Phytochemical analysis, UPLC-ESI-Orbitrap-MS analysis, biological activity, and toxicity of extracts from Tripleurospermum limosum (Maxim.) Pobed
Tripleurospermum limosum (TL) has been used in folk medicine to treat gastritis. Toward the further development and use of TL, we report the phytochemical profiling, determination of active components, and antioxidant and enzyme inhibitory activities of TL. Nineteen compounds were identified by ultr...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-05-01
|
| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535222001137 |
| _version_ | 1811291439280160768 |
|---|---|
| author | Meihua Chen Xu He Hui Sun Yue Sun Li Li Junyi Zhu Guangqing Xia Xin Guo Hao Zang |
| author_facet | Meihua Chen Xu He Hui Sun Yue Sun Li Li Junyi Zhu Guangqing Xia Xin Guo Hao Zang |
| author_sort | Meihua Chen |
| collection | DOAJ |
| description | Tripleurospermum limosum (TL) has been used in folk medicine to treat gastritis. Toward the further development and use of TL, we report the phytochemical profiling, determination of active components, and antioxidant and enzyme inhibitory activities of TL. Nineteen compounds were identified by ultra-performance liquid chromatography-electrospray ionization-orbitrap-mass spectrometry for the first time in this plant. Phytochemical studies indicated that TL contained 11 types of phytochemicals. The active components [total carbohydrate content (TCC), total protein content (TProC), total triterpenoid content (TTC), total phenolic content (TPheC), total flavonoid content (TFC), total phenolic acid content (TPAC), condensed tannin content (CTC), and gallotannin content (GC)] of eight different solvent extracts were determined by ultraviolet–visible spectrophotometry. Aqueous extract had highest TProC, TPheC, and GC values. Methanol extract exhibited highest TCC and TFC values. Ethanol extract showed highest TPAC and CTC values and dichloromethane extract exhibited highest TTC value. Methanol extract showed strongest ability to scavenge 2,2-diphenyl-1-picrylhydrazyl radicals, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt cation radicals, and hydroxyl radicals, and also exhibited highest antioxidant activity in ferric-reducing antioxidant power and cupric ion-reducing antioxidant capacity assays. Best iron and copper chelating activity and H2O2 scavenging ability were shown by aqueous extract. Ethanol extract showed strongest ability to scavenge superoxide radicals and effectively prevent β-carotene bleaching. Acetone extract had highest inhibitory activity toward α-glucosidase, α-amylase, and xanthine oxidase. Ethyl ether extract had highest inhibitory activity towards urease and angiotensin converting enzyme. Aqueous and ethanol extracts had strongest inhibitory activity toward acetylcholinesterase. Methanol extract showed highest inhibitory activity toward tyrosinase. Methanol extract showed good stability and antioxidant capacity during heating, at different pH values, and after in vitro digestion and had low toxicity. The efficacy of methanol extract in stabilizing olive and sunflower oils was studied, the results suggested that methanol extract had a protective effect on the primary oxidation of the two oils. TL may be useful as a source of active components for application in human nutrition and/or phytomedicine and methanol extract of TL could be used as a natural oil stabiliser. |
| first_indexed | 2024-04-13T04:29:24Z |
| format | Article |
| id | doaj.art-39f0f84d1677492db02362be6aaf3e13 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-13T04:29:24Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-39f0f84d1677492db02362be6aaf3e132022-12-22T03:02:23ZengElsevierArabian Journal of Chemistry1878-53522022-05-01155103797Phytochemical analysis, UPLC-ESI-Orbitrap-MS analysis, biological activity, and toxicity of extracts from Tripleurospermum limosum (Maxim.) PobedMeihua Chen0Xu He1Hui Sun2Yue Sun3Li Li4Junyi Zhu5Guangqing Xia6Xin Guo7Hao Zang8Tonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, China; Yanbian University, College of Medicine, Yanji 133000, ChinaTonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, China; Yanbian University, College of Medicine, Yanji 133000, ChinaTonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, ChinaTonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, China; Yanbian University, College of Medicine, Yanji 133000, ChinaTonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, China; Corresponding authors at: Tonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, China.Tonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, China; Key Laboratory of Evaluation and Application of Changbai Mountain Biological Gerplasm Resources of Jilin Province, Tonghua 134002, ChinaTonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, China; Yanbian University, College of Medicine, Yanji 133000, China; Key Laboratory of Evaluation and Application of Changbai Mountain Biological Gerplasm Resources of Jilin Province, Tonghua 134002, China; Corresponding authors at: Tonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, China.Tonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, ChinaTonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, China; Yanbian University, College of Medicine, Yanji 133000, China; Key Laboratory of Evaluation and Application of Changbai Mountain Biological Gerplasm Resources of Jilin Province, Tonghua 134002, China; Corresponding authors at: Tonghua Normal University, School of Pharmacy and Medicine, Green Medicinal Chemistry Laboratory, Tonghua 134002, China.Tripleurospermum limosum (TL) has been used in folk medicine to treat gastritis. Toward the further development and use of TL, we report the phytochemical profiling, determination of active components, and antioxidant and enzyme inhibitory activities of TL. Nineteen compounds were identified by ultra-performance liquid chromatography-electrospray ionization-orbitrap-mass spectrometry for the first time in this plant. Phytochemical studies indicated that TL contained 11 types of phytochemicals. The active components [total carbohydrate content (TCC), total protein content (TProC), total triterpenoid content (TTC), total phenolic content (TPheC), total flavonoid content (TFC), total phenolic acid content (TPAC), condensed tannin content (CTC), and gallotannin content (GC)] of eight different solvent extracts were determined by ultraviolet–visible spectrophotometry. Aqueous extract had highest TProC, TPheC, and GC values. Methanol extract exhibited highest TCC and TFC values. Ethanol extract showed highest TPAC and CTC values and dichloromethane extract exhibited highest TTC value. Methanol extract showed strongest ability to scavenge 2,2-diphenyl-1-picrylhydrazyl radicals, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt cation radicals, and hydroxyl radicals, and also exhibited highest antioxidant activity in ferric-reducing antioxidant power and cupric ion-reducing antioxidant capacity assays. Best iron and copper chelating activity and H2O2 scavenging ability were shown by aqueous extract. Ethanol extract showed strongest ability to scavenge superoxide radicals and effectively prevent β-carotene bleaching. Acetone extract had highest inhibitory activity toward α-glucosidase, α-amylase, and xanthine oxidase. Ethyl ether extract had highest inhibitory activity towards urease and angiotensin converting enzyme. Aqueous and ethanol extracts had strongest inhibitory activity toward acetylcholinesterase. Methanol extract showed highest inhibitory activity toward tyrosinase. Methanol extract showed good stability and antioxidant capacity during heating, at different pH values, and after in vitro digestion and had low toxicity. The efficacy of methanol extract in stabilizing olive and sunflower oils was studied, the results suggested that methanol extract had a protective effect on the primary oxidation of the two oils. TL may be useful as a source of active components for application in human nutrition and/or phytomedicine and methanol extract of TL could be used as a natural oil stabiliser.http://www.sciencedirect.com/science/article/pii/S1878535222001137Tripleurospermum limosumPhytochemical analysisUPLC-ESI-Orbitrap-MSAntioxidant activityEnzyme-inhibitory activityStability studies |
| spellingShingle | Meihua Chen Xu He Hui Sun Yue Sun Li Li Junyi Zhu Guangqing Xia Xin Guo Hao Zang Phytochemical analysis, UPLC-ESI-Orbitrap-MS analysis, biological activity, and toxicity of extracts from Tripleurospermum limosum (Maxim.) Pobed Arabian Journal of Chemistry Tripleurospermum limosum Phytochemical analysis UPLC-ESI-Orbitrap-MS Antioxidant activity Enzyme-inhibitory activity Stability studies |
| title | Phytochemical analysis, UPLC-ESI-Orbitrap-MS analysis, biological activity, and toxicity of extracts from Tripleurospermum limosum (Maxim.) Pobed |
| title_full | Phytochemical analysis, UPLC-ESI-Orbitrap-MS analysis, biological activity, and toxicity of extracts from Tripleurospermum limosum (Maxim.) Pobed |
| title_fullStr | Phytochemical analysis, UPLC-ESI-Orbitrap-MS analysis, biological activity, and toxicity of extracts from Tripleurospermum limosum (Maxim.) Pobed |
| title_full_unstemmed | Phytochemical analysis, UPLC-ESI-Orbitrap-MS analysis, biological activity, and toxicity of extracts from Tripleurospermum limosum (Maxim.) Pobed |
| title_short | Phytochemical analysis, UPLC-ESI-Orbitrap-MS analysis, biological activity, and toxicity of extracts from Tripleurospermum limosum (Maxim.) Pobed |
| title_sort | phytochemical analysis uplc esi orbitrap ms analysis biological activity and toxicity of extracts from tripleurospermum limosum maxim pobed |
| topic | Tripleurospermum limosum Phytochemical analysis UPLC-ESI-Orbitrap-MS Antioxidant activity Enzyme-inhibitory activity Stability studies |
| url | http://www.sciencedirect.com/science/article/pii/S1878535222001137 |
| work_keys_str_mv | AT meihuachen phytochemicalanalysisuplcesiorbitrapmsanalysisbiologicalactivityandtoxicityofextractsfromtripleurospermumlimosummaximpobed AT xuhe phytochemicalanalysisuplcesiorbitrapmsanalysisbiologicalactivityandtoxicityofextractsfromtripleurospermumlimosummaximpobed AT huisun phytochemicalanalysisuplcesiorbitrapmsanalysisbiologicalactivityandtoxicityofextractsfromtripleurospermumlimosummaximpobed AT yuesun phytochemicalanalysisuplcesiorbitrapmsanalysisbiologicalactivityandtoxicityofextractsfromtripleurospermumlimosummaximpobed AT lili phytochemicalanalysisuplcesiorbitrapmsanalysisbiologicalactivityandtoxicityofextractsfromtripleurospermumlimosummaximpobed AT junyizhu phytochemicalanalysisuplcesiorbitrapmsanalysisbiologicalactivityandtoxicityofextractsfromtripleurospermumlimosummaximpobed AT guangqingxia phytochemicalanalysisuplcesiorbitrapmsanalysisbiologicalactivityandtoxicityofextractsfromtripleurospermumlimosummaximpobed AT xinguo phytochemicalanalysisuplcesiorbitrapmsanalysisbiologicalactivityandtoxicityofextractsfromtripleurospermumlimosummaximpobed AT haozang phytochemicalanalysisuplcesiorbitrapmsanalysisbiologicalactivityandtoxicityofextractsfromtripleurospermumlimosummaximpobed |