Genomic Features and Molecular Function of a Novel Stress-Tolerant <i>Bacillus halotolerans</i> Strain Isolated from an Extreme Environment
Due to its topographical position and climatic conditions, the Qinghai–Tibet Plateau possesses abundant microorganism resources. The extremophilic strain KKD1 isolated from Hoh Xil possesses strong stress tolerance, enabling it to propagate under high salinity (13%) and alkalinity (pH 10.0) conditio...
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MDPI AG
2021-10-01
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| author | Xiaohui Wu Huijun Wu Ruoyi Wang Zhengqi Wang Yaming Zhang Qin Gu Ayaz Farzand Xue Yang Mikhail Semenov Rainer Borriss Yongli Xie Xuewen Gao |
| author_facet | Xiaohui Wu Huijun Wu Ruoyi Wang Zhengqi Wang Yaming Zhang Qin Gu Ayaz Farzand Xue Yang Mikhail Semenov Rainer Borriss Yongli Xie Xuewen Gao |
| author_sort | Xiaohui Wu |
| collection | DOAJ |
| description | Due to its topographical position and climatic conditions, the Qinghai–Tibet Plateau possesses abundant microorganism resources. The extremophilic strain KKD1 isolated from Hoh Xil possesses strong stress tolerance, enabling it to propagate under high salinity (13%) and alkalinity (pH 10.0) conditions. In addition, KKD1 exhibits promising biocontrol activity against plant pathogens. To further explore these traits at the genomic level, we performed whole-genome sequencing and analysis. The taxonomic identification according to the average nucleotide identity based on BLAST revealed that KKD1 belongs to <i>Bacillus halotolerans</i>. Genetic screening of KKD1 revealed that its stress resistance mechanism depends on osmotic equilibrium, membrane transportation, and the regulation of ion balance under salt and alkaline stress. The expression of genes involved in these pathways was analyzed using real-time quantitative PCR. The presence of different gene clusters encoding antimicrobial secondary metabolites indicated the various pathways by which KKD1 suppresses phytopathogenic growth. Moreover, the lipopeptides surfactin and fengycin were identified as being significant antifungal components of KKD1. Through comparative genomics analysis, we noticed that KKD1 harbored specific genes involved in stress resistance and biocontrol, thus providing a new perspective on the genomic features of the extremophilic <i>Bacillus</i> species. |
| first_indexed | 2024-03-10T06:43:14Z |
| format | Article |
| id | doaj.art-39e90e95d3554efbb965f6c36e635631 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T06:43:14Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-39e90e95d3554efbb965f6c36e6356312023-11-22T17:28:43ZengMDPI AGBiology2079-77372021-10-011010103010.3390/biology10101030Genomic Features and Molecular Function of a Novel Stress-Tolerant <i>Bacillus halotolerans</i> Strain Isolated from an Extreme EnvironmentXiaohui Wu0Huijun Wu1Ruoyi Wang2Zhengqi Wang3Yaming Zhang4Qin Gu5Ayaz Farzand6Xue Yang7Mikhail Semenov8Rainer Borriss9Yongli Xie10Xuewen Gao11Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Grassland Science, College of Agricultural and Husbandry, Qinghai University, Xining 810016, ChinaDepartment of Soil Biology and Biochemistry, Dokuchaev Soil Science Institute, 119017 Moscow, RussiaInstitut für Biologie, Humboldt Universität Berlin, 10115 Berlin, GermanyDepartment of Grassland Science, College of Agricultural and Husbandry, Qinghai University, Xining 810016, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDue to its topographical position and climatic conditions, the Qinghai–Tibet Plateau possesses abundant microorganism resources. The extremophilic strain KKD1 isolated from Hoh Xil possesses strong stress tolerance, enabling it to propagate under high salinity (13%) and alkalinity (pH 10.0) conditions. In addition, KKD1 exhibits promising biocontrol activity against plant pathogens. To further explore these traits at the genomic level, we performed whole-genome sequencing and analysis. The taxonomic identification according to the average nucleotide identity based on BLAST revealed that KKD1 belongs to <i>Bacillus halotolerans</i>. Genetic screening of KKD1 revealed that its stress resistance mechanism depends on osmotic equilibrium, membrane transportation, and the regulation of ion balance under salt and alkaline stress. The expression of genes involved in these pathways was analyzed using real-time quantitative PCR. The presence of different gene clusters encoding antimicrobial secondary metabolites indicated the various pathways by which KKD1 suppresses phytopathogenic growth. Moreover, the lipopeptides surfactin and fengycin were identified as being significant antifungal components of KKD1. Through comparative genomics analysis, we noticed that KKD1 harbored specific genes involved in stress resistance and biocontrol, thus providing a new perspective on the genomic features of the extremophilic <i>Bacillus</i> species.https://www.mdpi.com/2079-7737/10/10/1030Qinghai–Tibet Plateau<i>Bacillus halotolerans</i>stress tolerancebiocontrolgenomic featurescomparative genomic analysis |
| spellingShingle | Xiaohui Wu Huijun Wu Ruoyi Wang Zhengqi Wang Yaming Zhang Qin Gu Ayaz Farzand Xue Yang Mikhail Semenov Rainer Borriss Yongli Xie Xuewen Gao Genomic Features and Molecular Function of a Novel Stress-Tolerant <i>Bacillus halotolerans</i> Strain Isolated from an Extreme Environment Biology Qinghai–Tibet Plateau <i>Bacillus halotolerans</i> stress tolerance biocontrol genomic features comparative genomic analysis |
| title | Genomic Features and Molecular Function of a Novel Stress-Tolerant <i>Bacillus halotolerans</i> Strain Isolated from an Extreme Environment |
| title_full | Genomic Features and Molecular Function of a Novel Stress-Tolerant <i>Bacillus halotolerans</i> Strain Isolated from an Extreme Environment |
| title_fullStr | Genomic Features and Molecular Function of a Novel Stress-Tolerant <i>Bacillus halotolerans</i> Strain Isolated from an Extreme Environment |
| title_full_unstemmed | Genomic Features and Molecular Function of a Novel Stress-Tolerant <i>Bacillus halotolerans</i> Strain Isolated from an Extreme Environment |
| title_short | Genomic Features and Molecular Function of a Novel Stress-Tolerant <i>Bacillus halotolerans</i> Strain Isolated from an Extreme Environment |
| title_sort | genomic features and molecular function of a novel stress tolerant i bacillus halotolerans i strain isolated from an extreme environment |
| topic | Qinghai–Tibet Plateau <i>Bacillus halotolerans</i> stress tolerance biocontrol genomic features comparative genomic analysis |
| url | https://www.mdpi.com/2079-7737/10/10/1030 |
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