Cellulase Enzyme Production from Filamentous Fungi <i>Trichoderma reesei</i> and <i>Aspergillus awamori</i> in Submerged Fermentation with Rice Straw
Fungi are a diverse group of microorganisms that play many roles in human livelihoods. However, the isolation of potential fungal species is the key factor to their utilization in different sectors, including the enzyme industry. Hence, in this study, we used two different fungal repositories—soil a...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/10/868 |
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| author | Laila Naher Siti Noor Fatin Md Abdul Halim Sheikh Lateef Adebola Azeez Shaiquzzaman Siddiquee Norhafizah Md Zain Sarker Mohammad Rezaul Karim |
| author_facet | Laila Naher Siti Noor Fatin Md Abdul Halim Sheikh Lateef Adebola Azeez Shaiquzzaman Siddiquee Norhafizah Md Zain Sarker Mohammad Rezaul Karim |
| author_sort | Laila Naher |
| collection | DOAJ |
| description | Fungi are a diverse group of microorganisms that play many roles in human livelihoods. However, the isolation of potential fungal species is the key factor to their utilization in different sectors, including the enzyme industry. Hence, in this study, we used two different fungal repositories—soil and weed leaves—to isolate filamentous fungi and evaluate their potential to produce the cellulase enzyme. The fungal strains were isolated using dichloran rose bengal agar (DRBA) and potato dextrose agar (PDA). For cellulase enzyme production, a rice straw submerged fermentation process was used. The enzyme production was carried out at the different incubation times of 3, 5, and 7 days of culture in submerged conditions with rice straw. Fungal identification studies by morphological and molecular methods showed that the soil colonies matched with <i>Trichoderma reesei,</i> and the weed leaf colonies matched with <i>Aspergillus awamori</i>. These species were coded as <i>T. reesei</i> UMK04 and <i>A. awamori</i> UMK02, respectively. This is the first report of <i>A. awamori</i> UMK02 isolation in Malaysian agriculture. The results of cellulase production using the two fungi incorporated with rice straw submerged fermentation showed that <i>T. reesei</i> produced a higher amount of cellulase at Day 5 (27.04 U/mg of dry weight) as compared with <i>A. awamori</i> (15.19 U/mg of dry weight), and the concentration was significantly different (<i>p</i> < 0.05). Our results imply that <i>T. reesei</i> can be utilized for cellulase production using rice straw. |
| first_indexed | 2024-03-10T06:27:43Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T06:27:43Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-3a09eadaa5d7443a9b9f965eb2b4be472023-11-22T18:47:46ZengMDPI AGJournal of Fungi2309-608X2021-10-0171086810.3390/jof7100868Cellulase Enzyme Production from Filamentous Fungi <i>Trichoderma reesei</i> and <i>Aspergillus awamori</i> in Submerged Fermentation with Rice StrawLaila Naher0Siti Noor Fatin1Md Abdul Halim Sheikh2Lateef Adebola Azeez3Shaiquzzaman Siddiquee4Norhafizah Md Zain5Sarker Mohammad Rezaul Karim6Faculty of Agro-Based Industry, Universiti Malaysia Kelantan Jeli Campus, Jeli 17600, MalaysiaFaculty of Agro-Based Industry, Universiti Malaysia Kelantan Jeli Campus, Jeli 17600, MalaysiaFaculty of Agro-Based Industry, Universiti Malaysia Kelantan Jeli Campus, Jeli 17600, MalaysiaFaculty of Agro-Based Industry, Universiti Malaysia Kelantan Jeli Campus, Jeli 17600, MalaysiaBiotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, MalaysiaFaculty of Agro-Based Industry, Universiti Malaysia Kelantan Jeli Campus, Jeli 17600, MalaysiaFaculty of Agriculture, Universiti Putra Malaysia, UPM, Serdang 43400, MalaysiaFungi are a diverse group of microorganisms that play many roles in human livelihoods. However, the isolation of potential fungal species is the key factor to their utilization in different sectors, including the enzyme industry. Hence, in this study, we used two different fungal repositories—soil and weed leaves—to isolate filamentous fungi and evaluate their potential to produce the cellulase enzyme. The fungal strains were isolated using dichloran rose bengal agar (DRBA) and potato dextrose agar (PDA). For cellulase enzyme production, a rice straw submerged fermentation process was used. The enzyme production was carried out at the different incubation times of 3, 5, and 7 days of culture in submerged conditions with rice straw. Fungal identification studies by morphological and molecular methods showed that the soil colonies matched with <i>Trichoderma reesei,</i> and the weed leaf colonies matched with <i>Aspergillus awamori</i>. These species were coded as <i>T. reesei</i> UMK04 and <i>A. awamori</i> UMK02, respectively. This is the first report of <i>A. awamori</i> UMK02 isolation in Malaysian agriculture. The results of cellulase production using the two fungi incorporated with rice straw submerged fermentation showed that <i>T. reesei</i> produced a higher amount of cellulase at Day 5 (27.04 U/mg of dry weight) as compared with <i>A. awamori</i> (15.19 U/mg of dry weight), and the concentration was significantly different (<i>p</i> < 0.05). Our results imply that <i>T. reesei</i> can be utilized for cellulase production using rice straw.https://www.mdpi.com/2309-608X/7/10/868cellulase enzyme<i>Aspergillus awamori</i><i>Trichoderma reesei</i>rice strawfermentation |
| spellingShingle | Laila Naher Siti Noor Fatin Md Abdul Halim Sheikh Lateef Adebola Azeez Shaiquzzaman Siddiquee Norhafizah Md Zain Sarker Mohammad Rezaul Karim Cellulase Enzyme Production from Filamentous Fungi <i>Trichoderma reesei</i> and <i>Aspergillus awamori</i> in Submerged Fermentation with Rice Straw Journal of Fungi cellulase enzyme <i>Aspergillus awamori</i> <i>Trichoderma reesei</i> rice straw fermentation |
| title | Cellulase Enzyme Production from Filamentous Fungi <i>Trichoderma reesei</i> and <i>Aspergillus awamori</i> in Submerged Fermentation with Rice Straw |
| title_full | Cellulase Enzyme Production from Filamentous Fungi <i>Trichoderma reesei</i> and <i>Aspergillus awamori</i> in Submerged Fermentation with Rice Straw |
| title_fullStr | Cellulase Enzyme Production from Filamentous Fungi <i>Trichoderma reesei</i> and <i>Aspergillus awamori</i> in Submerged Fermentation with Rice Straw |
| title_full_unstemmed | Cellulase Enzyme Production from Filamentous Fungi <i>Trichoderma reesei</i> and <i>Aspergillus awamori</i> in Submerged Fermentation with Rice Straw |
| title_short | Cellulase Enzyme Production from Filamentous Fungi <i>Trichoderma reesei</i> and <i>Aspergillus awamori</i> in Submerged Fermentation with Rice Straw |
| title_sort | cellulase enzyme production from filamentous fungi i trichoderma reesei i and i aspergillus awamori i in submerged fermentation with rice straw |
| topic | cellulase enzyme <i>Aspergillus awamori</i> <i>Trichoderma reesei</i> rice straw fermentation |
| url | https://www.mdpi.com/2309-608X/7/10/868 |
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