Determination of mercury concentration by a new spectrophotometric method and evaluation of bacterial diversity in river water samples from Brazil
One of the main micropollutants reported in river water is mercury (Hg), a heavy metal toxic to human and animal organisms that can promote bacterial resistance to antimicrobials used in the clinical practice. Having done this in consideration, monitoring the concentration of Hg in the river is an i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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IWA Publishing
2022-05-01
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| Series: | Water Supply |
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| Online Access: | http://ws.iwaponline.com/content/22/5/5535 |
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| author | Daniela Carolina Simião Frank Pereira de Andrade William Gustavo Lima Maria Larissa de Jesus Pedro Henrique Gomes Dorim Magna Cristina Paiva |
| author_facet | Daniela Carolina Simião Frank Pereira de Andrade William Gustavo Lima Maria Larissa de Jesus Pedro Henrique Gomes Dorim Magna Cristina Paiva |
| author_sort | Daniela Carolina Simião |
| collection | DOAJ |
| description | One of the main micropollutants reported in river water is mercury (Hg), a heavy metal toxic to human and animal organisms that can promote bacterial resistance to antimicrobials used in the clinical practice. Having done this in consideration, monitoring the concentration of Hg in the river is an important indicator of physical-chemical and microbiological quality of water. Thus, in this study, the Hg concentration was determined using a new spectrophotometric method in river water samples recovered from Minas Gerais, Brazil. Furthermore, the diversity and antimicrobial resistance of Gram-positive and Gram-negative bacteria isolated from these samples were also reported. A new ultraviolet-visible spectrophotometric method was validated and applied to quantify Hg in water and revealed high concentrations in the samples (0.13–0.35 μg·mL−1), above the limits established by Brazilian standards (0.002 μg·mL−1). Gram-negative bacteria (mainly Escherichia coli) were recovered in greater proportion (88.4%) from water samples with high mercury concentration and showed resistance to tetracycline and ampicillin. Our results highlighted that E. coli resistant to carbapenems, which are latest generation beta-lactams, were detected. In conclusion, the Hg levels are considerably high in river waters in Brazil, and these mercury-rich water sources are important reservoirs of multi-drug resistant bacteria. HIGHLIGHTS
New spectrophotometry method was validated and applied to determine the mercury concentration in river water samples.;
Mercury concentrations above the limits established by Brazilian standards was reported in river from Minas Gerais.;
Resistance to antimicrobials was detected in bacteria isolates from mercury-rich river water sample.;
Carbapenem-resistant Escherichia coli were reported in mercury-rich river water sample.; |
| first_indexed | 2024-12-10T17:16:42Z |
| format | Article |
| id | doaj.art-3a028935b4714d92866b24c7296d9d07 |
| institution | Directory Open Access Journal |
| issn | 1606-9749 1607-0798 |
| language | English |
| last_indexed | 2024-12-10T17:16:42Z |
| publishDate | 2022-05-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Water Supply |
| spelling | doaj.art-3a028935b4714d92866b24c7296d9d072022-12-22T01:40:06ZengIWA PublishingWater Supply1606-97491607-07982022-05-012255535554810.2166/ws.2022.173173Determination of mercury concentration by a new spectrophotometric method and evaluation of bacterial diversity in river water samples from BrazilDaniela Carolina Simião0Frank Pereira de Andrade1William Gustavo Lima2Maria Larissa de Jesus3Pedro Henrique Gomes Dorim4Magna Cristina Paiva5 Laboratório de Química Fundamental e Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del Rei, Campus Centro Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil Laboratório de Química Fundamental e Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del Rei, Campus Centro Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del Rei, Campus Centro Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil Laboratório de Química Fundamental e Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del Rei, Campus Centro Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil Laboratório de Química Fundamental e Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del Rei, Campus Centro Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del Rei, Campus Centro Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil One of the main micropollutants reported in river water is mercury (Hg), a heavy metal toxic to human and animal organisms that can promote bacterial resistance to antimicrobials used in the clinical practice. Having done this in consideration, monitoring the concentration of Hg in the river is an important indicator of physical-chemical and microbiological quality of water. Thus, in this study, the Hg concentration was determined using a new spectrophotometric method in river water samples recovered from Minas Gerais, Brazil. Furthermore, the diversity and antimicrobial resistance of Gram-positive and Gram-negative bacteria isolated from these samples were also reported. A new ultraviolet-visible spectrophotometric method was validated and applied to quantify Hg in water and revealed high concentrations in the samples (0.13–0.35 μg·mL−1), above the limits established by Brazilian standards (0.002 μg·mL−1). Gram-negative bacteria (mainly Escherichia coli) were recovered in greater proportion (88.4%) from water samples with high mercury concentration and showed resistance to tetracycline and ampicillin. Our results highlighted that E. coli resistant to carbapenems, which are latest generation beta-lactams, were detected. In conclusion, the Hg levels are considerably high in river waters in Brazil, and these mercury-rich water sources are important reservoirs of multi-drug resistant bacteria. HIGHLIGHTS New spectrophotometry method was validated and applied to determine the mercury concentration in river water samples.; Mercury concentrations above the limits established by Brazilian standards was reported in river from Minas Gerais.; Resistance to antimicrobials was detected in bacteria isolates from mercury-rich river water sample.; Carbapenem-resistant Escherichia coli were reported in mercury-rich river water sample.;http://ws.iwaponline.com/content/22/5/5535ampc betalactamaseextended-spectrum betalactamase (esbl)klebsiella pneumoniae carbapenemase (kpc)mercuryriversusceptibility profile |
| spellingShingle | Daniela Carolina Simião Frank Pereira de Andrade William Gustavo Lima Maria Larissa de Jesus Pedro Henrique Gomes Dorim Magna Cristina Paiva Determination of mercury concentration by a new spectrophotometric method and evaluation of bacterial diversity in river water samples from Brazil Water Supply ampc betalactamase extended-spectrum betalactamase (esbl) klebsiella pneumoniae carbapenemase (kpc) mercury river susceptibility profile |
| title | Determination of mercury concentration by a new spectrophotometric method and evaluation of bacterial diversity in river water samples from Brazil |
| title_full | Determination of mercury concentration by a new spectrophotometric method and evaluation of bacterial diversity in river water samples from Brazil |
| title_fullStr | Determination of mercury concentration by a new spectrophotometric method and evaluation of bacterial diversity in river water samples from Brazil |
| title_full_unstemmed | Determination of mercury concentration by a new spectrophotometric method and evaluation of bacterial diversity in river water samples from Brazil |
| title_short | Determination of mercury concentration by a new spectrophotometric method and evaluation of bacterial diversity in river water samples from Brazil |
| title_sort | determination of mercury concentration by a new spectrophotometric method and evaluation of bacterial diversity in river water samples from brazil |
| topic | ampc betalactamase extended-spectrum betalactamase (esbl) klebsiella pneumoniae carbapenemase (kpc) mercury river susceptibility profile |
| url | http://ws.iwaponline.com/content/22/5/5535 |
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