A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep
The development of novel biodegradable vascular grafts of a small diameter (<6 mm) is an unmet clinical need for patients requiring arterial replacement. Here we performed a pre-clinical study of new small-caliber biodegradable vascular grafts using a sheep model of carotid artery implantation. T...
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MDPI AG
2020-05-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/13/5/101 |
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| author | Larisa V. Antonova Andrey V. Mironov Arseniy E. Yuzhalin Evgeniya O. Krivkina Amin R. Shabaev Maria A. Rezvova Vadim O. Tkachenko Mariam Yu. Khanova Tatiana Yu. Sergeeva Sergei S. Krutitskiy Leonid S. Barbarash |
| author_facet | Larisa V. Antonova Andrey V. Mironov Arseniy E. Yuzhalin Evgeniya O. Krivkina Amin R. Shabaev Maria A. Rezvova Vadim O. Tkachenko Mariam Yu. Khanova Tatiana Yu. Sergeeva Sergei S. Krutitskiy Leonid S. Barbarash |
| author_sort | Larisa V. Antonova |
| collection | DOAJ |
| description | The development of novel biodegradable vascular grafts of a small diameter (<6 mm) is an unmet clinical need for patients requiring arterial replacement. Here we performed a pre-clinical study of new small-caliber biodegradable vascular grafts using a sheep model of carotid artery implantation. The 4 mm diameter vascular grafts were manufactured using a mix of polyhydroxybutyrate/valerate and polycaprolactone supplemented with growth factors VEGF, bFGF and SDF-1α (PHBV/PCL-GFmix) and additionally modified by a polymer hydrogel coating with incorporation of drugs heparin and iloprost (PHBV/PCL-GFmix<sup>Hep/Ilo</sup>). Animals with carotid artery autograft implantation and those implanted with clinically used GORE-TEX<sup>®</sup> grafts were used as control groups. We observed that 24 h following surgery, animals with carotid artery autograft implantation showed 87.5% patency, while all the PHBV/PCL-GFmix and GORE-TEX<sup>®</sup> grafts displayed thrombosis. PHBV/PCL-GFmix<sup>Hep/Ilo</sup> grafts demonstrated 62.5% patency 24 h following surgery and it had remained at 50% 1 year post-operation. All the PHBV/PCL grafts completely degraded less than 1 year following surgery and were replaced by de novo vasculature without evidence of calcification. On the other hand, GORE-TEX<sup>®</sup> grafts displayed substantial amounts of calcium deposits throughout graft tissues. Thus, here we report a potential clinical usefulness of PHBV/PCL grafts upon their additional modification by growth factors and drugs to promote endothelialization and reduce thrombogenicity. |
| first_indexed | 2024-03-10T19:40:31Z |
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| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T19:40:31Z |
| publishDate | 2020-05-01 |
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| spelling | doaj.art-3a093fc21f2a455cbead99d0de42d05f2023-11-20T01:16:56ZengMDPI AGPharmaceuticals1424-82472020-05-0113510110.3390/ph13050101A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the SheepLarisa V. Antonova0Andrey V. Mironov1Arseniy E. Yuzhalin2Evgeniya O. Krivkina3Amin R. Shabaev4Maria A. Rezvova5Vadim O. Tkachenko6Mariam Yu. Khanova7Tatiana Yu. Sergeeva8Sergei S. Krutitskiy9Leonid S. Barbarash10Research Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Blvd, Kemerovo 650002, RussiaResearch Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Blvd, Kemerovo 650002, RussiaResearch Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Blvd, Kemerovo 650002, RussiaResearch Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Blvd, Kemerovo 650002, RussiaResearch Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Blvd, Kemerovo 650002, RussiaResearch Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Blvd, Kemerovo 650002, RussiaBudker Institute of Nuclear Physics SB RAS, 11 akademika Lavrentieva Ave, Novosibirsk 630090, RussiaResearch Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Blvd, Kemerovo 650002, RussiaResearch Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Blvd, Kemerovo 650002, RussiaResearch Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Blvd, Kemerovo 650002, RussiaResearch Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Blvd, Kemerovo 650002, RussiaThe development of novel biodegradable vascular grafts of a small diameter (<6 mm) is an unmet clinical need for patients requiring arterial replacement. Here we performed a pre-clinical study of new small-caliber biodegradable vascular grafts using a sheep model of carotid artery implantation. The 4 mm diameter vascular grafts were manufactured using a mix of polyhydroxybutyrate/valerate and polycaprolactone supplemented with growth factors VEGF, bFGF and SDF-1α (PHBV/PCL-GFmix) and additionally modified by a polymer hydrogel coating with incorporation of drugs heparin and iloprost (PHBV/PCL-GFmix<sup>Hep/Ilo</sup>). Animals with carotid artery autograft implantation and those implanted with clinically used GORE-TEX<sup>®</sup> grafts were used as control groups. We observed that 24 h following surgery, animals with carotid artery autograft implantation showed 87.5% patency, while all the PHBV/PCL-GFmix and GORE-TEX<sup>®</sup> grafts displayed thrombosis. PHBV/PCL-GFmix<sup>Hep/Ilo</sup> grafts demonstrated 62.5% patency 24 h following surgery and it had remained at 50% 1 year post-operation. All the PHBV/PCL grafts completely degraded less than 1 year following surgery and were replaced by de novo vasculature without evidence of calcification. On the other hand, GORE-TEX<sup>®</sup> grafts displayed substantial amounts of calcium deposits throughout graft tissues. Thus, here we report a potential clinical usefulness of PHBV/PCL grafts upon their additional modification by growth factors and drugs to promote endothelialization and reduce thrombogenicity.https://www.mdpi.com/1424-8247/13/5/101biodegradable vascular graftpolycaprolactonecarotid artery implantationsheeppre-clinical studyheparin |
| spellingShingle | Larisa V. Antonova Andrey V. Mironov Arseniy E. Yuzhalin Evgeniya O. Krivkina Amin R. Shabaev Maria A. Rezvova Vadim O. Tkachenko Mariam Yu. Khanova Tatiana Yu. Sergeeva Sergei S. Krutitskiy Leonid S. Barbarash A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep Pharmaceuticals biodegradable vascular graft polycaprolactone carotid artery implantation sheep pre-clinical study heparin |
| title | A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep |
| title_full | A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep |
| title_fullStr | A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep |
| title_full_unstemmed | A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep |
| title_short | A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep |
| title_sort | brief report on an implantation of small caliber biodegradable vascular grafts in a carotid artery of the sheep |
| topic | biodegradable vascular graft polycaprolactone carotid artery implantation sheep pre-clinical study heparin |
| url | https://www.mdpi.com/1424-8247/13/5/101 |
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