An exploratory study of neopterin and kynurenine pathway in pterygium
Pterygium is an inflammatory, vascular and degenerative disorder with unknown aetiology. The aim of this study was to evaluate the changes in neopterin levels, reflecting T-cell immunity, and the kynurenine pathway, the main degradation process of tryptophan, in pterygium. For this purpose, neopteri...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2019-08-01
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| Series: | Pteridines |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/pteridines-2019-0019 |
| _version_ | 1818718313050537984 |
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| author | Kilicarslan Bilge Cardak Aziz Girgin Gozde Kemer Ozlem Evren Baydar Terken |
| author_facet | Kilicarslan Bilge Cardak Aziz Girgin Gozde Kemer Ozlem Evren Baydar Terken |
| author_sort | Kilicarslan Bilge |
| collection | DOAJ |
| description | Pterygium is an inflammatory, vascular and degenerative disorder with unknown aetiology. The aim of this study was to evaluate the changes in neopterin levels, reflecting T-cell immunity, and the kynurenine pathway, the main degradation process of tryptophan, in pterygium. For this purpose, neopterin concentrations were measured in serum and tear samples by enzyme-linked immunosorbent assay (ELISA) in pterygium patients (n=31) and control group (n=32). Kynurenine (KYN) and tryptophan (TRP) serum levels were simultaneously determined by high-performance liquid chromatography (HPLC) for evaluation of the kynurenine pathway. Serum neopterin concentrations and kynurenine to tryptophan ratio (KYN/TRP) as an index of tryptophan breakdown were found increased in pterygium compared to controls (p<0.05). Although there was a 3-fold difference observed between serum and tear neopterin levels, no significant relationship was found. It can be concluded that neopterin may be used as a nonspecific biomarker that reflects immunological activity in pterygium and has clinical potential for evaluation of pterygium pathogenesis. These immune- or inflammatory-mediated changes were also supported by an increased KYN/TRP ratio in pterygium patients. |
| first_indexed | 2024-12-17T19:49:03Z |
| format | Article |
| id | doaj.art-3a0f0ca0b88e4b89a6f0833960daed0f |
| institution | Directory Open Access Journal |
| issn | 0933-4807 2195-4720 |
| language | English |
| last_indexed | 2024-12-17T19:49:03Z |
| publishDate | 2019-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Pteridines |
| spelling | doaj.art-3a0f0ca0b88e4b89a6f0833960daed0f2022-12-21T21:34:46ZengDe GruyterPteridines0933-48072195-47202019-08-0130115315710.1515/pteridines-2019-0019pteridines-2019-0019An exploratory study of neopterin and kynurenine pathway in pterygiumKilicarslan Bilge0Cardak Aziz1Girgin Gozde2Kemer Ozlem Evren3Baydar Terken4Department of Toxicology, Faculty of Pharmacy, Hacettepe University, 90-06100, Ankara, TurkeyOphthalmology Clinic, Numune Research and Education Hospital, 90-06230, Ankara, TurkeyDepartment of Toxicology, Faculty of Pharmacy, Hacettepe University, 90-06100, Ankara, TurkeyOphthalmology Clinic, Numune Research and Education Hospital, 90-06230, Ankara, TurkeyDepartment of Toxicology, Faculty of Pharmacy, Hacettepe University, 90-06100, Ankara, TurkeyPterygium is an inflammatory, vascular and degenerative disorder with unknown aetiology. The aim of this study was to evaluate the changes in neopterin levels, reflecting T-cell immunity, and the kynurenine pathway, the main degradation process of tryptophan, in pterygium. For this purpose, neopterin concentrations were measured in serum and tear samples by enzyme-linked immunosorbent assay (ELISA) in pterygium patients (n=31) and control group (n=32). Kynurenine (KYN) and tryptophan (TRP) serum levels were simultaneously determined by high-performance liquid chromatography (HPLC) for evaluation of the kynurenine pathway. Serum neopterin concentrations and kynurenine to tryptophan ratio (KYN/TRP) as an index of tryptophan breakdown were found increased in pterygium compared to controls (p<0.05). Although there was a 3-fold difference observed between serum and tear neopterin levels, no significant relationship was found. It can be concluded that neopterin may be used as a nonspecific biomarker that reflects immunological activity in pterygium and has clinical potential for evaluation of pterygium pathogenesis. These immune- or inflammatory-mediated changes were also supported by an increased KYN/TRP ratio in pterygium patients.https://doi.org/10.1515/pteridines-2019-0019ocular pathologytryptophantearindoleamine 2,3-dioxygenaseeye disease |
| spellingShingle | Kilicarslan Bilge Cardak Aziz Girgin Gozde Kemer Ozlem Evren Baydar Terken An exploratory study of neopterin and kynurenine pathway in pterygium Pteridines ocular pathology tryptophan tear indoleamine 2,3-dioxygenase eye disease |
| title | An exploratory study of neopterin and kynurenine pathway in pterygium |
| title_full | An exploratory study of neopterin and kynurenine pathway in pterygium |
| title_fullStr | An exploratory study of neopterin and kynurenine pathway in pterygium |
| title_full_unstemmed | An exploratory study of neopterin and kynurenine pathway in pterygium |
| title_short | An exploratory study of neopterin and kynurenine pathway in pterygium |
| title_sort | exploratory study of neopterin and kynurenine pathway in pterygium |
| topic | ocular pathology tryptophan tear indoleamine 2,3-dioxygenase eye disease |
| url | https://doi.org/10.1515/pteridines-2019-0019 |
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