A New Vestibular Stimulation Mode for Motion Sickness With Emphatic Analysis of Pica
Motion sickness (MS) was frequently introduced for rodents in research work through passive motion that disturbed vestibular signals in the presence of visual and aleatory, proprioceptive inputs. Inducement of MS in this way causes conflicting signals that activate intermixed neural circuits represe...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.882695/full |
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| author | Zhi-Hao Zhang Zhi-Hao Zhang Li-Peng Liu Yan Fang Xiao-Cheng Wang Wei Wang Wei Wang Ying-Shing Chan Lu Wang Hui Li Yun-Qing Li Fu-Xing Zhang |
| author_facet | Zhi-Hao Zhang Zhi-Hao Zhang Li-Peng Liu Yan Fang Xiao-Cheng Wang Wei Wang Wei Wang Ying-Shing Chan Lu Wang Hui Li Yun-Qing Li Fu-Xing Zhang |
| author_sort | Zhi-Hao Zhang |
| collection | DOAJ |
| description | Motion sickness (MS) was frequently introduced for rodents in research work through passive motion that disturbed vestibular signals in the presence of visual and aleatory, proprioceptive inputs. Inducement of MS in this way causes conflicting signals that activate intermixed neural circuits representing multimodal stimulation. From reductionism, a lab setup to elicit rat MS via vestibular stimulation was configured in the present study for MS study in connection with dissection of the central vestibular component causally underlying MS. The individual animal was blinded to light with a custom-made restrainer, and positioned at an inclination of 30° for otolith organs to receive unusual actions by gravitoinertial vector. Following a 2-h double-axis (earth–vertical) rotation involving angular acceleration/deceleration, a suit of behaviors characterizing the MS was observed to be significantly changed including pica (eating non-nutritive substance like kaolin), conditioned taste avoidance and locomotion (p < 0.05). Notably, for the statistical hypothesis testing, the utility of net increased amount of kaolin consumption as independent variables in data processing was expounded. In addition, Fos-immunostained neurons in vestibular nucleus complex were significantly increased in number, suggesting the rotation-induced MS was closely related to the vestibular activation. In conclusion, our work indicated that the present setup could effectively elicit the MS by disturbing vestibular signals in rat in the context of well-controlled proprioceptive inputs and lack of visual afference. |
| first_indexed | 2024-04-13T04:08:40Z |
| format | Article |
| id | doaj.art-39ef322bdff34f48bab3cfee27ab671a |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-04-13T04:08:40Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-39ef322bdff34f48bab3cfee27ab671a2022-12-22T03:03:10ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532022-05-011610.3389/fnbeh.2022.882695882695A New Vestibular Stimulation Mode for Motion Sickness With Emphatic Analysis of PicaZhi-Hao Zhang0Zhi-Hao Zhang1Li-Peng Liu2Yan Fang3Xiao-Cheng Wang4Wei Wang5Wei Wang6Ying-Shing Chan7Lu Wang8Hui Li9Yun-Qing Li10Fu-Xing Zhang11Department of Human Anatomy, Histology and Embryology & K.K. Leung Brain Research Centre, School of Basic Medicine, Fourth Military Medical University, Xi’an, ChinaDepartment of Anatomy, Medical College, Yan’an University, Yan’an, ChinaDepartment of Human Anatomy, Histology and Embryology & K.K. Leung Brain Research Centre, School of Basic Medicine, Fourth Military Medical University, Xi’an, ChinaDepartment of Human Anatomy, Histology and Embryology & K.K. Leung Brain Research Centre, School of Basic Medicine, Fourth Military Medical University, Xi’an, ChinaCenter of Clinical Aerospace Medicine, School of Aerospace Medicine, Fourth Military Medical University, Xi’an, ChinaDepartment of Human Anatomy, Histology and Embryology & K.K. Leung Brain Research Centre, School of Basic Medicine, Fourth Military Medical University, Xi’an, ChinaDepartment of Pharmacology, Xi’an Biomedicine College, Xi’an, ChinaSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaDepartment of Anatomy, Medical College, Yan’an University, Yan’an, ChinaDepartment of Human Anatomy, Histology and Embryology & K.K. Leung Brain Research Centre, School of Basic Medicine, Fourth Military Medical University, Xi’an, ChinaDepartment of Human Anatomy, Histology and Embryology & K.K. Leung Brain Research Centre, School of Basic Medicine, Fourth Military Medical University, Xi’an, ChinaDepartment of Human Anatomy, Histology and Embryology & K.K. Leung Brain Research Centre, School of Basic Medicine, Fourth Military Medical University, Xi’an, ChinaMotion sickness (MS) was frequently introduced for rodents in research work through passive motion that disturbed vestibular signals in the presence of visual and aleatory, proprioceptive inputs. Inducement of MS in this way causes conflicting signals that activate intermixed neural circuits representing multimodal stimulation. From reductionism, a lab setup to elicit rat MS via vestibular stimulation was configured in the present study for MS study in connection with dissection of the central vestibular component causally underlying MS. The individual animal was blinded to light with a custom-made restrainer, and positioned at an inclination of 30° for otolith organs to receive unusual actions by gravitoinertial vector. Following a 2-h double-axis (earth–vertical) rotation involving angular acceleration/deceleration, a suit of behaviors characterizing the MS was observed to be significantly changed including pica (eating non-nutritive substance like kaolin), conditioned taste avoidance and locomotion (p < 0.05). Notably, for the statistical hypothesis testing, the utility of net increased amount of kaolin consumption as independent variables in data processing was expounded. In addition, Fos-immunostained neurons in vestibular nucleus complex were significantly increased in number, suggesting the rotation-induced MS was closely related to the vestibular activation. In conclusion, our work indicated that the present setup could effectively elicit the MS by disturbing vestibular signals in rat in the context of well-controlled proprioceptive inputs and lack of visual afference.https://www.frontiersin.org/articles/10.3389/fnbeh.2022.882695/fullhypothesis testingmotion sicknesspicaratrotationconditioned taste avoidance |
| spellingShingle | Zhi-Hao Zhang Zhi-Hao Zhang Li-Peng Liu Yan Fang Xiao-Cheng Wang Wei Wang Wei Wang Ying-Shing Chan Lu Wang Hui Li Yun-Qing Li Fu-Xing Zhang A New Vestibular Stimulation Mode for Motion Sickness With Emphatic Analysis of Pica Frontiers in Behavioral Neuroscience hypothesis testing motion sickness pica rat rotation conditioned taste avoidance |
| title | A New Vestibular Stimulation Mode for Motion Sickness With Emphatic Analysis of Pica |
| title_full | A New Vestibular Stimulation Mode for Motion Sickness With Emphatic Analysis of Pica |
| title_fullStr | A New Vestibular Stimulation Mode for Motion Sickness With Emphatic Analysis of Pica |
| title_full_unstemmed | A New Vestibular Stimulation Mode for Motion Sickness With Emphatic Analysis of Pica |
| title_short | A New Vestibular Stimulation Mode for Motion Sickness With Emphatic Analysis of Pica |
| title_sort | new vestibular stimulation mode for motion sickness with emphatic analysis of pica |
| topic | hypothesis testing motion sickness pica rat rotation conditioned taste avoidance |
| url | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.882695/full |
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