POLYMERASE CHAIN REACTION AND FLUORESCENT IN SITU HYBRIDIZATION USED FOR BOVINE EMBRYO SEXING
The purpose of this paper was to evaluate the results obtained for bovine embryo sexing using the polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) and to compare them in order to decide which of the two methods is more accurate and yields better results while neces...
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| Format: | Article |
| Language: | English |
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University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
2009-03-01
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| Series: | Cluj Veterinary Journal |
| Subjects: | |
| Online Access: | https://clujveterinaryjournal.ro/index.php/cvj/article/view/1 |
| _version_ | 1811268408421908480 |
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| author | Mihai CENARIU Ioan GROZA Liviu Bogdan Emoke PALL Simona CIUPE |
| author_facet | Mihai CENARIU Ioan GROZA Liviu Bogdan Emoke PALL Simona CIUPE |
| author_sort | Mihai CENARIU |
| collection | DOAJ |
| description |
The purpose of this paper was to evaluate the results obtained for bovine embryo sexing using the polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) and to compare them in order to decide which of the two methods is more accurate and yields better results while necessitating less effort. We took into consideration the pregnancy rate obtained after the transfer of biopsied embryos, the percentage of correctly sexed embryos evaluated at birth (when the predicted sex was compared with the actual sex of the newborn) as well as other characteristics related to the difficulty of the method, expenses and suitability to a minimally equipped laboratory. We concluded that the polymerase chain reaction is the most accurate and suitable method for sexing preimplantation bovine embryos, being in the same time easier to perform than the fluorescence in situ hybridization.
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| first_indexed | 2024-04-12T21:21:17Z |
| format | Article |
| id | doaj.art-3a047f7b9b24414684bc8efb0f026223 |
| institution | Directory Open Access Journal |
| issn | 2066-9399 |
| language | English |
| last_indexed | 2024-04-12T21:21:17Z |
| publishDate | 2009-03-01 |
| publisher | University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca |
| record_format | Article |
| series | Cluj Veterinary Journal |
| spelling | doaj.art-3a047f7b9b24414684bc8efb0f0262232022-12-22T03:16:17ZengUniversity of Agricultural Sciences and Veterinary Medicine Cluj-NapocaCluj Veterinary Journal2066-93992009-03-0115110.52331/cvj.v15i1.1POLYMERASE CHAIN REACTION AND FLUORESCENT IN SITU HYBRIDIZATION USED FOR BOVINE EMBRYO SEXINGMihai CENARIU0Ioan GROZA1Liviu Bogdan2Emoke PALL3Simona CIUPE4University of Agricultural Sciences and Veterinary MedicineUniversity of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Department of Veterinary Reproduction, Obstetrics and GynecologyUniversity of Agricultural Sciences and Veterinary MedicineUniversity of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Department of Veterinary Reproduction, Obstetrics and GynecologyUniversity of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Department of Veterinary Reproduction, Obstetrics and Gynecology The purpose of this paper was to evaluate the results obtained for bovine embryo sexing using the polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) and to compare them in order to decide which of the two methods is more accurate and yields better results while necessitating less effort. We took into consideration the pregnancy rate obtained after the transfer of biopsied embryos, the percentage of correctly sexed embryos evaluated at birth (when the predicted sex was compared with the actual sex of the newborn) as well as other characteristics related to the difficulty of the method, expenses and suitability to a minimally equipped laboratory. We concluded that the polymerase chain reaction is the most accurate and suitable method for sexing preimplantation bovine embryos, being in the same time easier to perform than the fluorescence in situ hybridization. https://clujveterinaryjournal.ro/index.php/cvj/article/view/1bovine embryobiopsypolymerase chain reactionfluorescent in situ hybridization |
| spellingShingle | Mihai CENARIU Ioan GROZA Liviu Bogdan Emoke PALL Simona CIUPE POLYMERASE CHAIN REACTION AND FLUORESCENT IN SITU HYBRIDIZATION USED FOR BOVINE EMBRYO SEXING Cluj Veterinary Journal bovine embryo biopsy polymerase chain reaction fluorescent in situ hybridization |
| title | POLYMERASE CHAIN REACTION AND FLUORESCENT IN SITU HYBRIDIZATION USED FOR BOVINE EMBRYO SEXING |
| title_full | POLYMERASE CHAIN REACTION AND FLUORESCENT IN SITU HYBRIDIZATION USED FOR BOVINE EMBRYO SEXING |
| title_fullStr | POLYMERASE CHAIN REACTION AND FLUORESCENT IN SITU HYBRIDIZATION USED FOR BOVINE EMBRYO SEXING |
| title_full_unstemmed | POLYMERASE CHAIN REACTION AND FLUORESCENT IN SITU HYBRIDIZATION USED FOR BOVINE EMBRYO SEXING |
| title_short | POLYMERASE CHAIN REACTION AND FLUORESCENT IN SITU HYBRIDIZATION USED FOR BOVINE EMBRYO SEXING |
| title_sort | polymerase chain reaction and fluorescent in situ hybridization used for bovine embryo sexing |
| topic | bovine embryo biopsy polymerase chain reaction fluorescent in situ hybridization |
| url | https://clujveterinaryjournal.ro/index.php/cvj/article/view/1 |
| work_keys_str_mv | AT mihaicenariu polymerasechainreactionandfluorescentinsituhybridizationusedforbovineembryosexing AT ioangroza polymerasechainreactionandfluorescentinsituhybridizationusedforbovineembryosexing AT liviubogdan polymerasechainreactionandfluorescentinsituhybridizationusedforbovineembryosexing AT emokepall polymerasechainreactionandfluorescentinsituhybridizationusedforbovineembryosexing AT simonaciupe polymerasechainreactionandfluorescentinsituhybridizationusedforbovineembryosexing |