An Oxalate Transporter Gene, <i>AtOT</i>, Enhances Aluminum Tolerance in <i>Arabidopsis thaliana</i> by Regulating Oxalate Efflux
Secretion and efflux of oxalic acid from roots is an important aluminum detoxification mechanism for various plants; however, how this process is completed remains unclear. In this study, the candidate oxalate transporter gene <i>AtOT</i>, encoding 287 amino acids, was cloned and identif...
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2023-02-01
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author | Zongming Yang Pingjuan Zhao Xuehua Luo Wentao Peng Zifan Liu Guishui Xie Mengyue Wang Feng An |
author_facet | Zongming Yang Pingjuan Zhao Xuehua Luo Wentao Peng Zifan Liu Guishui Xie Mengyue Wang Feng An |
author_sort | Zongming Yang |
collection | DOAJ |
description | Secretion and efflux of oxalic acid from roots is an important aluminum detoxification mechanism for various plants; however, how this process is completed remains unclear. In this study, the candidate oxalate transporter gene <i>AtOT</i>, encoding 287 amino acids, was cloned and identified from <i>Arabidopsis thaliana</i>. <i>AtOT</i> was upregulated in response to aluminum stress at the transcriptional level, which was closely related to aluminum treatment concentration and time. The root growth of <i>Arabidopsis</i> was inhibited after knocking out <i>AtOT</i>, and this effect was amplified by aluminum stress. Yeast cells expressing <i>AtOT</i> enhanced oxalic acid resistance and aluminum tolerance, which was closely correlated with the secretion of oxalic acid by membrane vesicle transport. Collectively, these results underline an external exclusion mechanism of oxalate involving <i>AtOT</i> to enhance oxalic acid resistance and aluminum tolerance. |
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spelling | doaj.art-39fb426435894d218906200bfe302ad32023-11-17T07:49:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01245451610.3390/ijms24054516An Oxalate Transporter Gene, <i>AtOT</i>, Enhances Aluminum Tolerance in <i>Arabidopsis thaliana</i> by Regulating Oxalate EffluxZongming Yang0Pingjuan Zhao1Xuehua Luo2Wentao Peng3Zifan Liu4Guishui Xie5Mengyue Wang6Feng An7Hainan Danzhou Agro-Ecosystem National Observation and Research Station, Rubber Research Institute of Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, ChinaInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaHainan Danzhou Agro-Ecosystem National Observation and Research Station, Rubber Research Institute of Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaHainan Danzhou Agro-Ecosystem National Observation and Research Station, Rubber Research Institute of Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaHainan Danzhou Agro-Ecosystem National Observation and Research Station, Rubber Research Institute of Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaSecretion and efflux of oxalic acid from roots is an important aluminum detoxification mechanism for various plants; however, how this process is completed remains unclear. In this study, the candidate oxalate transporter gene <i>AtOT</i>, encoding 287 amino acids, was cloned and identified from <i>Arabidopsis thaliana</i>. <i>AtOT</i> was upregulated in response to aluminum stress at the transcriptional level, which was closely related to aluminum treatment concentration and time. The root growth of <i>Arabidopsis</i> was inhibited after knocking out <i>AtOT</i>, and this effect was amplified by aluminum stress. Yeast cells expressing <i>AtOT</i> enhanced oxalic acid resistance and aluminum tolerance, which was closely correlated with the secretion of oxalic acid by membrane vesicle transport. Collectively, these results underline an external exclusion mechanism of oxalate involving <i>AtOT</i> to enhance oxalic acid resistance and aluminum tolerance.https://www.mdpi.com/1422-0067/24/5/4516<i>Arabidopsis thaliana</i>AtOTaluminum toxicityoxalate transportergene expressionfunctional characterization |
spellingShingle | Zongming Yang Pingjuan Zhao Xuehua Luo Wentao Peng Zifan Liu Guishui Xie Mengyue Wang Feng An An Oxalate Transporter Gene, <i>AtOT</i>, Enhances Aluminum Tolerance in <i>Arabidopsis thaliana</i> by Regulating Oxalate Efflux International Journal of Molecular Sciences <i>Arabidopsis thaliana</i> AtOT aluminum toxicity oxalate transporter gene expression functional characterization |
title | An Oxalate Transporter Gene, <i>AtOT</i>, Enhances Aluminum Tolerance in <i>Arabidopsis thaliana</i> by Regulating Oxalate Efflux |
title_full | An Oxalate Transporter Gene, <i>AtOT</i>, Enhances Aluminum Tolerance in <i>Arabidopsis thaliana</i> by Regulating Oxalate Efflux |
title_fullStr | An Oxalate Transporter Gene, <i>AtOT</i>, Enhances Aluminum Tolerance in <i>Arabidopsis thaliana</i> by Regulating Oxalate Efflux |
title_full_unstemmed | An Oxalate Transporter Gene, <i>AtOT</i>, Enhances Aluminum Tolerance in <i>Arabidopsis thaliana</i> by Regulating Oxalate Efflux |
title_short | An Oxalate Transporter Gene, <i>AtOT</i>, Enhances Aluminum Tolerance in <i>Arabidopsis thaliana</i> by Regulating Oxalate Efflux |
title_sort | oxalate transporter gene i atot i enhances aluminum tolerance in i arabidopsis thaliana i by regulating oxalate efflux |
topic | <i>Arabidopsis thaliana</i> AtOT aluminum toxicity oxalate transporter gene expression functional characterization |
url | https://www.mdpi.com/1422-0067/24/5/4516 |
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