Evaluation of post-natal angiogenesis in a mouse hind limb ischemia model
Summary: Hind limb ischemia is a useful model to assess metabolic and cellular responses. Here, we present a protocol for evaluating post-natal angiogenesis in a mouse hind limb ischemia model. We describe steps to induce a severe restriction of blood supply of the femoral artery and vein that mimic...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723001909 |
| _version_ | 1797844246611361792 |
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| author | Cristina Arce Recatalá Mattia Albiero Massimo Mattia Santoro |
| author_facet | Cristina Arce Recatalá Mattia Albiero Massimo Mattia Santoro |
| author_sort | Cristina Arce Recatalá |
| collection | DOAJ |
| description | Summary: Hind limb ischemia is a useful model to assess metabolic and cellular responses. Here, we present a protocol for evaluating post-natal angiogenesis in a mouse hind limb ischemia model. We describe steps to induce a severe restriction of blood supply of the femoral artery and vein that mimics the real-life scenario observed in clinical settings. We then detail procedures for follow-up laser Doppler imaging to compare post-ischemic responses of four different mouse strains in their capacity to trigger compensatory arteriogenesis.For complete details on the use and execution of this protocol, please refer to Oberkersch et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-09T17:20:20Z |
| format | Article |
| id | doaj.art-39f17197fdb946648ee14fd7431f3234 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-09T17:20:20Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-39f17197fdb946648ee14fd7431f32342023-04-19T04:23:20ZengElsevierSTAR Protocols2666-16672023-06-0142102232Evaluation of post-natal angiogenesis in a mouse hind limb ischemia modelCristina Arce Recatalá0Mattia Albiero1Massimo Mattia Santoro2Laboratory of Angiogenesis and Cancer Metabolism, DiBio, University of Padua, Padua, Italy; Veneto Institute of Molecular Medicine (VIMM), University of Padova, Padua, ItalyDepartment of Medicine (DIMED), University of Padova, Padua, Italy; Veneto Institute of Molecular Medicine (VIMM), University of Padova, Padua, ItalyLaboratory of Angiogenesis and Cancer Metabolism, DiBio, University of Padua, Padua, Italy; Veneto Institute of Molecular Medicine (VIMM), University of Padova, Padua, Italy; Corresponding authorSummary: Hind limb ischemia is a useful model to assess metabolic and cellular responses. Here, we present a protocol for evaluating post-natal angiogenesis in a mouse hind limb ischemia model. We describe steps to induce a severe restriction of blood supply of the femoral artery and vein that mimics the real-life scenario observed in clinical settings. We then detail procedures for follow-up laser Doppler imaging to compare post-ischemic responses of four different mouse strains in their capacity to trigger compensatory arteriogenesis.For complete details on the use and execution of this protocol, please refer to Oberkersch et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723001909Health SciencesMetabolismMicroscopyModel Organisms |
| spellingShingle | Cristina Arce Recatalá Mattia Albiero Massimo Mattia Santoro Evaluation of post-natal angiogenesis in a mouse hind limb ischemia model STAR Protocols Health Sciences Metabolism Microscopy Model Organisms |
| title | Evaluation of post-natal angiogenesis in a mouse hind limb ischemia model |
| title_full | Evaluation of post-natal angiogenesis in a mouse hind limb ischemia model |
| title_fullStr | Evaluation of post-natal angiogenesis in a mouse hind limb ischemia model |
| title_full_unstemmed | Evaluation of post-natal angiogenesis in a mouse hind limb ischemia model |
| title_short | Evaluation of post-natal angiogenesis in a mouse hind limb ischemia model |
| title_sort | evaluation of post natal angiogenesis in a mouse hind limb ischemia model |
| topic | Health Sciences Metabolism Microscopy Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723001909 |
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