Early reduction of microglia activation by irradiation in a model of chronic glaucoma.
Glaucoma is a neurodegenerative disease that results in the progressive decline and ultimate death of retinal ganglion cells (RGCs). While multiple risk factors are associated with glaucoma, the mechanisms leading to onset and progression of the disease remain unknown. Molecular analysis in various...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3431380?pdf=render |
| _version_ | 1828529944193400832 |
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| author | Alejandra Bosco Samuel D Crish Michael R Steele Cesar O Romero Denise M Inman Philip J Horner David J Calkins Monica L Vetter |
| author_facet | Alejandra Bosco Samuel D Crish Michael R Steele Cesar O Romero Denise M Inman Philip J Horner David J Calkins Monica L Vetter |
| author_sort | Alejandra Bosco |
| collection | DOAJ |
| description | Glaucoma is a neurodegenerative disease that results in the progressive decline and ultimate death of retinal ganglion cells (RGCs). While multiple risk factors are associated with glaucoma, the mechanisms leading to onset and progression of the disease remain unknown. Molecular analysis in various glaucoma models has revealed involvement of non-neuronal cell populations, including astrocytes, Mueller glia and microglia, at early stages of glaucoma. High-dose irradiation was reported to have a significant long-term protective effect in the DBA/2J (D2) mouse model of glaucoma, although the cellular and molecular basis for this effect remains unclear. In particular, the acute effects of irradiation on specific cell populations, including non-neuronal cells, in the D2 retina and nerve have not been assessed. Here we report that irradiation induces transient reduction in proliferating microglia within the optic nerve head and glial lamina within the first week post-irradiation. This was accompanied by reduced microglial activation, with no effect on astrocyte gliosis in those regions. At later stages we confirm that early high-dose irradiation of the mouse head results in improvement of axonal structural integrity and anterograde transport function, without reduction of intraocular pressure. Thus reduced microglial activation induced by irradiation at early stages is associated with reduced optic nerve and retinal neurodegeneration in the D2 mouse model of glaucoma. |
| first_indexed | 2024-12-11T22:16:09Z |
| format | Article |
| id | doaj.art-3a10033f1acd459c89a9afb505aa51fa |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T22:16:09Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3a10033f1acd459c89a9afb505aa51fa2022-12-22T00:48:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4360210.1371/journal.pone.0043602Early reduction of microglia activation by irradiation in a model of chronic glaucoma.Alejandra BoscoSamuel D CrishMichael R SteeleCesar O RomeroDenise M InmanPhilip J HornerDavid J CalkinsMonica L VetterGlaucoma is a neurodegenerative disease that results in the progressive decline and ultimate death of retinal ganglion cells (RGCs). While multiple risk factors are associated with glaucoma, the mechanisms leading to onset and progression of the disease remain unknown. Molecular analysis in various glaucoma models has revealed involvement of non-neuronal cell populations, including astrocytes, Mueller glia and microglia, at early stages of glaucoma. High-dose irradiation was reported to have a significant long-term protective effect in the DBA/2J (D2) mouse model of glaucoma, although the cellular and molecular basis for this effect remains unclear. In particular, the acute effects of irradiation on specific cell populations, including non-neuronal cells, in the D2 retina and nerve have not been assessed. Here we report that irradiation induces transient reduction in proliferating microglia within the optic nerve head and glial lamina within the first week post-irradiation. This was accompanied by reduced microglial activation, with no effect on astrocyte gliosis in those regions. At later stages we confirm that early high-dose irradiation of the mouse head results in improvement of axonal structural integrity and anterograde transport function, without reduction of intraocular pressure. Thus reduced microglial activation induced by irradiation at early stages is associated with reduced optic nerve and retinal neurodegeneration in the D2 mouse model of glaucoma.http://europepmc.org/articles/PMC3431380?pdf=render |
| spellingShingle | Alejandra Bosco Samuel D Crish Michael R Steele Cesar O Romero Denise M Inman Philip J Horner David J Calkins Monica L Vetter Early reduction of microglia activation by irradiation in a model of chronic glaucoma. PLoS ONE |
| title | Early reduction of microglia activation by irradiation in a model of chronic glaucoma. |
| title_full | Early reduction of microglia activation by irradiation in a model of chronic glaucoma. |
| title_fullStr | Early reduction of microglia activation by irradiation in a model of chronic glaucoma. |
| title_full_unstemmed | Early reduction of microglia activation by irradiation in a model of chronic glaucoma. |
| title_short | Early reduction of microglia activation by irradiation in a model of chronic glaucoma. |
| title_sort | early reduction of microglia activation by irradiation in a model of chronic glaucoma |
| url | http://europepmc.org/articles/PMC3431380?pdf=render |
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