LncRNA Functional Screening in Organismal Development
Controversy continues over the functional prevalence of long non-coding RNAs (lncRNAs) despite their being widely investigated in all kinds of cells and organisms. In animals, lncRNAs have aroused general interest from exponentially increasing transcriptomic repertoires reporting their highly tissue...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Non-Coding RNA |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-553X/9/4/36 |
| _version_ | 1797583614047682560 |
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| author | Yang Li Huicong Zhai Lingxiu Tong Cuicui Wang Zhiming Xie Ke Zheng |
| author_facet | Yang Li Huicong Zhai Lingxiu Tong Cuicui Wang Zhiming Xie Ke Zheng |
| author_sort | Yang Li |
| collection | DOAJ |
| description | Controversy continues over the functional prevalence of long non-coding RNAs (lncRNAs) despite their being widely investigated in all kinds of cells and organisms. In animals, lncRNAs have aroused general interest from exponentially increasing transcriptomic repertoires reporting their highly tissue-specific and developmentally dynamic expression, and more importantly, from growing experimental evidence supporting their functionality in facilitating organogenesis and individual fitness. In mammalian testes, while a great multitude of lncRNA species are identified, only a minority of them have been shown to be useful, and even fewer have been demonstrated as true requirements for male fertility using knockout models to date. This noticeable gap is attributed to the virtual existence of a large number of junk lncRNAs, the lack of an ideal germline culture system, difficulty in loss-of-function interrogation, and limited screening strategies. Facing these challenges, in this review, we discuss lncRNA functionality in organismal development and especially in mouse testis, with a focus on lncRNAs with functional screening. |
| first_indexed | 2024-03-10T23:40:23Z |
| format | Article |
| id | doaj.art-3a1111378ad14d03ada024d869672b90 |
| institution | Directory Open Access Journal |
| issn | 2311-553X |
| language | English |
| last_indexed | 2024-03-10T23:40:23Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Non-Coding RNA |
| spelling | doaj.art-3a1111378ad14d03ada024d869672b902023-11-19T02:28:23ZengMDPI AGNon-Coding RNA2311-553X2023-06-01943610.3390/ncrna9040036LncRNA Functional Screening in Organismal DevelopmentYang Li0Huicong Zhai1Lingxiu Tong2Cuicui Wang3Zhiming Xie4Ke Zheng5State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, ChinaState Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, ChinaState Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, ChinaState Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, ChinaState Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, ChinaState Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, ChinaControversy continues over the functional prevalence of long non-coding RNAs (lncRNAs) despite their being widely investigated in all kinds of cells and organisms. In animals, lncRNAs have aroused general interest from exponentially increasing transcriptomic repertoires reporting their highly tissue-specific and developmentally dynamic expression, and more importantly, from growing experimental evidence supporting their functionality in facilitating organogenesis and individual fitness. In mammalian testes, while a great multitude of lncRNA species are identified, only a minority of them have been shown to be useful, and even fewer have been demonstrated as true requirements for male fertility using knockout models to date. This noticeable gap is attributed to the virtual existence of a large number of junk lncRNAs, the lack of an ideal germline culture system, difficulty in loss-of-function interrogation, and limited screening strategies. Facing these challenges, in this review, we discuss lncRNA functionality in organismal development and especially in mouse testis, with a focus on lncRNAs with functional screening.https://www.mdpi.com/2311-553X/9/4/36lncRNAfunctionscreeningdevelopmenttestis |
| spellingShingle | Yang Li Huicong Zhai Lingxiu Tong Cuicui Wang Zhiming Xie Ke Zheng LncRNA Functional Screening in Organismal Development Non-Coding RNA lncRNA function screening development testis |
| title | LncRNA Functional Screening in Organismal Development |
| title_full | LncRNA Functional Screening in Organismal Development |
| title_fullStr | LncRNA Functional Screening in Organismal Development |
| title_full_unstemmed | LncRNA Functional Screening in Organismal Development |
| title_short | LncRNA Functional Screening in Organismal Development |
| title_sort | lncrna functional screening in organismal development |
| topic | lncRNA function screening development testis |
| url | https://www.mdpi.com/2311-553X/9/4/36 |
| work_keys_str_mv | AT yangli lncrnafunctionalscreeninginorganismaldevelopment AT huicongzhai lncrnafunctionalscreeninginorganismaldevelopment AT lingxiutong lncrnafunctionalscreeninginorganismaldevelopment AT cuicuiwang lncrnafunctionalscreeninginorganismaldevelopment AT zhimingxie lncrnafunctionalscreeninginorganismaldevelopment AT kezheng lncrnafunctionalscreeninginorganismaldevelopment |