MCF-7 and its Multidrug Resistant Variant MCF-7/ADR Overcome TNF Cytotoxicity through Prevention of Reactive Oxygen Species Accumulation
Background: Signal transduction of numerous cytokines and growth factors are mediated by reactive oxygen species (ROS). Tumor necrosis factor-α (TNF-α) have stimulated accumulation of ROS in various in vitro studies. MCF-7 and its Adriamycin resistant variant MCF-7/ADR are resistant against TNF-α cy...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2019-06-01
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| Series: | Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://ps.tbzmed.ac.ir/PDF/Pharm-31751 |
| _version_ | 1818619038530535424 |
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| author | Morteza Ghandadi Javad Behravan Samira Biabani Sara Abbaspor Fatemeh Mosaffa |
| author_facet | Morteza Ghandadi Javad Behravan Samira Biabani Sara Abbaspor Fatemeh Mosaffa |
| author_sort | Morteza Ghandadi |
| collection | DOAJ |
| description | Background: Signal transduction of numerous cytokines and growth factors are mediated by reactive oxygen species (ROS). Tumor necrosis factor-α (TNF-α) have stimulated accumulation of ROS in various in vitro studies. MCF-7 and its Adriamycin resistant variant MCF-7/ADR are resistant against TNF-α cytotoxicity. Role of ROS in the resistance of MCF-7 and MCF-7/ADR cells was investigated. Methods: ROS accumulation and viability in MCF-7 and MCF-7/ADR after TNF-α exposure was evaluated using 2',7'-dichlorofluorescein diacetate (DCFH-DA) as a fluorescent probe and 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide (MTT) cytotoxicity assay respectively. Results: ROS level did not change significantly after TNF-α exposure. Induction of ROS accumulation along with TNF-α treatment sensitized these cells to TNF-α toxicity. Conclusion: It can be concluded that lack of ROS accumulation following TNF-α exposure is involved at least by part in the resistance of MCF-7 and its drug resistant derivative MCF-7/ADR cells to TNF-α cytotoxicity. |
| first_indexed | 2024-12-16T17:31:08Z |
| format | Article |
| id | doaj.art-39f6793ffbef4838837ade80521a97a7 |
| institution | Directory Open Access Journal |
| issn | 2383-2886 |
| language | English |
| last_indexed | 2024-12-16T17:31:08Z |
| publishDate | 2019-06-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Pharmaceutical Sciences |
| spelling | doaj.art-39f6793ffbef4838837ade80521a97a72022-12-21T22:22:56ZengTabriz University of Medical SciencesPharmaceutical Sciences2383-28862019-06-0125211812310.15171/PS.2019.18Pharm-31751MCF-7 and its Multidrug Resistant Variant MCF-7/ADR Overcome TNF Cytotoxicity through Prevention of Reactive Oxygen Species AccumulationMorteza Ghandadi0Javad Behravan1Samira Biabani2Sara Abbaspor3Fatemeh Mosaffa4Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.Background: Signal transduction of numerous cytokines and growth factors are mediated by reactive oxygen species (ROS). Tumor necrosis factor-α (TNF-α) have stimulated accumulation of ROS in various in vitro studies. MCF-7 and its Adriamycin resistant variant MCF-7/ADR are resistant against TNF-α cytotoxicity. Role of ROS in the resistance of MCF-7 and MCF-7/ADR cells was investigated. Methods: ROS accumulation and viability in MCF-7 and MCF-7/ADR after TNF-α exposure was evaluated using 2',7'-dichlorofluorescein diacetate (DCFH-DA) as a fluorescent probe and 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide (MTT) cytotoxicity assay respectively. Results: ROS level did not change significantly after TNF-α exposure. Induction of ROS accumulation along with TNF-α treatment sensitized these cells to TNF-α toxicity. Conclusion: It can be concluded that lack of ROS accumulation following TNF-α exposure is involved at least by part in the resistance of MCF-7 and its drug resistant derivative MCF-7/ADR cells to TNF-α cytotoxicity.https://ps.tbzmed.ac.ir/PDF/Pharm-31751Breast cancerMCF-7Multidrug resistance;ROSTNF-α |
| spellingShingle | Morteza Ghandadi Javad Behravan Samira Biabani Sara Abbaspor Fatemeh Mosaffa MCF-7 and its Multidrug Resistant Variant MCF-7/ADR Overcome TNF Cytotoxicity through Prevention of Reactive Oxygen Species Accumulation Pharmaceutical Sciences Breast cancer MCF-7 Multidrug resistance; ROS TNF-α |
| title | MCF-7 and its Multidrug Resistant Variant MCF-7/ADR Overcome TNF Cytotoxicity through Prevention of Reactive Oxygen Species Accumulation |
| title_full | MCF-7 and its Multidrug Resistant Variant MCF-7/ADR Overcome TNF Cytotoxicity through Prevention of Reactive Oxygen Species Accumulation |
| title_fullStr | MCF-7 and its Multidrug Resistant Variant MCF-7/ADR Overcome TNF Cytotoxicity through Prevention of Reactive Oxygen Species Accumulation |
| title_full_unstemmed | MCF-7 and its Multidrug Resistant Variant MCF-7/ADR Overcome TNF Cytotoxicity through Prevention of Reactive Oxygen Species Accumulation |
| title_short | MCF-7 and its Multidrug Resistant Variant MCF-7/ADR Overcome TNF Cytotoxicity through Prevention of Reactive Oxygen Species Accumulation |
| title_sort | mcf 7 and its multidrug resistant variant mcf 7 adr overcome tnf cytotoxicity through prevention of reactive oxygen species accumulation |
| topic | Breast cancer MCF-7 Multidrug resistance; ROS TNF-α |
| url | https://ps.tbzmed.ac.ir/PDF/Pharm-31751 |
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