Antimicrobial Potential of Essential Oils from Aromatic Plant <i>Ocimum</i> sp.; A Comparative Biochemical Profiling and In-Silico Analysis
Medicinal and aromatic plants (MAPs) are a rich source of bioactive compounds that are immensely important due to their potential use in pharmacological and agricultural applications. Here, we have evaluated the antimicrobial activity of essential oils (EOs) from three different species of <i>...
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MDPI AG
2022-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/3/627 |
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| author | Prafull Salvi Gulshan Kumar Nishu Gandass Kajal Ashish Verma Sivasubramanian Rajarammohan Nilesh Rai Vibhav Gautam |
| author_facet | Prafull Salvi Gulshan Kumar Nishu Gandass Kajal Ashish Verma Sivasubramanian Rajarammohan Nilesh Rai Vibhav Gautam |
| author_sort | Prafull Salvi |
| collection | DOAJ |
| description | Medicinal and aromatic plants (MAPs) are a rich source of bioactive compounds that are immensely important due to their potential use in pharmacological and agricultural applications. Here, we have evaluated the antimicrobial activity of essential oils (EOs) from three different species of <i>Ocimum</i>: <i>O. gratissimum</i> (EO1), <i>O. tenuiflorum</i> (EO2), and <i>O. sanctum</i> (EO3). The EOs were screened for antibacterial activity against pathogenic strains of <i>Escherichia coli</i>, <i>Enterobacter cloacae</i> and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). The essential oils EO1 and EO3 showed significant growth inhibition of the tested bacteria. Likewise, all EOs exhibited antifungal potential against the broad-spectrum plant fungal pathogen <i>Sclerotinia sclerotiorum</i> that causes white-mould disease in plants. Moreover, the antimicrobial potential of the EOs correlates well with their antioxidant activity determined by DPPH free radical scavenging activity. The biochemical analysis of the EOs employing high-performance thin-layer chromatography, gas chromatography-mass spectrometry, and Fourier transform infrared spectroscopy, revealed the presence of distinct phytoconstituents that might be responsible for their differential bioactivity. Furthermore, an in-silico evaluation of the candidate phytoconstituents using molecular docking analysis suggests their potential for antimicrobial applications. Altogether, our results clearly show that EO1 and EO3 possess promising antimicrobial properties, and therefore could be utilized as a potential antimicrobial agent. |
| first_indexed | 2024-03-09T13:54:51Z |
| format | Article |
| id | doaj.art-3a1214eaca0a49399fda625504724966 |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T13:54:51Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-3a1214eaca0a49399fda6255047249662023-11-30T20:44:16ZengMDPI AGAgronomy2073-43952022-03-0112362710.3390/agronomy12030627Antimicrobial Potential of Essential Oils from Aromatic Plant <i>Ocimum</i> sp.; A Comparative Biochemical Profiling and In-Silico AnalysisPrafull Salvi0Gulshan Kumar1Nishu Gandass2Kajal3Ashish Verma4Sivasubramanian Rajarammohan5Nilesh Rai6Vibhav Gautam7Agri-Biotechnology, National Agri-Food Biotechnology Institute, Mohali 140306, IndiaAgri-Biotechnology, National Agri-Food Biotechnology Institute, Mohali 140306, IndiaAgri-Biotechnology, National Agri-Food Biotechnology Institute, Mohali 140306, IndiaAgri-Biotechnology, National Agri-Food Biotechnology Institute, Mohali 140306, IndiaCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, IndiaAgri-Biotechnology, National Agri-Food Biotechnology Institute, Mohali 140306, IndiaCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, IndiaCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, IndiaMedicinal and aromatic plants (MAPs) are a rich source of bioactive compounds that are immensely important due to their potential use in pharmacological and agricultural applications. Here, we have evaluated the antimicrobial activity of essential oils (EOs) from three different species of <i>Ocimum</i>: <i>O. gratissimum</i> (EO1), <i>O. tenuiflorum</i> (EO2), and <i>O. sanctum</i> (EO3). The EOs were screened for antibacterial activity against pathogenic strains of <i>Escherichia coli</i>, <i>Enterobacter cloacae</i> and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). The essential oils EO1 and EO3 showed significant growth inhibition of the tested bacteria. Likewise, all EOs exhibited antifungal potential against the broad-spectrum plant fungal pathogen <i>Sclerotinia sclerotiorum</i> that causes white-mould disease in plants. Moreover, the antimicrobial potential of the EOs correlates well with their antioxidant activity determined by DPPH free radical scavenging activity. The biochemical analysis of the EOs employing high-performance thin-layer chromatography, gas chromatography-mass spectrometry, and Fourier transform infrared spectroscopy, revealed the presence of distinct phytoconstituents that might be responsible for their differential bioactivity. Furthermore, an in-silico evaluation of the candidate phytoconstituents using molecular docking analysis suggests their potential for antimicrobial applications. Altogether, our results clearly show that EO1 and EO3 possess promising antimicrobial properties, and therefore could be utilized as a potential antimicrobial agent.https://www.mdpi.com/2073-4395/12/3/627<i>Ocimum</i> sp.essential oilantimicrobialHPTLCGC-MSFTIR |
| spellingShingle | Prafull Salvi Gulshan Kumar Nishu Gandass Kajal Ashish Verma Sivasubramanian Rajarammohan Nilesh Rai Vibhav Gautam Antimicrobial Potential of Essential Oils from Aromatic Plant <i>Ocimum</i> sp.; A Comparative Biochemical Profiling and In-Silico Analysis Agronomy <i>Ocimum</i> sp. essential oil antimicrobial HPTLC GC-MS FTIR |
| title | Antimicrobial Potential of Essential Oils from Aromatic Plant <i>Ocimum</i> sp.; A Comparative Biochemical Profiling and In-Silico Analysis |
| title_full | Antimicrobial Potential of Essential Oils from Aromatic Plant <i>Ocimum</i> sp.; A Comparative Biochemical Profiling and In-Silico Analysis |
| title_fullStr | Antimicrobial Potential of Essential Oils from Aromatic Plant <i>Ocimum</i> sp.; A Comparative Biochemical Profiling and In-Silico Analysis |
| title_full_unstemmed | Antimicrobial Potential of Essential Oils from Aromatic Plant <i>Ocimum</i> sp.; A Comparative Biochemical Profiling and In-Silico Analysis |
| title_short | Antimicrobial Potential of Essential Oils from Aromatic Plant <i>Ocimum</i> sp.; A Comparative Biochemical Profiling and In-Silico Analysis |
| title_sort | antimicrobial potential of essential oils from aromatic plant i ocimum i sp a comparative biochemical profiling and in silico analysis |
| topic | <i>Ocimum</i> sp. essential oil antimicrobial HPTLC GC-MS FTIR |
| url | https://www.mdpi.com/2073-4395/12/3/627 |
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