Behavior of Neutrophil Granulocytes during Toxoplasma gondii Infection in the Central Nervous System
Cerebral toxoplasmosis is characterized by activation of brain resident cells and recruitment of specific immune cell subsets from the periphery to the central nervous system (CNS). Our studies revealed that the rapidly invaded Ly6G+ neutrophil granulocytes are an early non-lymphoid source of interf...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00259/full |
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| author | Aindrila Biswas Timothy French Henning P. Düsedau Nancy Mueller Monika Riek-Burchardt Anne Dudeck Ute Bank Thomas Schüler Ildiko Rita Dunay |
| author_facet | Aindrila Biswas Timothy French Henning P. Düsedau Nancy Mueller Monika Riek-Burchardt Anne Dudeck Ute Bank Thomas Schüler Ildiko Rita Dunay |
| author_sort | Aindrila Biswas |
| collection | DOAJ |
| description | Cerebral toxoplasmosis is characterized by activation of brain resident cells and recruitment of specific immune cell subsets from the periphery to the central nervous system (CNS). Our studies revealed that the rapidly invaded Ly6G+ neutrophil granulocytes are an early non-lymphoid source of interferon-gamma (IFN-γ), the cytokine known to be the major mediator of host resistance to Toxoplasma gondii (T. gondii). Upon selective depletion of Ly6G+ neutrophils, we detected reduced IFN-γ production and increased parasite burden in the CNS. Ablation of Ly6G+ cells resulted in diminished recruitment of Ly6Chi monocytes into the CNS, indicating a pronounced interplay. Additionally, we identified infiltrated Ly6G+ neutrophils to be a heterogeneous population. The Ly6G+CD62-LhiCXCR4+ subset released cathelicidin-related antimicrobial peptide (CRAMP), which can promote monocyte dynamics. On the other hand, the Ly6G+CD62-LloCXCR4+ subset produced IFN-γ to establish early inflammatory response. Collectively, our findings revealed that the recruited Ly6G+CXCR4+ neutrophil granulocytes display a heterogeneity in the CNS with a repertoire of effector functions crucial in parasite control and immune regulation upon experimental cerebral toxoplasmosis. |
| first_indexed | 2024-12-11T09:39:40Z |
| format | Article |
| id | doaj.art-39f3ddf9a2c0442db26fdcf2d97847cd |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-11T09:39:40Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-39f3ddf9a2c0442db26fdcf2d97847cd2022-12-22T01:12:42ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882017-06-01710.3389/fcimb.2017.00259262335Behavior of Neutrophil Granulocytes during Toxoplasma gondii Infection in the Central Nervous SystemAindrila Biswas0Timothy French1Henning P. Düsedau2Nancy Mueller3Monika Riek-Burchardt4Anne Dudeck5Ute Bank6Thomas Schüler7Ildiko Rita Dunay8Institute of Inflammation and Neurodegeneration, Otto-von-Guericke University MagdeburgMagdeburg, GermanyInstitute of Inflammation and Neurodegeneration, Otto-von-Guericke University MagdeburgMagdeburg, GermanyInstitute of Inflammation and Neurodegeneration, Otto-von-Guericke University MagdeburgMagdeburg, GermanyInstitute of Inflammation and Neurodegeneration, Otto-von-Guericke University MagdeburgMagdeburg, GermanyInstitute for Molecular and Clinical Immunology, Otto-von-Guericke University MagdeburgMagdeburg, GermanyInstitute for Molecular and Clinical Immunology, Otto-von-Guericke University MagdeburgMagdeburg, GermanyInstitute for Molecular and Clinical Immunology, Otto-von-Guericke University MagdeburgMagdeburg, GermanyInstitute for Molecular and Clinical Immunology, Otto-von-Guericke University MagdeburgMagdeburg, GermanyInstitute of Inflammation and Neurodegeneration, Otto-von-Guericke University MagdeburgMagdeburg, GermanyCerebral toxoplasmosis is characterized by activation of brain resident cells and recruitment of specific immune cell subsets from the periphery to the central nervous system (CNS). Our studies revealed that the rapidly invaded Ly6G+ neutrophil granulocytes are an early non-lymphoid source of interferon-gamma (IFN-γ), the cytokine known to be the major mediator of host resistance to Toxoplasma gondii (T. gondii). Upon selective depletion of Ly6G+ neutrophils, we detected reduced IFN-γ production and increased parasite burden in the CNS. Ablation of Ly6G+ cells resulted in diminished recruitment of Ly6Chi monocytes into the CNS, indicating a pronounced interplay. Additionally, we identified infiltrated Ly6G+ neutrophils to be a heterogeneous population. The Ly6G+CD62-LhiCXCR4+ subset released cathelicidin-related antimicrobial peptide (CRAMP), which can promote monocyte dynamics. On the other hand, the Ly6G+CD62-LloCXCR4+ subset produced IFN-γ to establish early inflammatory response. Collectively, our findings revealed that the recruited Ly6G+CXCR4+ neutrophil granulocytes display a heterogeneity in the CNS with a repertoire of effector functions crucial in parasite control and immune regulation upon experimental cerebral toxoplasmosis.http://journal.frontiersin.org/article/10.3389/fcimb.2017.00259/fullToxoplasma gondiineutrophil granulocytescerebral toxoplasmosisneuroinflammationneutrophil Infiltration |
| spellingShingle | Aindrila Biswas Timothy French Henning P. Düsedau Nancy Mueller Monika Riek-Burchardt Anne Dudeck Ute Bank Thomas Schüler Ildiko Rita Dunay Behavior of Neutrophil Granulocytes during Toxoplasma gondii Infection in the Central Nervous System Frontiers in Cellular and Infection Microbiology Toxoplasma gondii neutrophil granulocytes cerebral toxoplasmosis neuroinflammation neutrophil Infiltration |
| title | Behavior of Neutrophil Granulocytes during Toxoplasma gondii Infection in the Central Nervous System |
| title_full | Behavior of Neutrophil Granulocytes during Toxoplasma gondii Infection in the Central Nervous System |
| title_fullStr | Behavior of Neutrophil Granulocytes during Toxoplasma gondii Infection in the Central Nervous System |
| title_full_unstemmed | Behavior of Neutrophil Granulocytes during Toxoplasma gondii Infection in the Central Nervous System |
| title_short | Behavior of Neutrophil Granulocytes during Toxoplasma gondii Infection in the Central Nervous System |
| title_sort | behavior of neutrophil granulocytes during toxoplasma gondii infection in the central nervous system |
| topic | Toxoplasma gondii neutrophil granulocytes cerebral toxoplasmosis neuroinflammation neutrophil Infiltration |
| url | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00259/full |
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