Short-term treatment with Uncaria tomentosa aggravates the injury phenotype in mdx mice
Introduction: Uncaria tomentosa (Willd. ex Roem. & Schult.) DC. (Rubiaceae) or UT is a medicinal plant with antiviral, antimutagenic, anti-inflammatory and antioxidant properties. Duchenne muscular dystrophy (DMD) is a severe muscle wasting disease caused by mutations in the dystrophin gene; th...
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| Format: | Article |
| Language: | English |
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Faculdade de Medicina do ABC
2024-03-01
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| Series: | ABCS Health Sciences |
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| Online Access: | https://www.portalnepas.org.br/abcshs/article/view/2058 |
| _version_ | 1797265526597091328 |
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| author | David Feder Túlio de Almeida Hermes Lucas Prezotto Giordani Bruno Machado Bertassoli Giuliana Petri Fabio Perazzo Fernando Luiz Affonso Fonseca Alzira Alves de Siqueira Carvalho |
| author_facet | David Feder Túlio de Almeida Hermes Lucas Prezotto Giordani Bruno Machado Bertassoli Giuliana Petri Fabio Perazzo Fernando Luiz Affonso Fonseca Alzira Alves de Siqueira Carvalho |
| author_sort | David Feder |
| collection | DOAJ |
| description |
Introduction: Uncaria tomentosa (Willd. ex Roem. & Schult.) DC. (Rubiaceae) or UT is a medicinal plant with antiviral, antimutagenic, anti-inflammatory and antioxidant properties. Duchenne muscular dystrophy (DMD) is a severe muscle wasting disease caused by mutations in the dystrophin gene; this deficiency leads to sarcolemma instability, inflammation, muscle degeneration and fibrosis. Objective: Considering the importance of inflammation to dystrophy progression and the anti-inflammatory activity of UT, in the present study we evaluated whether oral administration of UT extract would ameliorate dystrophy in the mdx mice, a DMD model. Methods: Eight-week-old male mdx mice were submitted to 200 mg/kg body weight daily UT oral administration for 6 weeks. General histopathology was analysed, and muscle tumor necrosis factor α, transforming growth factor-β, myostatin and osteopontin transcript levels were assessed. The ability of mice to sustain limb tension to oppose their gravitational force was measured. Data were analysed with the unpaired Student’s t-test. Results: Morphologically, both untreated and UT-treated animals exhibited internalised nuclei, increased endomysial connective tissue and variations in muscle fibre diameters. Body weight and muscle strength were significantly reduced in the UT-treated animals. Blood creatine kinase was higher in UT-treated compared to untreated animals. In tibialis anterior, myostatin, transcript was more highly expressed in the UT-treated while in the diaphragm muscle, transforming growth factor-β transcripts were less expressed in the UT-treated. Conclusion: While previous studies identified anti-inflammatory, antiproliferative and anticarcinogenic UT effects, the extract indicates worsening of dystrophic muscles phenotype after short-term treatment in mdx mice.
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| first_indexed | 2024-04-25T00:46:12Z |
| format | Article |
| id | doaj.art-39f956ad9cb6487782c1f4bcd7bf9036 |
| institution | Directory Open Access Journal |
| issn | 2318-4965 2357-8114 |
| language | English |
| last_indexed | 2024-04-25T00:46:12Z |
| publishDate | 2024-03-01 |
| publisher | Faculdade de Medicina do ABC |
| record_format | Article |
| series | ABCS Health Sciences |
| spelling | doaj.art-39f956ad9cb6487782c1f4bcd7bf90362024-03-12T03:54:12ZengFaculdade de Medicina do ABCABCS Health Sciences2318-49652357-81142024-03-0110.7322/abcshs.2022018.2058Short-term treatment with Uncaria tomentosa aggravates the injury phenotype in mdx miceDavid Feder0Túlio de Almeida Hermes1Lucas Prezotto Giordani2Bruno Machado Bertassoli3Giuliana Petri4Fabio Perazzo5Fernando Luiz Affonso Fonseca6Alzira Alves de Siqueira Carvalho7Departamento de Morfologia e Fisiologia, Centro Universitário FMABC (FMABC) - Santo André (SP), BrazilDepartamento de Anatomia, Universidade Federal de Alfenas (UNIFAL) – Alfenas (MG), Brazil Departamento de Morfologia e Fisiologia, Centro Universitário FMABC (FMABC) - Santo André (SP), BrazilDepartamento de Morfologia e Fisiologia, Centro Universitário FMABC (FMABC) - Santo André (SP), BrazilDepartamento de Morfologia e Fisiologia, Centro Universitário FMABC (FMABC) - Santo André (SP), BrazilDepartamento de Ciências Exatas e da Terra, Universidade Federal de São Paulo (UNIFESP) - São Paulo (SP), BrazilDepartamento de Análises Clínicas, Centro Universitário FMABC (FMABC) - Santo André (SP), BrazilLaboratório de Doenças Neuromusculares, Centro Universitário FMABC (FMABC) - Santo André (SP), Brazil Introduction: Uncaria tomentosa (Willd. ex Roem. & Schult.) DC. (Rubiaceae) or UT is a medicinal plant with antiviral, antimutagenic, anti-inflammatory and antioxidant properties. Duchenne muscular dystrophy (DMD) is a severe muscle wasting disease caused by mutations in the dystrophin gene; this deficiency leads to sarcolemma instability, inflammation, muscle degeneration and fibrosis. Objective: Considering the importance of inflammation to dystrophy progression and the anti-inflammatory activity of UT, in the present study we evaluated whether oral administration of UT extract would ameliorate dystrophy in the mdx mice, a DMD model. Methods: Eight-week-old male mdx mice were submitted to 200 mg/kg body weight daily UT oral administration for 6 weeks. General histopathology was analysed, and muscle tumor necrosis factor α, transforming growth factor-β, myostatin and osteopontin transcript levels were assessed. The ability of mice to sustain limb tension to oppose their gravitational force was measured. Data were analysed with the unpaired Student’s t-test. Results: Morphologically, both untreated and UT-treated animals exhibited internalised nuclei, increased endomysial connective tissue and variations in muscle fibre diameters. Body weight and muscle strength were significantly reduced in the UT-treated animals. Blood creatine kinase was higher in UT-treated compared to untreated animals. In tibialis anterior, myostatin, transcript was more highly expressed in the UT-treated while in the diaphragm muscle, transforming growth factor-β transcripts were less expressed in the UT-treated. Conclusion: While previous studies identified anti-inflammatory, antiproliferative and anticarcinogenic UT effects, the extract indicates worsening of dystrophic muscles phenotype after short-term treatment in mdx mice. https://www.portalnepas.org.br/abcshs/article/view/2058Uncaria tomentosaDuchenne muscular dystrophymdx miceneuromuscularmyotoxic |
| spellingShingle | David Feder Túlio de Almeida Hermes Lucas Prezotto Giordani Bruno Machado Bertassoli Giuliana Petri Fabio Perazzo Fernando Luiz Affonso Fonseca Alzira Alves de Siqueira Carvalho Short-term treatment with Uncaria tomentosa aggravates the injury phenotype in mdx mice ABCS Health Sciences Uncaria tomentosa Duchenne muscular dystrophy mdx mice neuromuscular myotoxic |
| title | Short-term treatment with Uncaria tomentosa aggravates the injury phenotype in mdx mice |
| title_full | Short-term treatment with Uncaria tomentosa aggravates the injury phenotype in mdx mice |
| title_fullStr | Short-term treatment with Uncaria tomentosa aggravates the injury phenotype in mdx mice |
| title_full_unstemmed | Short-term treatment with Uncaria tomentosa aggravates the injury phenotype in mdx mice |
| title_short | Short-term treatment with Uncaria tomentosa aggravates the injury phenotype in mdx mice |
| title_sort | short term treatment with uncaria tomentosa aggravates the injury phenotype in mdx mice |
| topic | Uncaria tomentosa Duchenne muscular dystrophy mdx mice neuromuscular myotoxic |
| url | https://www.portalnepas.org.br/abcshs/article/view/2058 |
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