In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.)
Objective: To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe (OP) grade black tea with a view to develop an anti-aging skin formulation. Methods: Five concentrations (0.125, 0.250, 0.500, 1.000 and 2.000 mg/mL) of black tea brew (BTB) were made using a freeze dr...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-12-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115301118 |
| _version_ | 1818290622929305600 |
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| author | Wanigasekera Daya Ratnasooriya Walimuni Prabhashini Kaushalya Mendis Abeysekera Chatura Tissa Dayendra Ratnasooriya |
| author_facet | Wanigasekera Daya Ratnasooriya Walimuni Prabhashini Kaushalya Mendis Abeysekera Chatura Tissa Dayendra Ratnasooriya |
| author_sort | Wanigasekera Daya Ratnasooriya |
| collection | DOAJ |
| description | Objective: To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe (OP) grade black tea with a view to develop an anti-aging skin formulation.
Methods: Five concentrations (0.125, 0.250, 0.500, 1.000 and 2.000 mg/mL) of black tea brew (BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification. Epigallocatechin gallate (EGCG) was used as the reference agent (concentrations tested: 0.012, 0.025, 0.050, 0.100 and 0.200 mg/mL). Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid (from rooster comb) and bovine testicular hyaluronidase.
Results: The results revealed that BTB had moderate [IC50=(1.09±0.12) mg/mL] and dose dependent (r2=0.94) anti-hyaluronidase activity. EGCG also exhibited dose dependent (r2=0.93, P<0.05) anti-hyaluronidase activity which was superior [IC50=(0.09±0.00) mg/mL] to BTB.
Conclusions: Sri Lankan low grown orthodox OP grade black tea has promising anti-hyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical. In addition, it may prove useful as a beverage in the management of allergy, some joint diseases and envenomation. |
| first_indexed | 2024-12-13T02:31:06Z |
| format | Article |
| id | doaj.art-3a009af644e6432cacc4eea2838e7148 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-13T02:31:06Z |
| publishDate | 2014-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-3a009af644e6432cacc4eea2838e71482022-12-22T00:02:30ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912014-12-0141295996310.12980/APJTB.4.2014APJTB-2014-0462In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.)Wanigasekera Daya Ratnasooriya0Walimuni Prabhashini Kaushalya Mendis Abeysekera1Chatura Tissa Dayendra Ratnasooriya2Faculty of Allied Health Sciences, General Sir John Kothalawala Defence University, Ratmalana, 10390, Sri LankaHerbal Technology Section, Industrial Technology Institute, 363, Bauddhaloka Mawatha, Colombo 07, Sri LankaFaculty of Medicine, University of Colombo, Colombo-08, Sri LankaObjective: To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe (OP) grade black tea with a view to develop an anti-aging skin formulation. Methods: Five concentrations (0.125, 0.250, 0.500, 1.000 and 2.000 mg/mL) of black tea brew (BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification. Epigallocatechin gallate (EGCG) was used as the reference agent (concentrations tested: 0.012, 0.025, 0.050, 0.100 and 0.200 mg/mL). Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid (from rooster comb) and bovine testicular hyaluronidase. Results: The results revealed that BTB had moderate [IC50=(1.09±0.12) mg/mL] and dose dependent (r2=0.94) anti-hyaluronidase activity. EGCG also exhibited dose dependent (r2=0.93, P<0.05) anti-hyaluronidase activity which was superior [IC50=(0.09±0.00) mg/mL] to BTB. Conclusions: Sri Lankan low grown orthodox OP grade black tea has promising anti-hyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical. In addition, it may prove useful as a beverage in the management of allergy, some joint diseases and envenomation.http://www.sciencedirect.com/science/article/pii/S2221169115301118Camellia sinensisOrange pekoeBlack teaSri Lankan teaAnti-agingAnti-hyaluronidaseHyaluronic acid |
| spellingShingle | Wanigasekera Daya Ratnasooriya Walimuni Prabhashini Kaushalya Mendis Abeysekera Chatura Tissa Dayendra Ratnasooriya In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) Asian Pacific Journal of Tropical Biomedicine Camellia sinensis Orange pekoe Black tea Sri Lankan tea Anti-aging Anti-hyaluronidase Hyaluronic acid |
| title | In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) |
| title_full | In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) |
| title_fullStr | In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) |
| title_full_unstemmed | In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) |
| title_short | In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) |
| title_sort | in vitro anti hyaluronidase activity of sri lankan low grown orthodox orange pekoe grade black tea camellia sinensis l |
| topic | Camellia sinensis Orange pekoe Black tea Sri Lankan tea Anti-aging Anti-hyaluronidase Hyaluronic acid |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115301118 |
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