SEMA3B‐AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B‐dependent VEGF signaling pathway activation
Abstract Mounting evidence has demonstrated the considerable regulatory effects of long noncoding RNAs (lncRNAs) in the tumorigenesis and progression of various carcinomas. LncRNA Semaphorin 3B (SEMA3B) antisense RNA 1 (SEMA3B‐AS1) has been found to be dysregulated in a few carcinomas recently. Howe...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-10-01
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| Series: | MedComm |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mco2.365 |
| _version_ | 1827792913256415232 |
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| author | Yi‐Qing Wang Hui Chen Shuang Xu Cong‐Rui Liao Anran Xu Yue Han Min‐Hui Yang Li Zhao Sha‐Sha Hu Lan Wang Qing‐Yuan Li Ling‐Ying Zhan Yan‐Qing Ding Shuang Wang |
| author_facet | Yi‐Qing Wang Hui Chen Shuang Xu Cong‐Rui Liao Anran Xu Yue Han Min‐Hui Yang Li Zhao Sha‐Sha Hu Lan Wang Qing‐Yuan Li Ling‐Ying Zhan Yan‐Qing Ding Shuang Wang |
| author_sort | Yi‐Qing Wang |
| collection | DOAJ |
| description | Abstract Mounting evidence has demonstrated the considerable regulatory effects of long noncoding RNAs (lncRNAs) in the tumorigenesis and progression of various carcinomas. LncRNA Semaphorin 3B (SEMA3B) antisense RNA 1 (SEMA3B‐AS1) has been found to be dysregulated in a few carcinomas recently. However, its potential function and mechanism in colorectal carcinoma (CRC) have not yet been examined. Here we show that SEMA3B‐AS1 acts as a crucial regulator of CRC progression. We found that SEMA3B‐AS1 expression was downregulated in CRC cell lines and tissues. Downregulation of SEMA3B‐AS1 was significantly associated with poor survival in CRC patients. Overexpression of SEMA3B‐AS1 reduced the cell growth and metastasis of CRC in vivo and in vitro. In addition, SEMA3B‐AS1 promoted the expression of its sense‐cognate gene SEMA3B, a member of the Semaphorin family (SEMAs), by recruiting EP300 to induce H3K9 acetylation at the SEMA3B promoter. Furthermore, we proved that SEMA3B‐AS1 suppressed CRC angiogenesis by affecting the vascular endothelial growth factor signaling pathway activation which was regulated by the SEMA3B‐NRP1 axis. Our work unravels a novel mechanism of SEMA3B‐AS1 in the inhibition of CRC malignant progression and highlights its probability as a new promising diagnostic marker and therapeutic target for CRC interventions. |
| first_indexed | 2024-03-11T18:09:58Z |
| format | Article |
| id | doaj.art-39f9fc392329481e8265020e8b333bb7 |
| institution | Directory Open Access Journal |
| issn | 2688-2663 |
| language | English |
| last_indexed | 2024-03-11T18:09:58Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | MedComm |
| spelling | doaj.art-39f9fc392329481e8265020e8b333bb72023-10-16T15:20:40ZengWileyMedComm2688-26632023-10-0145n/an/a10.1002/mco2.365SEMA3B‐AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B‐dependent VEGF signaling pathway activationYi‐Qing Wang0Hui Chen1Shuang Xu2Cong‐Rui Liao3Anran Xu4Yue Han5Min‐Hui Yang6Li Zhao7Sha‐Sha Hu8Lan Wang9Qing‐Yuan Li10Ling‐Ying Zhan11Yan‐Qing Ding12Shuang Wang13Department of Pathology Nanfang Hospital Southern Medical University Guangzhou Guangdong ChinaDepartment of Pathology Nanfang Hospital Southern Medical University Guangzhou Guangdong ChinaDepartment of Pathology Nanfang Hospital Southern Medical University Guangzhou Guangdong ChinaDivision of Spine Surgery Department of Orthopaedics Nanfang Hospital Southern Medical University Guangzhou Guangdong ChinaDepartment of Pathology School of Basic Medical Sciences Southern Medical University Guangzhou Guangdong ChinaDepartment of Pathology School of Basic Medical Sciences Southern Medical University Guangzhou Guangdong ChinaDepartment of Pathology Nanfang Hospital Southern Medical University Guangzhou Guangdong ChinaDepartment of Pathology School of Basic Medical Sciences Southern Medical University Guangzhou Guangdong ChinaDepartment of Pathology School of Basic Medical Sciences Southern Medical University Guangzhou Guangdong ChinaDepartment of Pathology School of Basic Medical Sciences Southern Medical University Guangzhou Guangdong ChinaGuangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou Guangdong ChinaDepartment of Pathology School of Basic Medical Sciences Southern Medical University Guangzhou Guangdong ChinaDepartment of Pathology Nanfang Hospital Southern Medical University Guangzhou Guangdong ChinaDepartment of Pathology Nanfang Hospital Southern Medical University Guangzhou Guangdong ChinaAbstract Mounting evidence has demonstrated the considerable regulatory effects of long noncoding RNAs (lncRNAs) in the tumorigenesis and progression of various carcinomas. LncRNA Semaphorin 3B (SEMA3B) antisense RNA 1 (SEMA3B‐AS1) has been found to be dysregulated in a few carcinomas recently. However, its potential function and mechanism in colorectal carcinoma (CRC) have not yet been examined. Here we show that SEMA3B‐AS1 acts as a crucial regulator of CRC progression. We found that SEMA3B‐AS1 expression was downregulated in CRC cell lines and tissues. Downregulation of SEMA3B‐AS1 was significantly associated with poor survival in CRC patients. Overexpression of SEMA3B‐AS1 reduced the cell growth and metastasis of CRC in vivo and in vitro. In addition, SEMA3B‐AS1 promoted the expression of its sense‐cognate gene SEMA3B, a member of the Semaphorin family (SEMAs), by recruiting EP300 to induce H3K9 acetylation at the SEMA3B promoter. Furthermore, we proved that SEMA3B‐AS1 suppressed CRC angiogenesis by affecting the vascular endothelial growth factor signaling pathway activation which was regulated by the SEMA3B‐NRP1 axis. Our work unravels a novel mechanism of SEMA3B‐AS1 in the inhibition of CRC malignant progression and highlights its probability as a new promising diagnostic marker and therapeutic target for CRC interventions.https://doi.org/10.1002/mco2.365colorectal carcinomaepigenetic regulationSEMA3BSEMA3B‐AS1tumor angiogenesis |
| spellingShingle | Yi‐Qing Wang Hui Chen Shuang Xu Cong‐Rui Liao Anran Xu Yue Han Min‐Hui Yang Li Zhao Sha‐Sha Hu Lan Wang Qing‐Yuan Li Ling‐Ying Zhan Yan‐Qing Ding Shuang Wang SEMA3B‐AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B‐dependent VEGF signaling pathway activation MedComm colorectal carcinoma epigenetic regulation SEMA3B SEMA3B‐AS1 tumor angiogenesis |
| title | SEMA3B‐AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B‐dependent VEGF signaling pathway activation |
| title_full | SEMA3B‐AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B‐dependent VEGF signaling pathway activation |
| title_fullStr | SEMA3B‐AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B‐dependent VEGF signaling pathway activation |
| title_full_unstemmed | SEMA3B‐AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B‐dependent VEGF signaling pathway activation |
| title_short | SEMA3B‐AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B‐dependent VEGF signaling pathway activation |
| title_sort | sema3b as1 suppresses colorectal carcinoma progression by inhibiting semaphorin 3b dependent vegf signaling pathway activation |
| topic | colorectal carcinoma epigenetic regulation SEMA3B SEMA3B‐AS1 tumor angiogenesis |
| url | https://doi.org/10.1002/mco2.365 |
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