Transcriptome analysis and identification of genes associated with leaf crude protein content in foxtail millet [Setaria italica (L.) P. Beauv.]
Introduction: Spruce spider mite is a primary insect pest of Chinese chestnut in China and seriously influences its yield and quality. However, the current management against this mite is costly and poorly effective. In previous research, we bred several foxtail millet materials for interplanting wi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-01-01
|
| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1122212/full |
| _version_ | 1828057893912444928 |
|---|---|
| author | Yanjiao Cui Dan Liu Zilong Zhao Jing Zhang Suying Li Zhengli Liu |
| author_facet | Yanjiao Cui Dan Liu Zilong Zhao Jing Zhang Suying Li Zhengli Liu |
| author_sort | Yanjiao Cui |
| collection | DOAJ |
| description | Introduction: Spruce spider mite is a primary insect pest of Chinese chestnut in China and seriously influences its yield and quality. However, the current management against this mite is costly and poorly effective. In previous research, we bred several foxtail millet materials for interplanting with chestnut tree, and found that they had high levels of crude protein (CP) in leaves and attracted spruce spider mite to feed on the leaves, thereby reducing chestnut damage.Methods: In this study, four foxtail millet varieties with significant differences in leaf crude protein content were used for high-throughput sequencing and identification of genes associated with leaf crude protein content. Gene enrichment analyses were carried out to comprehend the functions of these genes and the biological processes in which they are involved. In addition, transcription factors (TFs) were evaluated.Results: 435 differentially expressed genes (DEGs) were identified, suggesting their potential role in crude protein accumulation. Some differentially expressed genes were found to be associated with nitrogen metabolism and ubiquitin-mediated proteolysis pathways. Moreover, we identified 40 TF genes categorized into 11 transcription factor families.Discussion: Our findings represent an important resource that clarifies the mechanisms of accumulation and control of leaf crude protein in foxtail millet, and provide an opportunity for suppression of spruce spider mite attack on Chinese chestnut by interplanting with foxtail millet varieties with high concentrations of leaf crude protein. |
| first_indexed | 2024-04-10T21:19:26Z |
| format | Article |
| id | doaj.art-39f6e8ba130345b3a30bcad9560eaabe |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-10T21:19:26Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-39f6e8ba130345b3a30bcad9560eaabe2023-01-20T06:42:51ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-01-011410.3389/fgene.2023.11222121122212Transcriptome analysis and identification of genes associated with leaf crude protein content in foxtail millet [Setaria italica (L.) P. Beauv.]Yanjiao Cui0Dan Liu1Zilong Zhao2Jing Zhang3Suying Li4Zhengli Liu5Department of Life Sciences, Tangshan Normal University, Tangshan, ChinaTianjin Key Laboratory of Crop Genetics and Breeding, Institute of Crop Sciences, Tianjin Academy of Agricultural Sciences, Tianjin, ChinaDepartment of Life Sciences, Tangshan Normal University, Tangshan, ChinaDepartment of Life Sciences, Tangshan Normal University, Tangshan, ChinaDepartment of Life Sciences, Tangshan Normal University, Tangshan, ChinaDepartment of Life Sciences, Tangshan Normal University, Tangshan, ChinaIntroduction: Spruce spider mite is a primary insect pest of Chinese chestnut in China and seriously influences its yield and quality. However, the current management against this mite is costly and poorly effective. In previous research, we bred several foxtail millet materials for interplanting with chestnut tree, and found that they had high levels of crude protein (CP) in leaves and attracted spruce spider mite to feed on the leaves, thereby reducing chestnut damage.Methods: In this study, four foxtail millet varieties with significant differences in leaf crude protein content were used for high-throughput sequencing and identification of genes associated with leaf crude protein content. Gene enrichment analyses were carried out to comprehend the functions of these genes and the biological processes in which they are involved. In addition, transcription factors (TFs) were evaluated.Results: 435 differentially expressed genes (DEGs) were identified, suggesting their potential role in crude protein accumulation. Some differentially expressed genes were found to be associated with nitrogen metabolism and ubiquitin-mediated proteolysis pathways. Moreover, we identified 40 TF genes categorized into 11 transcription factor families.Discussion: Our findings represent an important resource that clarifies the mechanisms of accumulation and control of leaf crude protein in foxtail millet, and provide an opportunity for suppression of spruce spider mite attack on Chinese chestnut by interplanting with foxtail millet varieties with high concentrations of leaf crude protein.https://www.frontiersin.org/articles/10.3389/fgene.2023.1122212/fullfoxtail milletChinese chestnutspruce spider mitecrude protein contenttranscriptome analysis |
| spellingShingle | Yanjiao Cui Dan Liu Zilong Zhao Jing Zhang Suying Li Zhengli Liu Transcriptome analysis and identification of genes associated with leaf crude protein content in foxtail millet [Setaria italica (L.) P. Beauv.] Frontiers in Genetics foxtail millet Chinese chestnut spruce spider mite crude protein content transcriptome analysis |
| title | Transcriptome analysis and identification of genes associated with leaf crude protein content in foxtail millet [Setaria italica (L.) P. Beauv.] |
| title_full | Transcriptome analysis and identification of genes associated with leaf crude protein content in foxtail millet [Setaria italica (L.) P. Beauv.] |
| title_fullStr | Transcriptome analysis and identification of genes associated with leaf crude protein content in foxtail millet [Setaria italica (L.) P. Beauv.] |
| title_full_unstemmed | Transcriptome analysis and identification of genes associated with leaf crude protein content in foxtail millet [Setaria italica (L.) P. Beauv.] |
| title_short | Transcriptome analysis and identification of genes associated with leaf crude protein content in foxtail millet [Setaria italica (L.) P. Beauv.] |
| title_sort | transcriptome analysis and identification of genes associated with leaf crude protein content in foxtail millet setaria italica l p beauv |
| topic | foxtail millet Chinese chestnut spruce spider mite crude protein content transcriptome analysis |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1122212/full |
| work_keys_str_mv | AT yanjiaocui transcriptomeanalysisandidentificationofgenesassociatedwithleafcrudeproteincontentinfoxtailmilletsetariaitalicalpbeauv AT danliu transcriptomeanalysisandidentificationofgenesassociatedwithleafcrudeproteincontentinfoxtailmilletsetariaitalicalpbeauv AT zilongzhao transcriptomeanalysisandidentificationofgenesassociatedwithleafcrudeproteincontentinfoxtailmilletsetariaitalicalpbeauv AT jingzhang transcriptomeanalysisandidentificationofgenesassociatedwithleafcrudeproteincontentinfoxtailmilletsetariaitalicalpbeauv AT suyingli transcriptomeanalysisandidentificationofgenesassociatedwithleafcrudeproteincontentinfoxtailmilletsetariaitalicalpbeauv AT zhengliliu transcriptomeanalysisandidentificationofgenesassociatedwithleafcrudeproteincontentinfoxtailmilletsetariaitalicalpbeauv |