Polish Yellow Sweet Clover (Melilotus officinalis L.) Honey, Chromatographic Fingerprints, and Chemical Markers
A case study of Polish Melilotus officinalis honey was presented for the first time. Gas chromatography–mass spectrometry (GC-MS) (after steam distillation, Soxhlet extraction, ultrasonic solvent extraction, and solid phase extraction (SPE)) and targeted high performance liquid chromatography with a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/22/1/138 |
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| author | Izabela Jasicka-Misiak Ewa Makowicz Natalia Stanek |
| author_facet | Izabela Jasicka-Misiak Ewa Makowicz Natalia Stanek |
| author_sort | Izabela Jasicka-Misiak |
| collection | DOAJ |
| description | A case study of Polish Melilotus officinalis honey was presented for the first time. Gas chromatography–mass spectrometry (GC-MS) (after steam distillation, Soxhlet extraction, ultrasonic solvent extraction, and solid phase extraction (SPE)) and targeted high performance liquid chromatography with a photodiode array detector (HPLC-PAD) were applied to determine the characteristic components of honey. While ubiquitous in most honeys, carbohydrates, terpene derivatives, and phenylacetic acid dominated in the Soxhlet extracts (25.54%) and in the application of SPE (13.04%). In addition, lumichrome (1.85%) was found, and may be considered as a marker of this honey. Due to the presence of these compounds, Polish yellow sweet clover honey is similar to French lavender honeys. The major compounds determined in the methanolic extract were (+)-catechine (39.7%) and gallic acid (up to 30%), which can be regarded as specific chemical markers of the botanical origin of melilot honey. With respect to total phenolic and flavonoid contents, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were determined spectrophotometrically. The honey exhibited a moderate antioxidant activity, typical for light honeys, which correlates well with its phenolic and flavonoid composition. |
| first_indexed | 2024-12-20T20:38:04Z |
| format | Article |
| id | doaj.art-3a0909b6f027446fb9331ef7101d4371 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-20T20:38:04Z |
| publishDate | 2017-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3a0909b6f027446fb9331ef7101d43712022-12-21T19:27:10ZengMDPI AGMolecules1420-30492017-01-0122113810.3390/molecules22010138molecules22010138Polish Yellow Sweet Clover (Melilotus officinalis L.) Honey, Chromatographic Fingerprints, and Chemical MarkersIzabela Jasicka-Misiak0Ewa Makowicz1Natalia Stanek2Faculty of Chemistry, Opole University, Oleska 48, 45-052 Opole, PolandFaculty of Chemistry, Opole University, Oleska 48, 45-052 Opole, PolandFaculty of Chemistry, Opole University, Oleska 48, 45-052 Opole, PolandA case study of Polish Melilotus officinalis honey was presented for the first time. Gas chromatography–mass spectrometry (GC-MS) (after steam distillation, Soxhlet extraction, ultrasonic solvent extraction, and solid phase extraction (SPE)) and targeted high performance liquid chromatography with a photodiode array detector (HPLC-PAD) were applied to determine the characteristic components of honey. While ubiquitous in most honeys, carbohydrates, terpene derivatives, and phenylacetic acid dominated in the Soxhlet extracts (25.54%) and in the application of SPE (13.04%). In addition, lumichrome (1.85%) was found, and may be considered as a marker of this honey. Due to the presence of these compounds, Polish yellow sweet clover honey is similar to French lavender honeys. The major compounds determined in the methanolic extract were (+)-catechine (39.7%) and gallic acid (up to 30%), which can be regarded as specific chemical markers of the botanical origin of melilot honey. With respect to total phenolic and flavonoid contents, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were determined spectrophotometrically. The honey exhibited a moderate antioxidant activity, typical for light honeys, which correlates well with its phenolic and flavonoid composition.http://www.mdpi.com/1420-3049/22/1/138yellow sweet cloverMelilotus officinalisphenolicvolatilehoney markers |
| spellingShingle | Izabela Jasicka-Misiak Ewa Makowicz Natalia Stanek Polish Yellow Sweet Clover (Melilotus officinalis L.) Honey, Chromatographic Fingerprints, and Chemical Markers Molecules yellow sweet clover Melilotus officinalis phenolic volatile honey markers |
| title | Polish Yellow Sweet Clover (Melilotus officinalis L.) Honey, Chromatographic Fingerprints, and Chemical Markers |
| title_full | Polish Yellow Sweet Clover (Melilotus officinalis L.) Honey, Chromatographic Fingerprints, and Chemical Markers |
| title_fullStr | Polish Yellow Sweet Clover (Melilotus officinalis L.) Honey, Chromatographic Fingerprints, and Chemical Markers |
| title_full_unstemmed | Polish Yellow Sweet Clover (Melilotus officinalis L.) Honey, Chromatographic Fingerprints, and Chemical Markers |
| title_short | Polish Yellow Sweet Clover (Melilotus officinalis L.) Honey, Chromatographic Fingerprints, and Chemical Markers |
| title_sort | polish yellow sweet clover melilotus officinalis l honey chromatographic fingerprints and chemical markers |
| topic | yellow sweet clover Melilotus officinalis phenolic volatile honey markers |
| url | http://www.mdpi.com/1420-3049/22/1/138 |
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