Surface Roughness and <i>Streptococcus mutans</i> Adhesion on Metallic and Ceramic Fixed Prosthodontic Materials after Scaling
The aim of this study was to evaluate the surface roughness of fixed prosthodontic materials after polishing or roughening with a stainless steel curette or ultrasonic scaler and to examine the effect of these on <i>Streptococcus mutans</i> adhesion and biofilm accumulation. Thirty speci...
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MDPI AG
2021-02-01
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| Online Access: | https://www.mdpi.com/1996-1944/14/4/1027 |
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| author | Jenni Hjerppe Sampo Rodas Johanna Korvala Paula Pesonen Anna Kaisanlahti Mutlu Özcan Juho Suojanen Justus Reunanen |
| author_facet | Jenni Hjerppe Sampo Rodas Johanna Korvala Paula Pesonen Anna Kaisanlahti Mutlu Özcan Juho Suojanen Justus Reunanen |
| author_sort | Jenni Hjerppe |
| collection | DOAJ |
| description | The aim of this study was to evaluate the surface roughness of fixed prosthodontic materials after polishing or roughening with a stainless steel curette or ultrasonic scaler and to examine the effect of these on <i>Streptococcus mutans</i> adhesion and biofilm accumulation. Thirty specimens (10 × 10 × 3 mm<sup>3</sup>) of zirconia (Zr), pressed lithium disilicate (LDS-Press), milled lithium disilicate glazed (LDS-Glaze), titanium grade V (Ti) and cobalt-chromium (CoCr) were divided into three groups (n = 10) according to surface treatment: polished (C), roughened with stainless steel curette (SC), roughened with ultrasonic scaler (US). Surface roughness values (Sa, Sq) were measured with a spinning disc confocal microscope, and contact angles and surface free energy (SFE) were measured with a contact angle meter. The specimens were covered with sterilized human saliva and immersed into <i>Streptococcus mutans</i> suspensions for bacterial adhesion. The biofilm was allowed to form for 24 h. Sa values were in the range of 0.008–0.139 µm depending on the material and surface treatment. Curette and ultrasonic scaling increased the surface roughness in LDS-Glaze (<i>p</i> < 0.05), Ti (<i>p</i> < 0.01) and CoCr (<i>p</i> < 0.001), however, surface roughness did not affect bacterial adhesion. Zr C and US had a higher bacterial adhesion percentage compared to LDS-Glaze C and US (<i>p</i> = 0.03). There were no differences between study materials in terms of biofilm accumulation. |
| first_indexed | 2024-03-09T00:39:13Z |
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| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T00:39:13Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
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| series | Materials |
| spelling | doaj.art-3a0a1fe008ed448ea00b0716f60d81e92023-12-11T17:57:09ZengMDPI AGMaterials1996-19442021-02-01144102710.3390/ma14041027Surface Roughness and <i>Streptococcus mutans</i> Adhesion on Metallic and Ceramic Fixed Prosthodontic Materials after ScalingJenni Hjerppe0Sampo Rodas1Johanna Korvala2Paula Pesonen3Anna Kaisanlahti4Mutlu Özcan5Juho Suojanen6Justus Reunanen7Clinic of Reconstructive Dentistry, Center of Dental Medicine, University of Zürich, 8032 Zürich, SwitzerlandBiocenter Oulu & Cancer and Translational Medicine Research Unit, University of Oulu, 90014 Oulu, FinlandBiocenter Oulu & Cancer and Translational Medicine Research Unit, University of Oulu, 90014 Oulu, FinlandInfrastructure for Population Studies, Faculty of Medicine, University of Oulu, 90014 Oulu, FinlandBiocenter Oulu & Cancer and Translational Medicine Research Unit, University of Oulu, 90014 Oulu, FinlandCenter of Dental Medicine, Division of Dental Biomaterials, Clinic for Reconstructive Dentistry, University of Zürich, 8032 Zürich, SwitzerlandPäijät-Häme Joint Authority for Health and Wellbeing, Department of Oral and Maxillo-facial Surgery, 15850 Lahti, FinlandBiocenter Oulu & Cancer and Translational Medicine Research Unit, University of Oulu, 90014 Oulu, FinlandThe aim of this study was to evaluate the surface roughness of fixed prosthodontic materials after polishing or roughening with a stainless steel curette or ultrasonic scaler and to examine the effect of these on <i>Streptococcus mutans</i> adhesion and biofilm accumulation. Thirty specimens (10 × 10 × 3 mm<sup>3</sup>) of zirconia (Zr), pressed lithium disilicate (LDS-Press), milled lithium disilicate glazed (LDS-Glaze), titanium grade V (Ti) and cobalt-chromium (CoCr) were divided into three groups (n = 10) according to surface treatment: polished (C), roughened with stainless steel curette (SC), roughened with ultrasonic scaler (US). Surface roughness values (Sa, Sq) were measured with a spinning disc confocal microscope, and contact angles and surface free energy (SFE) were measured with a contact angle meter. The specimens were covered with sterilized human saliva and immersed into <i>Streptococcus mutans</i> suspensions for bacterial adhesion. The biofilm was allowed to form for 24 h. Sa values were in the range of 0.008–0.139 µm depending on the material and surface treatment. Curette and ultrasonic scaling increased the surface roughness in LDS-Glaze (<i>p</i> < 0.05), Ti (<i>p</i> < 0.01) and CoCr (<i>p</i> < 0.001), however, surface roughness did not affect bacterial adhesion. Zr C and US had a higher bacterial adhesion percentage compared to LDS-Glaze C and US (<i>p</i> = 0.03). There were no differences between study materials in terms of biofilm accumulation.https://www.mdpi.com/1996-1944/14/4/1027surface roughnessbacterial adhesionbiofilm<i>Streptococcus mutans</i>zirconialithium disilicate |
| spellingShingle | Jenni Hjerppe Sampo Rodas Johanna Korvala Paula Pesonen Anna Kaisanlahti Mutlu Özcan Juho Suojanen Justus Reunanen Surface Roughness and <i>Streptococcus mutans</i> Adhesion on Metallic and Ceramic Fixed Prosthodontic Materials after Scaling Materials surface roughness bacterial adhesion biofilm <i>Streptococcus mutans</i> zirconia lithium disilicate |
| title | Surface Roughness and <i>Streptococcus mutans</i> Adhesion on Metallic and Ceramic Fixed Prosthodontic Materials after Scaling |
| title_full | Surface Roughness and <i>Streptococcus mutans</i> Adhesion on Metallic and Ceramic Fixed Prosthodontic Materials after Scaling |
| title_fullStr | Surface Roughness and <i>Streptococcus mutans</i> Adhesion on Metallic and Ceramic Fixed Prosthodontic Materials after Scaling |
| title_full_unstemmed | Surface Roughness and <i>Streptococcus mutans</i> Adhesion on Metallic and Ceramic Fixed Prosthodontic Materials after Scaling |
| title_short | Surface Roughness and <i>Streptococcus mutans</i> Adhesion on Metallic and Ceramic Fixed Prosthodontic Materials after Scaling |
| title_sort | surface roughness and i streptococcus mutans i adhesion on metallic and ceramic fixed prosthodontic materials after scaling |
| topic | surface roughness bacterial adhesion biofilm <i>Streptococcus mutans</i> zirconia lithium disilicate |
| url | https://www.mdpi.com/1996-1944/14/4/1027 |
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