Development of microsatellite markers for the genetic analysis of Magnaporthe grisea
An AG microsatellite-enriched genomic DNA library was constructed for Magnaporthe grisea (anamorph Pyricularia grisea), the causal agent of rice blast. Seventy-two DNA clones containing microsatellite repeats were isolated and sequenced in order to develop a series of new PCR-based molecular markers...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2000-01-01
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| Series: | Genetics and Molecular Biology |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400009 |
| _version_ | 1811339242723344384 |
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| author | Brondani Claudio Brondani Rosana Pereira Vianello Garrido Lucas da Ressurreição Ferreira Márcio Elias |
| author_facet | Brondani Claudio Brondani Rosana Pereira Vianello Garrido Lucas da Ressurreição Ferreira Márcio Elias |
| author_sort | Brondani Claudio |
| collection | DOAJ |
| description | An AG microsatellite-enriched genomic DNA library was constructed for Magnaporthe grisea (anamorph Pyricularia grisea), the causal agent of rice blast. Seventy-two DNA clones containing microsatellite repeats were isolated and sequenced in order to develop a series of new PCR-based molecular markers to be used in genetic studies of the fungus. Twenty-four of these clones were selected to design primer pairs for the PCR amplification of microsatellite alleles. Single spore cultures of M. grisea isolated from rice and wheat in Brazil, Colombia and China were genotyped at three microsatellite loci. Isolates from southern Brazil were predominantly monomorphic at the tested SSR loci, indicating a low level of genetic variability in these samples. However, seven alleles were observed at the MGM-1 locus in isolates from Central Brazil and at least nine alleles were detected at the same locus in a sample of Colombian isolates. Polymorphism analysis at SSR loci is a simple and direct approach for estimating the genetic diversity of M. grisea isolates and a powerful tool for studying M. grisea genetics. |
| first_indexed | 2024-04-13T18:23:24Z |
| format | Article |
| id | doaj.art-39f8b75830c647ebae245383ae0ccd03 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-04-13T18:23:24Z |
| publishDate | 2000-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-39f8b75830c647ebae245383ae0ccd032022-12-22T02:35:21ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852000-01-01234753762Development of microsatellite markers for the genetic analysis of Magnaporthe griseaBrondani ClaudioBrondani Rosana Pereira VianelloGarrido Lucas da RessurreiçãoFerreira Márcio EliasAn AG microsatellite-enriched genomic DNA library was constructed for Magnaporthe grisea (anamorph Pyricularia grisea), the causal agent of rice blast. Seventy-two DNA clones containing microsatellite repeats were isolated and sequenced in order to develop a series of new PCR-based molecular markers to be used in genetic studies of the fungus. Twenty-four of these clones were selected to design primer pairs for the PCR amplification of microsatellite alleles. Single spore cultures of M. grisea isolated from rice and wheat in Brazil, Colombia and China were genotyped at three microsatellite loci. Isolates from southern Brazil were predominantly monomorphic at the tested SSR loci, indicating a low level of genetic variability in these samples. However, seven alleles were observed at the MGM-1 locus in isolates from Central Brazil and at least nine alleles were detected at the same locus in a sample of Colombian isolates. Polymorphism analysis at SSR loci is a simple and direct approach for estimating the genetic diversity of M. grisea isolates and a powerful tool for studying M. grisea genetics.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400009 |
| spellingShingle | Brondani Claudio Brondani Rosana Pereira Vianello Garrido Lucas da Ressurreição Ferreira Márcio Elias Development of microsatellite markers for the genetic analysis of Magnaporthe grisea Genetics and Molecular Biology |
| title | Development of microsatellite markers for the genetic analysis of Magnaporthe grisea |
| title_full | Development of microsatellite markers for the genetic analysis of Magnaporthe grisea |
| title_fullStr | Development of microsatellite markers for the genetic analysis of Magnaporthe grisea |
| title_full_unstemmed | Development of microsatellite markers for the genetic analysis of Magnaporthe grisea |
| title_short | Development of microsatellite markers for the genetic analysis of Magnaporthe grisea |
| title_sort | development of microsatellite markers for the genetic analysis of magnaporthe grisea |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400009 |
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