicine, Chung-Shan Medical University Hospital, No. 110, Section 1, Jianguo N. Road, Taichung 402, TaiwanDepartment of Health Industry Technology Management, Chung Shan Medical University, No. 110, Section 1, Jianguo N. Road, Taichung 402, TaiwanMulberry leaf (<i>Morus alba</i> L.) is used as a traditional medicine and potential health food to treat various metabolic diseases, such as hypertension, diabetes, and hyperlipidemia. However, we sought the mechanisms by which functional components of mulberry leaves mediate diabetic steatohepatitis. We applied an in vitro model of HepG2 cells induced by glucolipotoxicity and evaluated the effects of MLE and its major components nCGA, Crp, and CGA. The results showed that MLE and nCGA reduced liver fat accumulation by inhibiting SREBP-1/FASN, SREBP-2/HMG-CoAR, and activating PPARα/CPT-1. Additionally, MLE and nCGA decreased inflammatory responses associated with NF-κB, TNF-α, and IL-6 to alleviate steatohepatitis. Furthermore, we showed that MLE and nCGA exerted anti-glucolipotoxicity effects by downregulating miR-34a, thus activating SIRT1/AMPK signaling, and subsequently suppressing hepatic lipid accumulation.https://www.mdpi.com/2072-6643/14/22/4808mulberry leaf extractneochlorogenic acidnonalcoholic fatty liver diseasehepatic lipid accumulation and inflammationmiR-34a |
| spellingShingle | Tsung-Yuan Yang Meng-Hsun Yu Yi-Liang Wu Ching-Chun Hong Chin-Shuh Chen Kuei-Chuan Chan Chau-Jong Wang Mulberry Leaf (<i>Morus alba</i> L.) Extracts and Its Chlorogenic Acid Isomer Component Improve Glucolipotoxicity-Induced Hepatic Lipid Accumulation via Downregulating miR-34a and Decreased Inflammation Nutrients mulberry leaf extract neochlorogenic acid nonalcoholic fatty liver disease hepatic lipid accumulation and inflammation miR-34a |
| title | Mulberry Leaf (<i>Morus alba</i> L.) Extracts and Its Chlorogenic Acid Isomer Component Improve Glucolipotoxicity-Induced Hepatic Lipid Accumulation via Downregulating miR-34a and Decreased Inflammation |
| title_full | Mulberry Leaf (<i>Morus alba</i> L.) Extracts and Its Chlorogenic Acid Isomer Component Improve Glucolipotoxicity-Induced Hepatic Lipid Accumulation via Downregulating miR-34a and Decreased Inflammation |
| title_fullStr | Mulberry Leaf (<i>Morus alba</i> L.) Extracts and Its Chlorogenic Acid Isomer Component Improve Glucolipotoxicity-Induced Hepatic Lipid Accumulation via Downregulating miR-34a and Decreased Inflammation |
| title_full_unstemmed | Mulberry Leaf (<i>Morus alba</i> L.) Extracts and Its Chlorogenic Acid Isomer Component Improve Glucolipotoxicity-Induced Hepatic Lipid Accumulation via Downregulating miR-34a and Decreased Inflammation |
| title_short | Mulberry Leaf (<i>Morus alba</i> L.) Extracts and Its Chlorogenic Acid Isomer Component Improve Glucolipotoxicity-Induced Hepatic Lipid Accumulation via Downregulating miR-34a and Decreased Inflammation |
| title_sort | mulberry leaf i morus alba i l extracts and its chlorogenic acid isomer component improve glucolipotoxicity induced hepatic lipid accumulation via downregulating mir 34a and decreased inflammation |
| topic | mulberry leaf extract neochlorogenic acid nonalcoholic fatty liver disease hepatic lipid accumulation and inflammation miR-34a |
| url | https://www.mdpi.com/2072-6643/14/22/4808 |
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